Rapid Fabrication of Protein Microarrays via Autogeneration and on-Chip Purification of Biotinylated Probes

2020 ◽  
Vol 9 (9) ◽  
pp. 2267-2273
Author(s):  
Bo-Wen Li ◽  
Yi Zhang ◽  
Yin-Chun Wang ◽  
Yang Xue ◽  
Xin-Yi Nie
Keyword(s):  
Protein Microarrays ◽  
Rapid Fabrication ◽  
On Chip ◽  
Biotinylated Probes

On-Chip Fabrication of None-Dead-Volume Microtips for ESI-MS Applications

2007 ◽  
Vol 121-123 ◽  
pp. 611-614
Author(s):  
Che Hsin Lin ◽  
Jen Taie Shiea ◽  
Yen Lieng Lin
Keyword(s):  
Surface Tension ◽  
Low Cost ◽  
Microfluidic Channel ◽  
Dead Volume ◽  
Esi Ms ◽  
Rapid Fabrication ◽  
Chip Fabrication ◽  
Novel Method ◽  
On Chip ◽  
Highly Uniform

This paper proposes a novel method to on-chip fabricate a none-dead-volume microtip for ESI-MS applications. The microfluidic chip and ESI tip are fabricated in low-cost plastic based materials using a simple and rapid fabrication process. A constant-speed-pulling method is developed to fabricate the ESI tip by pulling mixed PMMA glue using a 30-μm stainless wire through the pre-formed microfluidic channel. The equilibrium of surface tension of PMMA glue will result in a sharp tip after curing. A highly uniform micro-tip can be formed directly at the outlet of the microfluidic channel with minimum dead-volume zone. Detection of caffeine, myoglobin, lysozyme and cytochrome C biosamples confirms the microchip device can be used for high resolution ESI-MS applications.

Low cost rapid fabrication of vertical LVOF microspectrometer on-chip for MIR sensing

2018 ◽  
Author(s):  
Shurui Wang ◽  
Simon Chun Kiat Goh ◽  
Li Lynn Shiau ◽  
Nan Chen ◽  
Kailiang Chuan ◽  
...  
Keyword(s):  
Low Cost ◽  
Rapid Fabrication ◽  
On Chip

scholarly journals Microfluidic Rapid Fabrication of Tunable Polyvinyl Alcohol Microspheres for Adsorption Applications

Materials ◽  
2019 ◽  
Vol 12 (22) ◽  
pp. 3712 ◽  
Author(s):  
Jianmei Wang ◽  
Xueying Wang ◽  
Pingan Zhu ◽  
Chengmin Chen ◽  
Jianchun Wang ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Internal Structure ◽  
Ph Value ◽  
Channel Structure ◽  
Uniform Size ◽  
Rapid Fabrication ◽  
Pseudo Second Order ◽  
Facile Fabrication ◽  
Value Flow ◽  
On Chip

Monodisperse polyvinyl alcohol (PVA) microspheres have been widely used for targeted drug delivery, embolization, and templates. However, the fast and facile fabrication of PVA microspheres with uniform size and internal structure and good sphericity remains a challenge. In this study, the PVA microspheres with uniformity in the size, shape, and internal structure were rapidly fabricated, using single-emulsion droplet templates by an on-chip approach. First, we designed a polydimethylsiloxane (PDMS) microfluidic chip integrated with three functional units, used for the droplet generation, mixing of reagents, and pre-curing of PVA microspheres, respectively. Then, we precisely controlled the generation of PVA aqueous droplets, mixing of reagents, and the gelation rate for the production of high-quality microspheres by adjusting the pH value, flow rate, and the channel structure. The prepared PVA microspheres are characterized with good sphericity, uniform internal structure, and narrow size distribution. The microspheres have good adsorption capacity and recyclability for small-molecule drugs, as demonstrated by the adsorption and desorption of methylene blue (MB). The adsorption behavior is well described by the pseudo-second-order model, and intraparticle diffusion is as fast as the external film diffusion.

Gut microbiome a promising target for management of respiratory diseases

2020 ◽  
Vol 477 (14) ◽  
pp. 2679-2696
Author(s):  
Riddhi Trivedi ◽  
Kalyani Barve
Keyword(s):  
Respiratory Diseases ◽  
New Technology ◽  
Bacterial Flora ◽  
Systemic Circulation ◽  
The Body ◽  
Lung Pathology ◽  
Promising Target ◽  
Current Therapies ◽  
Intestinal Microbial Flora ◽  
On Chip

The intestinal microbial flora has risen to be one of the important etiological factors in the development of diseases like colorectal cancer, obesity, diabetes, inflammatory bowel disease, anxiety and Parkinson's. The emergence of the association between bacterial flora and lungs led to the discovery of the gut–lung axis. Dysbiosis of several species of colonic bacteria such as Firmicutes and Bacteroidetes and transfer of these bacteria from gut to lungs via lymphatic and systemic circulation are associated with several respiratory diseases such as lung cancer, asthma, tuberculosis, cystic fibrosis, etc. Current therapies for dysbiosis include use of probiotics, prebiotics and synbiotics to restore the balance between various species of beneficial bacteria. Various approaches like nanotechnology and microencapsulation have been explored to increase the permeability and viability of probiotics in the body. The need of the day is comprehensive study of mechanisms behind dysbiosis, translocation of microbiota from gut to lung through various channels and new technology for evaluating treatment to correct this dysbiosis which in turn can be used to manage various respiratory diseases. Microfluidics and organ on chip model are emerging technologies that can satisfy these needs. This review gives an overview of colonic commensals in lung pathology and novel systems that help in alleviating symptoms of lung diseases. We have also hypothesized new models to help in understanding bacterial pathways involved in the gut–lung axis as well as act as a futuristic approach in finding treatment of respiratory diseases caused by dysbiosis.

Proteomic profiling of the epithelial and stromal components of matched normal and colonic carcinomas using protein microarrays

Endoscopy ◽  
2006 ◽  
Vol 38 (11) ◽  
Author(s):  
KM Sheehan ◽  
C Gulmann ◽  
HL Barrett ◽  
EW Kay ◽  
LA Liotta ◽  
...  
Keyword(s):  
Protein Microarrays ◽  
Proteomic Profiling
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