scholarly journals Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

ACS Omega ◽  
2021 ◽  
Author(s):  
Pedro A. Duarte ◽  
Lukas Menze ◽  
Lian Shoute ◽  
Jie Zeng ◽  
Oleksandra Savchenko ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogen ◽  
Highly Efficient ◽  
Microwell Array

scholarly journals An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans

mSphere ◽  
2017 ◽  
Vol 2 (2) ◽  
Author(s):  
Namkha Nguyen ◽  
Morgan M. F. Quail ◽  
Aaron D. Hernday
Keyword(s):  
Candida Albicans ◽  
Genome Editing ◽  
Fungal Pathogen ◽  
Gene Knockout ◽  
Strain Engineering ◽  
Molecular Genetic Analysis ◽  
Content Type ◽  
Highly Efficient ◽  
Discovery Research ◽  
Gene Knockouts

ABSTRACT Candida albicans is the most common fungal pathogen of humans. Historically, molecular genetic analysis of this important pathogen has been hampered by the lack of stable plasmids or meiotic cell division, limited selectable markers, and inefficient methods for generating gene knockouts. The recent development of clustered regularly interspaced short palindromic repeat(s) (CRISPR)-based tools for use with C. albicans has opened the door to more efficient genome editing; however, previously reported systems have specific limitations. We report the development of an optimized CRISPR-based genome editing system for use with C. albicans. Our system is highly efficient, does not require molecular cloning, does not leave permanent markers in the genome, and supports rapid, precise genome editing in C. albicans. We also demonstrate the utility of our system for generating two independent homozygous gene knockouts in a single transformation and present a method for generating homozygous wild-type gene addbacks at the native locus. Furthermore, each step of our protocol is compatible with high-throughput strain engineering approaches, thus opening the door to the generation of a complete C. albicans gene knockout library. IMPORTANCE Candida albicans is the major fungal pathogen of humans and is the subject of intense biomedical and discovery research. Until recently, the pace of research in this field has been hampered by the lack of efficient methods for genome editing. We report the development of a highly efficient and flexible genome editing system for use with C. albicans. This system improves upon previously published C. albicans CRISPR systems and enables rapid, precise genome editing without the use of permanent markers. This new tool kit promises to expedite the pace of research on this important fungal pathogen.

scholarly journals Host-Induced Gene Silencing of a Multifunction Gene Sscnd1 Enhances Plant Resistance Against Sclerotinia sclerotiorum

2021 ◽  
Vol 12 ◽  
Author(s):  
Yijuan Ding ◽  
Yangui Chen ◽  
Baoqin Yan ◽  
Hongmei Liao ◽  
Mengquan Dong ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogen ◽  
Crop Production ◽  
Tobacco Rattle Virus ◽  
Cell Integrity ◽  
Sclerotinia Stem Rot ◽  
Appressorium Formation ◽  
Stem Loop ◽  
Infection Stage

Sclerotinia sclerotiorum is a devastating necrotrophic fungal pathogen and has a substantial economic impact on crop production worldwide. Magnaporthe appressoria-specific (MAS) proteins have been suggested to be involved in the appressorium formation in Magnaporthe oryzae. Sscnd1, an MAS homolog gene, is highly induced at the early infection stage of S. sclerotiorum. Knock-down the expression of Sscnd1 gene severely reduced the virulence of S. sclerotiorum on intact rapeseed leaves, and their virulence was partially restored on wounded leaves. The Sscnd1 gene-silenced strains exhibited a defect in compound appressorium formation and cell integrity. The instantaneous silencing of Sscnd1 by tobacco rattle virus (TRV)-mediated host-induced gene silencing (HIGS) resulted in a significant reduction in disease development in tobacco. Three transgenic HIGS Arabidopsis lines displayed high levels of resistance to S. sclerotiorum and decreased Sscnd1 expression. Production of specific Sscnd1 siRNA in transgenic HIGS Arabidopsis lines was confirmed by stem-loop qRT-PCR. This study revealed that the compound appressorium-related gene Sscnd1 is required for cell integrity and full virulence in S. sclerotiorum and that Sclerotinia stem rot can be controlled by expressing the silencing constructs of Sscnd1 in host plants.

scholarly journals Genome sequence resource for the plant pathogen Sclerotinia sclerotiorum WH6 isolated in China

Plant Disease ◽  
2021 ◽  
Author(s):  
Xiong Zhang ◽  
Xiaohui Cheng ◽  
Lijiang Liu ◽  
Shengyi Liu
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Genome Sequence ◽  
Fungal Pathogen ◽  
Sclerotinia Stem Rot ◽  
High Quality ◽  
Total Size ◽  
Host Interactions ◽  
A Genome ◽  
The World ◽  
High Quality Genome

Sclerotinia sclerotiorum is a notorious fungal pathogen that causes sclerotinia stem rot (SSR) on many important crops in China and worldwide. Here, we present a high- quality genome assembly of S. sclerotiorum strain WH6 using the PacBio SMRT cell platform. The assembled genome has a total size of 38.96 Mbp, with a contig N50 length of 1.90 Mbp, and encodes 10,512 predicted coding genes, including 685 secreted proteins and 65 effector candidates. This is the the first report of a genome sequence from China. The WH6 genome sequence provides a valuable resource for facilitating our understanding of S. sclerotiorum-host interactions and SSR control in China and the world.

scholarly journals Identification of sources of Sclerotinia sclerotiorum resistance in a collection of wild Cicer germplasm

Plant Disease ◽  
2021 ◽  
Author(s):  
Virginia Wainaina Mwape ◽  
Yuphin Khentry ◽  
Toby E. Newman ◽  
Matthew Denton-Giles ◽  
Mark Derbyshire ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogen ◽  
Yield Losses ◽  
Sclerotinia Stem Rot ◽  
Collection Site ◽  
Breeding Programs ◽  
Cicer Arietinum L ◽  
Cicer Echinospermum ◽  
Stem Resistance ◽  
Wild Progenitors

Sclerotinia sclerotiorum is an important fungal pathogen of chickpea (Cicer arietinum L.) and it can cause yield losses up to 100%. The wild progenitors are much more diverse than domesticated chickpea and this study describes how this relates to S. sclerotiorum resistance. Initially, the pathogenicity of nine Australian S. sclerotiorum isolates was examined on three Cicer lines to develop a robust phenotyping assay and significant differences in isolate aggressiveness were identified with 6 isolates being classed as highly aggressive and 3 as moderately aggressive. We identified two S. sclerotiorum isolates, CU8.20 and CU10.12, to be highly aggressive and moderately aggressive, respectively. A subsequent phenotyping assay was conducted using the two isolates to evaluate 86 wild Cicer accessions (Cicer reticulatum and Cicer echinospermum) and two C. arietinum varieties for resistance to S. sclerotiorum. A subset of 12 genotypes was further evaluated, and subsequently, two wild Cicer accessions with consistently high levels of resistance to S. sclerotiorum were examined using the initially characterised nine isolates. Wild Cicer accessions Karab_084 and Deste_063 demonstrated consistent partial resistance to S. sclerotiorum. There were significant differences in responses to S. sclerotiorum across wild Cicer collection sites. The Cermik, Karabahce and Destek sites’ responses to the aggressive isolate CU8.20 ranged from resistant to susceptible, highlighting an interaction between isolate genotype and chickpea collection site for sclerotinia stem rot resistance. This is the first evidence of partial stem resistance identified in wild Cicer germplasm, which can be adopted in chickpea breeding programs to enhance S. sclerotiorum resistance in future chickpea varieties.

scholarly journals The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum

2015 ◽  
Vol 169 (1) ◽  
pp. 856-872 ◽  
Author(s):  
Chenggang Wang ◽  
Jin Yao ◽  
Xuezhu Du ◽  
Yanping Zhang ◽  
Yijun Sun ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogen ◽  
Mediator Complex ◽  
Basal Resistance

scholarly journals A novel virus that infecting hypovirulent strain XG36-1 of plant fungal pathogen Sclerotinia sclerotiorum

2009 ◽  
Vol 6 (1) ◽  
pp. 96 ◽  
Author(s):  
Liyan Zhang ◽  
Yanping Fu ◽  
Jiatao Xie ◽  
Daohong Jiang ◽  
Guoqing Li ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogen ◽  
Hypovirulent Strain ◽  
Novel Virus ◽  
Plant Fungal Pathogen

scholarly journals Characterization of Sclerotinia sclerotiorum, an Emerging Fungal Pathogen Causing Blight in Hyacinth Bean (Lablab purpureus)

2018 ◽  
Vol 34 (5) ◽  
pp. 367-380 ◽  
Author(s):  
Ananya Prova ◽  
Abdul Mannan Akanda ◽  
Shaikhul Islam ◽  
Md. Motaher Hossain
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogen ◽  
Lablab Purpureus ◽  
Hyacinth Bean

scholarly journals Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection

2012 ◽  
Vol 11 (3) ◽  
pp. 2171-2181 ◽  
Author(s):  
E.A. Bueno ◽  
M.B. Oliveira ◽  
R.V. Andrade ◽  
M. Lobo Júnior ◽  
S. Petrofeza
Keyword(s):  
Phaseolus Vulgaris ◽  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogen ◽  
Carbon Sources

scholarly journals A Novel Deltaflexivirus that Infects the Plant Fungal Pathogen, Sclerotinia sclerotiorum, Can Be Transmitted Among Host Vegetative Incompatible Strains

Viruses ◽  
2018 ◽  
Vol 10 (6) ◽  
pp. 295 ◽  
Author(s):  
Muhammad Hamid ◽  
Jiatao Xie ◽  
Songsong Wu ◽  
Shahzeen Maria ◽  
Dan Zheng ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogen ◽  
Plant Fungal Pathogen
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