scholarly journals Single-Step DNA Detection Assay Monitoring Dual-Color Light Scattering from Individual Metal Nanoparticle Aggregates

ACS Sensors ◽  
2017 ◽  
Vol 2 (2) ◽  
pp. 251-256 ◽  
Author(s):  
Stéphanie Vial ◽  
Youri Berrahal ◽  
Marta Prado ◽  
Jérôme Wenger
Keyword(s):  
Light Scattering ◽  
Dna Detection ◽  
Single Step ◽  
Metal Nanoparticle ◽  
Dual Color ◽  
Individual Metal ◽  
Detection Assay ◽  
Nanoparticle Aggregates

Single-step homogeneous immunoassay for detecting prostate-specific antigen using dual-color light scattering of metal nanoparticles

The Analyst ◽  
2017 ◽  
Vol 142 (18) ◽  
pp. 3484-3491 ◽  
Author(s):  
Stéphanie Vial ◽  
Jérôme Wenger
Keyword(s):  
Light Scattering ◽  
Metal Nanoparticles ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Single Step ◽  
Biomarker Detection ◽  
Protein Biomarker ◽  
Homogeneous Immunoassay ◽  
Dual Color ◽  
Sandwich Type

Conventional sandwich-type immunoassays are widely used for protein biomarker detection, yet their workflows are challenged by the need for multiple incubation steps separated by washing cycles.

scholarly journals Silver Nanoparticle-Based Assay for the Detection of Immunoglobulin Free Light Chains

Materials ◽  
2019 ◽  
Vol 12 (18) ◽  
pp. 2981 ◽  
Author(s):  
Anna Lizoń ◽  
Magdalena Wytrwal-Sarna ◽  
Marta Gajewska ◽  
Ryszard Drożdż
Keyword(s):  
Light Scattering ◽  
Silver Nanoparticle ◽  
Plasma Cells ◽  
Wide Spectrum ◽  
Metal Nanoparticle ◽  
Light Chains ◽  
Hydrodynamic Diameter ◽  
Al Amyloidosis ◽  
Free Light Chains ◽  
Optimal Test

There is a wide spectrum of malignant diseases that are connected with the clonal proliferation of plasma cells, which cause the production of complete immunoglobulins or their fragments (heavy or light immunoglobulin chains). These proteins may accumulate in tissues, leading to end organ damage. The quantitative determination of immunoglobulin free light chains (FLCs) is considered to be the gold standard in the detection and treatment of multiple myeloma (MM) and amyloid light-chain (AL) amyloidosis. In this study, a silver nanoparticle-based diagnostic tool for the quantitation of FLCs is presented. The optimal test conditions were achieved when a metal nanoparticle (MNP) was covered with 10 particles of an antibody and conjugated by 5–50 protein antigen particles (FLCs). The formation of the second antigen protein corona was accompanied by noticeable changes in the surface plasmon resonance spectra of the silver nanoparticles (AgNPs), which coincided with an increase of the hydrodynamic diameter and increase in the zeta potential, as demonstrated by dynamic light scattering (DLS). A decrease of repulsion forces and the formation of antigen–antibody bridges resulted in the agglutination of AgNPs, as demonstrated by transmission electron microscopy and the direct formation of AgNP aggregates. Antigen-conjugated AgNPs clusters were also found by direct observation using green laser light scattering. The parameters of the specific immunochemical aggregation process consistent with the sizes of AgNPs and the protein particles that coat them were confirmed by four physical methods, yielding complementary data concerning a clinically useful AgNPs aggregation test.

scholarly journals Cellular microRNA detection with miRacles: microRNA- activated conditional looping of engineered switches

2019 ◽  
Vol 5 (3) ◽  
pp. eaau9443 ◽  
Author(s):  
Arun Richard Chandrasekaran ◽  
Molly MacIsaac ◽  
Paromita Dey ◽  
Oksana Levchenko ◽  
Lifeng Zhou ◽  
...  
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Northern Blotting ◽  
Single Step ◽  
Single Nucleotide ◽  
Disease Biomarkers ◽  
Microrna Detection ◽  
Detection Assay ◽  
Polymerase Chain ◽  
Regulatory Rnas ◽  
Nucleotide Specificity

MicroRNAs are short noncoding regulatory RNAs that are increasingly used as disease biomarkers. Detection of microRNAs can be arduous and expensive and often requires amplification, labeling, or radioactive probes. Here, we report a single-step, nonenzymatic microRNA detection assay using conformationally responsive DNA nanoswitches. Termed miRacles (microRNA-activated conditional looping of engineered switches), our assay has subattomole sensitivity and single-nucleotide specificity using an agarose gel electrophoresis readout. We detect cellular microRNAs from nanogram-scale RNA extracts of differentiating muscle cells and multiplex our detection for several microRNAs from one biological sample. We demonstrate 1-hour detection without expensive equipment or reagents, making this assay a compelling alternative to quantitative polymerase chain reaction and Northern blotting.

Imaging Plasmon Hybridization in Metal Nanoparticle Aggregates with Electron Energy-Loss Spectroscopy

2016 ◽  
Vol 120 (37) ◽  
pp. 20852-20859 ◽  
Author(s):  
Steven C. Quillin ◽  
Charles Cherqui ◽  
Nicholas P. Montoni ◽  
Guoliang Li ◽  
Jon P. Camden ◽  
...  
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Electron Energy Loss Spectroscopy ◽  
Metal Nanoparticle ◽  
Plasmon Hybridization ◽  
Nanoparticle Aggregates ◽  
Electron Energy Loss

Simulation of ESR spectra of metal nanoparticle aggregates

2007 ◽  
Vol 69 (1) ◽  
pp. 9-12 ◽  
Author(s):  
S. V. Dolotov ◽  
V. I. Roldughin
Keyword(s):  
Esr Spectra ◽  
Metal Nanoparticle ◽  
Nanoparticle Aggregates

Plasmon Controlled Shaping of Metal Nanoparticle Aggregates by Femtosecond Laser-Induced Melting

2018 ◽  
Vol 9 (17) ◽  
pp. 5002-5008 ◽  
Author(s):  
D. Catone ◽  
A. Ciavardini ◽  
L. Di Mario ◽  
A. Paladini ◽  
F. Toschi ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Metal Nanoparticle ◽  
Nanoparticle Aggregates
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