scholarly journals Calcium Carbonate/Gelatin Methacrylate Microspheres for 3D Cell Culture in Bone Tissue Engineering

2020 ◽  
Vol 26 (8) ◽  
pp. 418-432
Author(s):  
Pengwei Xu ◽  
Fuliang Jiang ◽  
Hongbo Zhang ◽  
Ruixue Yin ◽  
Lian Cen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Calcium Carbonate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
3D Cell Culture

Hierarchically Porous Calcium Carbonate Scaffolds for Bone Tissue Engineering

2017 ◽  
Vol 3 (10) ◽  
pp. 2457-2469 ◽  
Author(s):  
Abiy D. Woldetsadik ◽  
Sudhir K. Sharma ◽  
Sachin Khapli ◽  
Ramesh Jagannathan ◽  
Mazin Magzoub
Keyword(s):  
Tissue Engineering ◽  
Calcium Carbonate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Hierarchically Porous ◽  
Porous Calcium Carbonate

Preparation and Properties on Silk Fibers Reinforced Hydroxyapatite/Chitosan Composites

2010 ◽  
Vol 105-106 ◽  
pp. 557-560 ◽  
Author(s):  
Jing Wang ◽  
Q.Z. Sun ◽  
Jing Gao ◽  
D.M. Liu ◽  
Xiang Cai Meng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Compressive Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biological Properties ◽  
X Rays ◽  
Silk Fibers ◽  
Initial Strength ◽  
Scaffold Materials

Silk fibers were introduced into hydroxyapatite(HA)/chitosan(CS) matrix to prepare scaffold materials of bone tissue engineering with the adequate initial strength and improved cellular affinity using combination of in situ synthesis and freeze-drying technique. Chemical component was investigated using X rays diffraction (XRD) and Fourier transform infrared spectrum (FTIR). Structure and morphology of the composites were observed by scanning electron microscope (SEM). Porosity was tested by liquid substitution method. The mechanical properties of the composites were also measured. The simulated body fluid (SBF) and the cell culture experiments were conducted to assess biological properties of the composites. Results show that the composites with a pore size of 100~250μm have a porosity of 75%~90%and the maximum compressive strength of 5.7 MPa. The compressive strength of the composite is greatly improved in comparison with that of HA/CS matrix (4.6 MPa). In the SBF tests, a layer of randomly oriented apatite crystals form on the scaffold surface after sample immersion in SBF. The cell culture experiments show that the osteoblast cells are attached and proliferated on the surface of the composite, which suggests good bioactivity and cellular compatibility of the composite material. It is concluded that the composites have a promising prospect as bone tissue engineering materials.

Effective Cell Seeding and Three-Dimensional Cell Culture for Bone Tissue Engineering

2014 ◽  
Vol 4 (7) ◽  
pp. 573-578 ◽  
Author(s):  
Min Hwei Ng ◽  
Shiplu Roy Chowdhury ◽  
Mahboob Morshed ◽  
Kok Keong Tan ◽  
Guan Huat Tan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Cell Seeding ◽  
Dimensional Cell
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