scholarly journals Development of an Ultrasensitive HIV-1 DNA Detection Assay Based on an Automated πCode End-Point PCR System

2019 ◽  
Vol 1 (2) ◽  
Keyword(s):  
Dna Detection ◽  
End Point ◽  
Detection Assay ◽  
Hiv 1

Sub-attomolar HIV-1 DNA detection using surface-enhanced Raman spectroscopy

The Analyst ◽  
2010 ◽  
Vol 135 (5) ◽  
pp. 1084 ◽  
Author(s):  
Juan Hu ◽  
Peng-Cheng Zheng ◽  
Jian-Hui Jiang ◽  
Guo-Li Shen ◽  
Ru-Qin Yu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Dna Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Hiv 1

Incorporation of fetal DNA detection assay in a noninvasive RhD diagnostic test

2010 ◽  
Vol 30 (10) ◽  
pp. 1010-1012 ◽  
Author(s):  
Yuchi Li ◽  
Jeffrey A. Kazzaz ◽  
Leonard H. Kellner ◽  
Stephen A. Brown
Keyword(s):  
Diagnostic Test ◽  
Dna Detection ◽  
Fetal Dna ◽  
Detection Assay

Sensitive and selective viral DNA detection assay via microbead-based rolling circle amplification

2008 ◽  
Vol 18 (22) ◽  
pp. 5871-5874 ◽  
Author(s):  
Eric Schopf ◽  
Nicholas O. Fischer ◽  
Yong Chen ◽  
Jeffrey B.-H. Tok
Keyword(s):  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Viral Dna ◽  
Rolling Circle ◽  
Detection Assay

scholarly journals Magnesium Functions as Superior Co-factor for Measuring Reverse Transcriptase Activity of HIV-1, HIV-2, and SIV

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
Salequl Islam ◽  
Mohammad Ali Moni ◽  
Atsushi Tanaka ◽  
Hiroo Hoshino
Keyword(s):  
Reverse Transcriptase ◽  
Cell Line ◽  
Indirect Immunofluorescence ◽  
Dna Detection ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Detection Techniques ◽  
Proviral Dna ◽  
Infected Cells ◽  
Hiv 1

This study compared different detection methods of human/simian immunodeficiency virus (HIV/SIV) infections in the cell line systems; notably, i) Indirect immunofluorescence assay (IFA), ii) integrated proviral DNA detection, iii) detection of syncytia, iv) measurement of reverse transcriptase (RT) activity. RTs of various retroviruses require cations, including Mg2+, Mn2+, Ni2+, and Cu2+, for their enzyme-activities. The study further compared the roles of Mg2+ and Mn2+ as cofactors for RT activities of freshly harvested HIV-1, HIV-2, and SIV. The NP-2/CD4/coreceptor cells were seeded for overnight and infected with viral inoculums at a multiplicity of infection (MOI) 1.0. The cells were passaged regularly in a 2-3 days interval and maintained up to 2 weeks.  Infected cells were detected by indirect immunofluorescence assay (IFA). Multinucleated giant cells (MGC) in syncytia were quantified by Giemsa-staining. Proviral DNA was detected by PCR, and reverse transcriptase (RT) activity was measured.  Two different cations, Mg2+ and Mn2+ were used as cofactors for RT assay. We found all the strains of HIV-1, HV-2 and SIV to infection in the cell line conveniently. IFA had identified all the viral infections in the infected cells. Proviral DNA detection, syncytia formation was observed in the infected cells. We found a better performance of Mg2+ as cofactor over Mn2+ in RT assay for HIV-1, HIV-2, SIV. Different four detection techniques of HIV/SIV infections show high level of agreement in the NP-2-based cell line system. Mg2+ remains a better cofactor for RT.

Total HIV-1 DNA detection and quantification in peripheral blood by COBAS®AmpliPrep/COBAS® TaqMan® HIV-1 Test, v2.0

2016 ◽  
Vol 54 (2) ◽  
Author(s):  
Matteo Surdo ◽  
Ada Bertoli ◽  
Giuseppe Colucci ◽  
Loredana Sarmati ◽  
Massimo Andreoni ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Dna Detection ◽  
Hiv 1 ◽  
Detection And Quantification

Enhanced chemiluminescence-based hybridization analysis for PCR-mediated HIV-1 DNA detection offers an alternative to 32P-labelled probes

1991 ◽  
Vol 33 (3) ◽  
pp. 391-395 ◽  
Author(s):  
Vladimir Zachar ◽  
Vlastimil Mayer ◽  
George Aboagye-Mathiesen ◽  
Niels Nørskov-Lauritsen ◽  
Peter Ebbesen
Keyword(s):  
Dna Detection ◽  
Hybridization Analysis ◽  
Enhanced Chemiluminescence ◽  
Hiv 1

Analysis of the HIV-1 gp41 specific immune response using a multiplexed antibody detection assay

2004 ◽  
Vol 287 (1-2) ◽  
pp. 49-65 ◽  
Author(s):  
David Opalka ◽  
Antonello Pessi ◽  
Elisabetta Bianchi ◽  
Gennaro Ciliberto ◽  
William Schleif ◽  
...  
Keyword(s):  
Immune Response ◽  
Specific Immune Response ◽  
Antibody Detection ◽  
Detection Assay ◽  
Hiv 1
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