GSH-Activated Light-Up Near-Infrared Fluorescent Probe with High Affinity to αvβ3 Integrin for Precise Early Tumor Identification

2018 ◽  
Vol 10 (37) ◽  
pp. 30994-31007 ◽  
Author(s):  
Zhenwei Yuan ◽  
Lijuan Gui ◽  
Jinrong Zheng ◽  
Yisha Chen ◽  
Sisi Qu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Αvβ3 Integrin ◽  
High Affinity ◽  
Tumor Identification ◽  
Early Tumor

scholarly journals Novel Linear Peptides with High Affinity to αvβ3 Integrin for Precise Tumor Identification

Theranostics ◽  
2017 ◽  
Vol 7 (6) ◽  
pp. 1511-1523 ◽  
Author(s):  
Yi Ma ◽  
Guanhua Ai ◽  
Congying Zhang ◽  
Menglu Zhao ◽  
Xue Dong ◽  
...  
Keyword(s):  
Αvβ3 Integrin ◽  
High Affinity ◽  
Tumor Identification

scholarly journals A High-Affinity Near-Infrared Fluorescent Probe to Target Bombesin Receptors

2014 ◽  
Vol 16 (5) ◽  
pp. 661-669 ◽  
Author(s):  
Ajay Shrivastava ◽  
Haiming Ding ◽  
Shankaran Kothandaraman ◽  
Shu-Huei Wang ◽  
Li Gong ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
High Affinity ◽  
Bombesin Receptors

A supramolecular red to near-infrared fluorescent probe for the detection of drugs in urine

2018 ◽  
Vol 16 (40) ◽  
pp. 7425-7429 ◽  
Author(s):  
Gyan H. Aryal ◽  
Kenneth W. Hunter ◽  
Liming Huang
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Stokes Shift ◽  
Guest Complex ◽  
Large Stokes Shift ◽  
High Affinity ◽  
Host Guest Complex ◽  
Nir Emission ◽  
Perylene Dye

A host–guest complex based on a newly designed and synthesized cationic perylene dye and cucurbit[8]uril exhibits red-NIR emission, high affinity and stability, and large Stokes shift. It can serve as a red-NIR fluorescent displacement probe for the detection of drugs in urine.

Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 758-767
Author(s):  
Vicente Rubio ◽  
Vijaya Iragavarapu ◽  
Maciej J. Stawikowski
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Extinction Coefficient ◽  
Rhodamine B ◽  
Solid Phase ◽  
Total Yield ◽  
Molar Extinction Coefficient ◽  
Αvβ3 Integrin ◽  
Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

A novel near-infrared fluorescent probe based on isophorone for the bioassay of endogenous cysteine

2021 ◽  
Author(s):  
Hong-Bo Liu ◽  
Hai Xu ◽  
Xin Guo ◽  
Jian Xiao ◽  
Zheng-Hong Cai ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Stokes Shift ◽  
Rapid Identification ◽  
Large Stokes Shift

A near-infrared (NIR) fluorescent probe with a large Stokes shift (143 nm) for the rapid identification of Cys over Hcy and GSH in aqueous solution was developed.

A mitochondria-targeted near-infrared fluorescent probe for imaging viscosity in living cells and a diabetic mice model

2021 ◽  
Author(s):  
Bochao Chen ◽  
Shumei Mao ◽  
Yanyan Sun ◽  
Liyuan Sun ◽  
Ning Ding ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Diabetic Mouse ◽  
Living Cells ◽  
Pancreatic Tissue ◽  
Diabetic Mice ◽  
Mice Model ◽  
Viscosity Changes

A mitochondria-targeted near-infrared fluorescent probe NIR-V with 700 nm emission was designed to monitor cell viscosity changes, which was applied to detect the intracellular viscosity and imagine pancreatic tissue in diabetic mouse model.

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