scholarly journals Highly Sensitive Fluorescence Assay for miRNA Detection: Investigation of the DNA Spacer Effect on the DSN Enzyme Activity toward Magnetic-Bead-Tethered Probes

ACS Omega ◽  
2022 ◽  
Author(s):  
Khouloud Djebbi ◽  
Biao Shi ◽  
Ting Weng ◽  
Mohamed Bahri ◽  
Mohamed Amin Elaguech ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Magnetic Bead ◽  
Fluorescence Assay ◽  
Highly Sensitive ◽  
Mirna Detection

A simple and highly sensitive fluorescence assay for microRNAs

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1932-1938 ◽  
Author(s):  
Wei Shen ◽  
Kiat Huei Yeo ◽  
Zhiqiang Gao
Keyword(s):  
Magnetic Bead ◽  
Dna Probes ◽  
Fluorescence Assay ◽  
Sensitive Assay ◽  
Highly Sensitive

A simple and highly sensitive assay for miRNAs using magnetic bead-carried DNA probes coupled with DSN-facilitated isothermal target cycling is reported.

Smartphone Based Highly Sensitive Visualized Detection of Acid Phosphatase Enzyme Activity

2021 ◽  
Author(s):  
Rui Li ◽  
Yanan Sun ◽  
Lihua Jin ◽  
Xiaohong Qiao ◽  
Cong Li ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Point Of Care ◽  
Rapid Development ◽  
Practical Application ◽  
Fast Analysis ◽  
Highly Sensitive ◽  
Visualized Detection ◽  
Sensitive Method ◽  
Phosphatase Enzyme

With the rapid development of point-of-care (POC) technologies, the improvement of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for the practical application. In...

Highly sensitive multiplex detection of microRNA by competitive DNA strand displacement fluorescence assay

Talanta ◽  
2019 ◽  
Vol 200 ◽  
pp. 487-493 ◽  
Author(s):  
Raja Chinnappan ◽  
Rawa Mohammed ◽  
Ahmed Yaqinuddin ◽  
Khalid Abu-Salah ◽  
Mohammed Zourob
Keyword(s):  
Fluorescence Assay ◽  
Multiplex Detection ◽  
Strand Displacement ◽  
Highly Sensitive ◽  
Dna Strand Displacement ◽  
Dna Strand

Highly sensitive antimonene based SPR biosensor for miRNA detection

2020 ◽  
Vol 28 ◽  
pp. 1776-1780
Author(s):  
Maneesh Kumar Singh ◽  
Sarika Pal ◽  
Y.K. Prajapati ◽  
J.P. Saini
Keyword(s):  
Spr Biosensor ◽  
Highly Sensitive ◽  
Mirna Detection

Development of a selective and highly sensitive fluorescence assay for nucleoside triphosphate diphosphohydrolase1 (NTPDase1, CD39)

The Analyst ◽  
2018 ◽  
Vol 143 (22) ◽  
pp. 5417-5430 ◽  
Author(s):  
Sang-Yong Lee ◽  
Xihuan Luo ◽  
Vigneshwaran Namasivayam ◽  
Jennifer Geiss ◽  
Salahuddin Mirza ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Nucleoside Triphosphate ◽  
Highly Sensitive

A new fluorescence-based assay is useful for monitoring CD39 reactions and enables low picomolar detection of nucleotides.

Development of a highly sensitive real-time immuno-PCR for the measurement of chloramphenicol in milk based on magnetic bead capturing

2014 ◽  
Vol 6 (23) ◽  
pp. 9340-9347 ◽  
Author(s):  
Xiaoqi Tao ◽  
Zhifei He ◽  
Xingyuan Cao ◽  
Jianzhong Shen ◽  
Hongjun Li
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Magnetic Bead ◽  
The Real ◽  
Highly Sensitive

The real-time immuno-quantitative PCR (RT-IPCR) schematic illustration of the determination of CAP in milk based on magnetic bead capturing.

scholarly journals Imaging multiple microRNAs in living cells using ATP self-powered strand-displacement cascade amplification

2018 ◽  
Vol 9 (5) ◽  
pp. 1184-1190 ◽  
Author(s):  
Xiangdan Meng ◽  
Wenhao Dai ◽  
Kai Zhang ◽  
Haifeng Dong ◽  
Xueji Zhang
Keyword(s):  
Living Cells ◽  
Strand Displacement ◽  
Displacement Cascade ◽  
Highly Sensitive ◽  
Mirna Detection ◽  
Self Powered ◽  
Cascade Amplification

Herein, we design a smart autonomous ATP self-powered strand displacement cascade amplification (SDCA) system for highly sensitive multiple intracellular miRNA detection.

Magnetic Spin Valve Sensors with Different Geometry to Magnetic Bead Detection for Biosensor Application

2007 ◽  
Vol 1032 ◽  
Author(s):  
Jeong Dae Suh ◽  
Myung Ae Chung
Keyword(s):  
Magnetic Beads ◽  
Spin Valve ◽  
Magnetic Bead ◽  
Highly Sensitive ◽  
Magnetic Spin ◽  
Biosensor Application ◽  
Detection Of Biomolecules ◽  
Meander Line ◽  
Cross Type ◽  
Line Type

AbstractWe have demonstrated the use of highly sensitive spin valve sensors for the detection of micron magnetic beads. By using a ring type, cross type, and meander line type sensors, we were able to detect the presence of 2.8 μm size magnetic beads in real time by direct measurement of magnetic dipole fields from magnetic beads. The sensitivity of the ring, cross and meander line sensors were obtained about 50 μV/Oe, 7 μV/Oe, 30 μV/Oe and sensor output signals of 50 μV , 30 μV, 90 μV were obtained in an external applied field of 10 Oe and 1 mA sense current. Our results shows that ring, cross, and meander line shape spin valve sensors are very promising candidates for the detection of biomolecules with magnetic labels.

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