A simple and highly sensitive fluorescence assay for microRNAs

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1932-1938 ◽  
Author(s):  
Wei Shen ◽  
Kiat Huei Yeo ◽  
Zhiqiang Gao
Keyword(s):  
Magnetic Bead ◽  
Dna Probes ◽  
Fluorescence Assay ◽  
Sensitive Assay ◽  
Highly Sensitive

A simple and highly sensitive assay for miRNAs using magnetic bead-carried DNA probes coupled with DSN-facilitated isothermal target cycling is reported.

Magnetic bead extraction with acid dissociation immunoassay for the determination of serum CD80-Fc fusion protein

Bioanalysis ◽  
2020 ◽  
Author(s):  
Rosanna Kwok ◽  
Ago B Ahene ◽  
Mason Brown ◽  
Christopher Hunter
Keyword(s):  
Fusion Protein ◽  
T Lymphocyte ◽  
Magnetic Beads ◽  
Cytotoxic T Lymphocyte ◽  
Theoretical Calculations ◽  
Magnetic Bead ◽  
Acid Dissociation ◽  
Sensitive Assay ◽  
Highly Sensitive ◽  
Fc Fusion Protein

Background: To detect concentrations of subtherapeutic doses of the CD80-Fc fusion protein FPT155 in serum in Phase I studies, a highly sensitive assay was developed. Materials & methods: FPT155 was purified from human serum using magnetic beads coupled to cytotoxic T-lymphocyte-associated antigen-4. After washing away the serum components, FTP155 was released by acid dissociation and neutralization. The eluted drug was quantified in an ELISA using cytotoxic T-lymphocyte-associated antigen-4 as a capture reagent and biotinylated anti-human Fc for detection. The assay was validated with a calibration range of 5–40 ng/ml and a dilutional integrity of up to 100,000 ng/ml. Conclusion: A highly sensitive assay to determine serum concentrations of FPT155 using readily available reagents was developed. The results were in conformity with theoretical calculations.

Application strategies of peptide nucleic acids toward electrochemical nucleic acid sensors

The Analyst ◽  
2021 ◽  
Author(s):  
Qingteng Lai ◽  
Wei Chen ◽  
Yanke Zhang ◽  
Zheng-Chun Liu
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Dna Probes ◽  
Highly Sensitive ◽  
Increased Sensitivity ◽  
Acid Sensors

Peptide nucleic acids (PNAs) have attracted tremendous interest in the fabrication of highly sensitive electrochemical nucleic acid biosensor due to their higher stability and increased sensitivity than common DNA probes....

Highly sensitive multiplex detection of microRNA by competitive DNA strand displacement fluorescence assay

Talanta ◽  
2019 ◽  
Vol 200 ◽  
pp. 487-493 ◽  
Author(s):  
Raja Chinnappan ◽  
Rawa Mohammed ◽  
Ahmed Yaqinuddin ◽  
Khalid Abu-Salah ◽  
Mohammed Zourob
Keyword(s):  
Fluorescence Assay ◽  
Multiplex Detection ◽  
Strand Displacement ◽  
Highly Sensitive ◽  
Dna Strand Displacement ◽  
Dna Strand

A highly sensitive assay for adenosine triphosphate employing an improved firefly luciferase reagent

1990 ◽  
Vol 11 (4) ◽  
pp. 208-210 ◽  
Author(s):  
W. J. Simpson ◽  
J. L. Fernandez ◽  
J. R. M. Hammond ◽  
P. S. Senior ◽  
B. J. McCarthy ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Firefly Luciferase ◽  
Sensitive Assay ◽  
Highly Sensitive

Use of salt-stored zonae pellucidae as a highly sensitive assay of immunological infertility

1988 ◽  
Vol 29 (1) ◽  
pp. 246 ◽  
Author(s):  
R.A. Fayrer-Hosken ◽  
C.E. McBride ◽  
B.G. Brackett
Keyword(s):  
Sensitive Assay ◽  
Immunological Infertility ◽  
Highly Sensitive
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