scholarly journals Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection

2012 ◽  
Vol 11 (3) ◽  
pp. 2171-2181 ◽  
Author(s):  
E.A. Bueno ◽  
M.B. Oliveira ◽  
R.V. Andrade ◽  
M. Lobo Júnior ◽  
S. Petrofeza
Keyword(s):  
Phaseolus Vulgaris ◽  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogen ◽  
Carbon Sources

Efficacy of three greenhouse screening methods for the identification of physiological resistance to white mold in dry bean

2009 ◽  
Vol 89 (4) ◽  
pp. 755-762 ◽  
Author(s):  
H Terán ◽  
S P Singh
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Sclerotinia Sclerotiorum ◽  
Screening Method ◽  
White Mold ◽  
Screening Methods ◽  
Post Inoculation ◽  
Dry Bean ◽  
Physiological Resistance ◽  
Nueva Granada

White mold (WM) caused by Sclerotinia sclerotiorum (Lib.) de Bary is the most devastating disease of common bean (dry and snap or garden bean) (Phaseolus vulgaris L.) in North America. The use of a reliable screening method (SM) in common bean is crucial to improve physiological resistance to WM. The objective of this study was to compare the efficacy of three SM to identify physiological resistance in dry bean genotypes with different evolutionary origins and levels of resistance. Screening methods tested were: (i) the modified straw test or cut–stem (CSM); (ii) infected bean flower (IFL); and (iii) infected oat seed (IOS). A 195, ICA Bunsi, Othello, and VCW 54 dry bean were tested with the three SM. The experimental design was a split plot in randomized complete blocks with three replications in 2007 and 2008. Two independent inoculations 1 wk apart for each SM were made. The WM reaction was scored at 16, 23, and 33 d post-inoculation (DPI) using a 1 to 9 scale. There were highly significant differences between SM and its interaction with years. The CSM and IFL were the most consistent and highly correlated (r > 0.70, P < 0.01). Interspecific breeding line VCW 54 consistently had the highest WM resistance across years, SM, and evaluation dates, followed by A 195. White mold scores increased with delayed evaluations. Thus, CSM or IFL with disease assessed 33 DPI should be used for identifying common bean genotypes with high levels of physiological resistance to WM.Key words: Common bean, growth habit, race Mesoamerica, race Nueva Granada, Phaseolus vulgaris, Sclerotinia sclerotiorum

scholarly journals Aspergillus fumigatus Acetate Utilization Impacts Virulence Traits and Pathogenicity

mBio ◽  
2021 ◽  
Author(s):  
Laure Nicolas Annick Ries ◽  
Patricia Alves de Castro ◽  
Lilian Pereira Silva ◽  
Clara Valero ◽  
Thaila Fernanda dos Reis ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Fungal Pathogen ◽  
Carbon Sources ◽  
Body Fluids ◽  
Peripheral Tissues ◽  
Content Type ◽  
Virulence Traits ◽  
Acetate Utilization ◽  
Opportunistic Fungal Pathogen

Aspergillus fumigatus is an opportunistic fungal pathogen in humans. During infection, A. fumigatus is predicted to use host carbon sources, such as acetate, present in body fluids and peripheral tissues, to sustain growth and promote colonization and invasion.

Studies on the possible relationships of microbodies and multivesicular bodies to oxalate, endopolygalacturonase, and cellulase (Cx) production by Sclerotinia sclerotiorum

1972 ◽  
Vol 50 (8) ◽  
pp. 1743-1748 ◽  
Author(s):  
Douglas P. Maxwell ◽  
Paul H. Williams ◽  
Martha D. Maxwell
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Enzyme Production ◽  
Functional Role ◽  
Carbon Sources ◽  
Extracellular Enzyme ◽  
Ultrastructural Organization ◽  
Multivesicular Bodies ◽  
Membrane Bound ◽  
High Production

The possible functional role of vesicles and crystal-containing microbodies in the production of oxalate, endopolygalacturonase, or cellulase by Sclerotinia sclerotiorum was investigated. The presence of multivesicular bodies in hyphal tips was not correlated with secretion or production of oxalate or these extracellular hydrolases. More crystal-containing microbodies were present in hyphal tips grown on media which supported greater extracellular enzyme production. No correlation existed between numbers of crystal-containing microbodies in hyphal tips and production of oxalate. Numerous membrane-bound vesicles (0.09–0.18 µm diam) were associated with tips grown on a D-glucose–Na succinate medium which supported high production of oxalate. The general ultrastructural organization of these hyphal tips was similar to that reported for other ascomycetes. Differences in numbers and distributions of organelles were observed between hyphal tips and older hyphae as well as between hyphal tips grown on the different carbon sources.

scholarly journals Anaerobic Soil Disinfestation Reduces Viability of Sclerotinia sclerotiorum and S. minor Sclerotia and Root-Knot Nematodes in Muck Soils

2020 ◽  
Vol 110 (4) ◽  
pp. 795-804
Author(s):  
Andres D. Sanabria-Velazquez ◽  
Anna L. Testen ◽  
Ram B. Khadka ◽  
Zhe Liu ◽  
Fuqing Xu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sclerotinia Sclerotiorum ◽  
Wheat Bran ◽  
Carbon Sources ◽  
Ethanol Solution ◽  
Meloidogyne Hapla ◽  
Root Knot Nematodes ◽  
Soil Disinfestation ◽  
Anaerobic Soil Disinfestation ◽  
Muck Soil

Experiments were conducted to evaluate potential functional and mechanistic differences in the suppression of Sclerotinia sclerotiorum and S. minor and root-knot nematodes in muck soils by anaerobic soil disinfestation (ASD) using different carbon source amendments. Volatile compounds produced during ASD in muck soil amended with molasses, wheat bran, or mustard greens at 20.2 Mg/ha or a 2% ethanol solution significantly reduced the mycelial growth and number of sclerotia produced by both Sclerotinia spp. compared with the anaerobic control. In amended soils, acetic and butyric acids were detected in concentrations that reduced the viability of sclerotia of both pathogens. Higher concentrations of carbon dioxide were observed in ASD-treated soils, regardless of the amendment, than in the nonamended anaerobic control. Only amendment with wheat bran did not increase the production of methane gas during ASD compared with the controls. Meloidogyne hapla survival was completely suppressed in soils treated with ASD regardless of carbon source. Field trials were conducted in Ohio muck soil to assess survival of sclerotia of both Sclerotinia spp. The viability of sclerotia of both Sclerotinia spp. was significantly reduced in soil subjected to ASD amended with wheat bran (20.2 Mg/ha), molasses (10.1 Mg/ha), or wheat bran (20.2 Mg/ha) plus molasses (10.1 Mg/ha) compared with the controls. A consistent negative correlation between soil reduction and viability of sclerotia of both pathogens was observed. Wheat bran and molasses are both widely available amendments that can be used as ASD carbon sources for the management of soilborne pathogens in muck soils.

Numbers and distribution of apothecia of Sclerotinia sclerotiorum in relation to white mold of white bean (Phaseolus vulgaris)

1988 ◽  
Vol 66 (2) ◽  
pp. 247-252 ◽  
Author(s):  
G. J. Boland ◽  
R. Hall
Keyword(s):  
Phaseolus Vulgaris ◽  
Sclerotinia Sclerotiorum ◽  
Negative Binomial Distribution ◽  
Negative Binomial ◽  
Disease Incidence ◽  
White Mold ◽  
Spatial Distributions ◽  
Phaseolus Vulgaris L ◽  
Field Plot ◽  
White Bean

Relationships between the distribution and numbers of apothecia of Sclerotinia sclerotiorum (Lib.) de Bary and the distribution and incidence of white mold of white bean (Phaseolus vulgaris L.) were investigated in a field plot at Arkell, Ont., in 1981 and 1982. The spatial distributions of both apothecia and disease were aggregated and were most consistently described by the negative binomial distribution, although the Poisson, Poisson binomial and logarithmic with zeros distributions were also significant for some sampling dates. Numbers of apothecia were correlated with disease incidence within areas 1.4 (r = 0.07–0.67), 36 (r = 0.11–0.72), and 108 m2 (r = 0.21–0.95). The quantitative relationships between apothecia within a field of beans and the incidence of white mold may be useful in predicting the disease.

Response of bush and upright plant type selections to white mold and seed yield of common beans grown in various row widths in southern Ontario

1993 ◽  
Vol 73 (1) ◽  
pp. 265-272 ◽  
Author(s):  
S. J. Park
Keyword(s):  
Phaseolus Vulgaris ◽  
Sclerotinia Sclerotiorum ◽  
Seed Yield ◽  
Genetic Resistance ◽  
White Mold ◽  
Plant Type ◽  
Lodging Resistance ◽  
Row Width ◽  
Dry Edible Bean ◽  
Canopy Type

White mold, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is an important disease in common bean (Phaseolus vulgaris L.) production areas. It reduces seed yield and lowers the quality of beans. Development of the disease is thought to be affected by plant canopy type (architecture), and by genetic resistance. This study determined the effect of plant type and row width on white mold and seed yield. Three short bush/vine and four semi-determinate upright genotypes were tested in narrow (30 cm) and wide (60 and 80 cm) rows for 2 yr in a field prone to white mold development. Incidence and disease index increased as row width became narrower. The upright type was infected less than the short bush type. Ex Rico 23, which is a short vine, open canopy type, was moderately resistant to the mold. A tall upright selection, A55, showed the lowest mold infection; it was significantly less than that of Ex Rico 23. Seed yield of all genotypes increased as row width decreased even though there was an increase in white mold incidence. Stiff-strawed upright types had less lodging. To reduce white mold, cultivars should have lodging resistance, narrow canopied upright plant type and genetic resistance. Development of the upright plant types allows for narrow-row production and direct combining. Key words: Dry edible bean, Phaseolus vulgaris, white mold, Sclerotinia sclerotiorum, plant type, row width, seed yield

Sign in / Sign up

Export Citation Format

Share Document