Open Gate of Corynebacterium glutamicum Threonine Deaminase for Efficient Synthesis of Bulky α-Keto Acids

ACS Catalysis ◽  
2020 ◽  
Vol 10 (17) ◽  
pp. 9994-10004
Author(s):  
Wei Song ◽  
Xin Xu ◽  
Cong Gao ◽  
Yuxuan Zhang ◽  
Jing Wu ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Efficient Synthesis ◽  
Threonine Deaminase ◽  
Keto Acids

scholarly journals Sustainable synthesis of 3-substituted phthalides via a catalytic one-pot cascade strategy from 2-formylbenzoic acid with β-keto acids in glycerol

2017 ◽  
Vol 13 ◽  
pp. 1425-1429 ◽  
Author(s):  
Lina Jia ◽  
Fuzhong Han
Keyword(s):  
Natural Products ◽  
Cascade Reaction ◽  
Catalytic Process ◽  
Efficient Synthesis ◽  
One Pot ◽  
Keto Acids ◽  
Synthesis Strategy ◽  
Therapeutic Activities

Background: Phthalides are privileged constituents of numerous pharmaceuticals, natural products and agrochemicals and exhibit several biological and therapeutic activities. Therefore, the development of new, facile, and sustainable strategies for the construction of these moieties is highly desired. Results: A broad substrate scope for β-keto acids was found to be strongly compatible with this catalytic process, affording a wide variety of 3-substituted phthalides in good to excellent yields. Conclusion: A concise and efficient synthesis strategy of 3-substituted phthalides from 2-formylbenzoic acid and β-keto acids via a catalytic one-pot cascade reaction in glycerol has been accomplished.

scholarly journals Radical Carbonylative Synthesis of Heterocycles by Visible Light Photoredox Catalysis

Catalysts ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1054
Author(s):  
Xiao-Qiang Hu ◽  
Zi-Kui Liu ◽  
Wen-Jing Xiao
Keyword(s):  
Visible Light ◽  
Efficient Synthesis ◽  
Acyl Radical ◽  
Acyl Chlorides ◽  
Aryl Radicals ◽  
Keto Acids ◽  
Acyl Radicals ◽  
Different Types ◽  
The Impact ◽  
Visible Light Photocatalytic

Visible light photocatalytic radical carbonylation has been established as a robust tool for the efficient synthesis of carbonyl-containing compounds. Acyl radicals serve as the key intermediates in these useful transformations and can be generated from the addition of alkyl or aryl radicals to carbon monoxide (CO) or various acyl radical precursors such as aldehydes, carboxylic acids, anhydrides, acyl chlorides or α-keto acids. In this review, we aim to summarize the impact of visible light-induced acyl radical carbonylation reactions on the synthesis of oxygen and nitrogen heterocycles. The discussion is mainly categorized based on different types of acyl radical precursors.

Efficient Synthesis of Enantiopure β-Amino-γ-Keto Acids froml-Homoserine

2003 ◽  
Vol 5 (12) ◽  
pp. 2107-2109 ◽  
Author(s):  
Anil K. Sharma ◽  
Paul J. Hergenrother
Keyword(s):  
Efficient Synthesis ◽  
Keto Acids

scholarly journals Metabolic Engineering of Corynebacterium glutamicum for 2-Ketoisovalerate Production

2010 ◽  
Vol 76 (24) ◽  
pp. 8053-8061 ◽  
Author(s):  
Felix S. Krause ◽  
Bastian Blombach ◽  
Bernhard J. Eikmanns
Keyword(s):  
Corynebacterium Glutamicum ◽  
Competitive Inhibition ◽  
Pyruvate Dehydrogenase Complex ◽  
Product Yield ◽  
Acetohydroxyacid Synthase ◽  
Gene Encoding ◽  
Keto Acids ◽  
Genes Encoding ◽  
Cell Densities ◽  
Branched Chain

ABSTRACT 2-Ketoisovalerate is used as a therapeutic agent, and a 2-ketoisovalerate-producing organism may serve as a platform for products deriving from this 2-keto acid. We engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the l-valine biosynthetic enzymes acetohydroxyacid synthase (AHAS), acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production was further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 188 ± 28 mM (21.8 ± 3.2 g liter−1) 2-ketoisovalerate and showed a product yield of about 0.47 ± 0.05 mol per mol (0.3 ± 0.03 g per g) of glucose and a volumetric productivity of about 4.6 ± 0.6 mM (0.53 ± 0.07 g liter−1) 2-ketoisovalerate per h in the overall production phase. In studying the influence of the three branched-chain 2-keto acids 2-ketoisovalerate, 2-ketoisocaproate, and 2-keto-3-methylvalerate on the AHAS activity, we observed a competitive inhibition of the AHAS enzyme by 2-ketoisovalerate.

scholarly journals Efficient Synthesis of Enantiopure β-Amino-γ-Keto Acids from L-Homoserine.

ChemInform ◽  
2003 ◽  
Vol 34 (40) ◽  
Author(s):  
Anil K. Sharma ◽  
Paul J. Hergenrother
Keyword(s):  
Efficient Synthesis ◽  
Keto Acids
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