Designing a novel nanocomposite for bone tissue engineering using electrospun conductive PBAT/polypyrrole as a scaffold to direct nanohydroxyapatite electrodeposition

RSC Advances ◽  
2016 ◽  
Vol 6 (39) ◽  
pp. 32615-32623 ◽  
Author(s):  
Juçara G. de Castro ◽  
Bruno V. M. Rodrigues ◽  
Ritchelli Ricci ◽  
Maíra M. Costa ◽  
André F. C. Ribeiro ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Support Cell

Electrospinning is a well-recognized technique for producing nanostructured fibers with different functionalities, generating materials that are able to support cell adhesion and further proliferation.

scholarly journals 3D-Printed Scaffolds from Alginate/Methyl Cellulose/Trimethyl Chitosan/Silicate Glasses for Bone Tissue Engineering

2021 ◽  
Vol 11 (18) ◽  
pp. 8677
Author(s):  
Maria Fermani ◽  
Varvara Platania ◽  
Rafaela-Maria Kavasi ◽  
Christina Karavasili ◽  
Paola Zgouro ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Methyl Cellulose ◽  
Silicate Glasses ◽  
Trimethyl Chitosan ◽  
Collagen Secretion ◽  
3D Printed ◽  
The Stability

Alginate-based hydrogel inks are commonly used in printing due to their biocompatibility, biodegradation, and cell adhesion. In the present work, 3D printing of hydrogels comprising alginate/methyl cellulose (MC)/trimethyl chitosan (TMC) and silicate glasses was investigated. It was found that TMC increased the stability of the scaffolds after immersion in normal saline solution in comparison with alginate/MC 3D constructs. The stability also remained after the incorporation of pure silicate glasses or bioactive glasses. Immersion in simulated body fluid (SBF) resulted in the formation of hydroxyapatite in all samples. Scanning electron microscopy (SEM) analysis revealed a good cell adhesion of pre-osteoblasts on all scaffold compositions, cell viability assessment displayed a proliferation increase up to seven days in culture, and alkaline phosphatase (ALP) activity was similar in all scaffold compositions without significant differences. Total collagen secretion by the pre-osteoblasts after 7 days in culture was significantly higher in scaffolds containing silicate glasses, demonstrating their ability to promote extracellular matrix formation. In conclusion, 3D-printed porous scaffolds based on alginate/methyl cellulose/TMC are promising candidates for bone tissue engineering applications.

scholarly journals Fabrication and In Vitro Evaluation of 3D Printed Porous Polyetherimide Scaffolds for Bone Tissue Engineering

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Xiongfeng Tang ◽  
Yanguo Qin ◽  
Xinyu Xu ◽  
Deming Guo ◽  
Wenli Ye ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Proliferation ◽  
Cell Adhesion ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
3D Printed

For bone tissue engineering, the porous scaffold should provide a biocompatible environment for cell adhesion, proliferation, and differentiation and match the mechanical properties of native bone tissue. In this work, we fabricated porous polyetherimide (PEI) scaffolds using a three-dimensional (3D) printing system, and the pore size was set as 800 μm. The morphology of 3D PEI scaffolds was characterized by the scanning electron microscope. To investigate the mechanical properties of the 3D PEI scaffold, the compressive mechanical test was performed via an electronic universal testing system. For the in vitro cell experiment, bone marrow stromal cells (BMSCs) were cultured on the surface of the 3D PEI scaffold and PEI slice, and cytotoxicity, cell adhesion, and cell proliferation were detected to verify their biocompatibility. Besides, the alkaline phosphatase staining and Alizarin Red staining were performed on the BMSCs of different samples to evaluate the osteogenic differentiation. Through these studies, we found that the 3D PEI scaffold showed an interconnected porous structure, which was consistent with the design. The elastic modulus of the 3D PEI scaffold (941.33 ± 65.26 MPa) falls in the range of modulus for the native cancellous bone. Moreover, the cell proliferation and morphology on the 3D PEI scaffold were better than those on the PEI slice, which revealed that the porous scaffold has good biocompatibility and that no toxic substances were produced during the progress of high-temperature 3D printing. The osteogenic differentiation level of the 3D PEI scaffold and PEI slice was equal and ordinary. All of these results suggest the 3D printed PEI scaffold would be a potential strategy for bone tissue engineering.

Improved cell adhesion of poly(amino acid) surface by cyclic phosphonate modification for bone tissue engineering

2018 ◽  
Vol 135 (21) ◽  
pp. 46226 ◽  
Author(s):  
Yi Xiong ◽  
Hong Li ◽  
Peng Wang ◽  
Pengzhen Liu ◽  
Yonggang Yan
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Amino Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals Dual delivery of bone morphogenetic protein-2 and basic fibroblast growth factor from nanohydroxyapatite/collagen for bone tissue engineering

2019 ◽  
Vol 62 (1) ◽  
Author(s):  
Yuqian Hu ◽  
Linlin Zheng ◽  
Jinhui Zhang ◽  
Lijuan Lin ◽  
Yue Shen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Growth Factor ◽  
Bone Tissue Engineering ◽  
Fibroblast Growth Factor ◽  
Bone Tissue ◽  
Bone Morphogenetic Protein 2 ◽  
Fibroblast Growth ◽  
Morphogenetic Protein

Abstract Background In bone tissue engineering, the fabrication and biocompatibility of scaffold are crucial. Among many scaffold materials, nanohydroxyapatite (nHAP) and collagen (COL) are chosen as building materials of scaffold. At the same time, growth factors were also used to modify the scaffolds. Methods In this study, blending and freeze drying methods were adopted together in order to build basic fibroblast growth factor (bFGF)-bone morphogenetic protein-2 (BMP-2)-nHAP/COL scaffolds. ELISA was applied to test the release of bFGF and BMP-2 on the scaffold. The flow cytometry was used to identify bone marrow mesenchymal stem cells (BMSCs). Scanning electron microscope was adopted to observe scaffolds and cells morphology. BMSCs were seeded on the scaffolds to test the biological compatibility in vitro. Cells were counted to detect early cell adhesion. Cell counting kit-8 assay was adopted to detect cell proliferation and alkalinephosphatase assay was applied to detect cell activity. Results The characterization of bFGF-BMP-2-nHAP/COL scaffolds meets the requirements of ideal bone tissue engineering scaffolds. BMSCs that were isolated, purified and passaged satisfied the needs of further experiments. The growth status of cells on bFGF-BMP-2-nHAP/COL scaffolds was satisfactory. Cell adhesion was the highest in the bFGF-BMP-2-nHAP/COL scaffolds group. The cell viability and ALP activity of bFGF-BMP-2-nHAP/COL scaffolds group were the highest. Conclusion Taken together, bFGF-BMP-2-nHAP/COL scaffolds have good biocompatibility in vitro and promote adhesion, proliferation, differentiation of BMSCs.

Poly-l-lysine-coated PLGA/poly(amino acid)-modified hydroxyapatite porous scaffolds as efficient tissue engineering scaffolds for cell adhesion, proliferation, and differentiation

2019 ◽  
Vol 43 (25) ◽  
pp. 9989-10002 ◽  
Author(s):  
Shuang Zheng ◽  
Yonghong Guan ◽  
Haichi Yu ◽  
Ge Huang ◽  
Changjun Zheng
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Amino Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Function ◽  
Porous Scaffolds ◽  
Tissue Engineering Scaffolds ◽  
Proliferation And Differentiation

Ideal bone tissue engineering scaffolds should be biocompatible, biodegradable, and mechanically robust and have the ability to regulate cell function.

scholarly journals Porous Silk Fibroin/Cellulose Hydrogels for Bone Tissue Engineering via a Novel Combined Process Based on Sequential Regeneration and Porogen Leaching

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5097
Author(s):  
Dennis Burger ◽  
Marco Beaumont ◽  
Thomas Rosenau ◽  
Yasushi Tamada
Keyword(s):  
Tissue Engineering ◽  
Porous Structure ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Bone Cells ◽  
Support Cell ◽  
Osteogenic Lineage ◽  
New Process ◽  
Vapor Treatment

Scaffolds used for bone tissue engineering need to have a variety of features to accommodate bone cells. The scaffold should mimic natural bone, it should have appropriate mechanical strength, support cell differentiation to the osteogenic lineage, and offer adequate porosity to allow vascularization and bone in-growth. In this work, we aim at developing a new process to fabricate such materials by creating a porous composite material made of silk fibroin and cellulose as a suitable scaffold of bone tissue engineering. Silk fibroin and cellulose are both dissolved together in N,N-dimethylacetamide/LiCl and molded to a porous structure using NaCl powder. The hydrogels are prepared by a sequential regeneration process: cellulose is solidified by water vapor treatment, while the remaining silk fibroin in the hydrogel is insolubilized by methanol, which leads to a cellulose framework structure embedded in a silk fibroin matrix. Finally, the hydrogels are soaked in water to dissolve the NaCl for making a porous structure. The cellulose composition results in improving the mechanical properties for the hydrogels in comparison to the silk fibroin control material. The pore size and porosity are estimated at around 350 µm and 70%, respectively. The hydrogels support the differentiation of MC3T3 cells to osteoblasts and are expected to be a good scaffold for bone tissue engineering.

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