Collagen Fibril-Assembled Skin-Simulated Membrane for Continuous Molecular Separation

Author(s):  
Jian Li ◽  
Pei Xiao ◽  
Yilin Xu ◽  
Liangliang Dong ◽  
Zhenyu Wang ◽  
...  
Author(s):  
Barry F. King ◽  
Grete N. Fry

The amnion surrounding the mammalian embryo consists of the amniotic epithelium facing the amniotic cavity, a layer of extraembryonic mesoderm bordering the exocoelom and an intervening layer of extracellular matrix (Fig. 1). During gestation the amnion expands remarkably to acommodate the rapidly growing embryo. In this study we have examined the process of collagen fibril formation in the developing amnion of the rhesus monkey between 20 and 60 days of gestation.Most cytological evidence of collagen fibril formation was observed in association with the extraembryonic mesodermal cells rather than the amniotic epithelium. The mesodermal cells h ad abundant cisternae of rough endoplasmic reticulum and a prominent Golgi apparatus. Elongated secretory vacuoles were associated with the Golgi apparatus and often contained parallel aggregates of fine filaments (Fig. 2). In some secretory vacuoles, periodic densities also were observed. Some striated collagen fibrils were observed in an apparent intracellular location in long, membrane-limited compartments (Fig. 3). Still other striated fibrils were observed in dense bodies, presumably lysosomes (Fig. 4).


2017 ◽  
Vol 68 (2) ◽  
pp. 203-209
Author(s):  
Hussam Nadum Abdalraheem Al Ani ◽  
Anca Maria Cimbru ◽  
Corneliu Trisca-Rusu ◽  
Szidonia Katalin Tanczos ◽  
Adriana Cuciureanu ◽  
...  

This paper illustrates the possibility of producing iono-molecular separations using ionic colloidal ultrafiltration membrane of polysulfone synthetic solutions of cupric ions and nitro phenols through ultrafiltration assisted by polymeric nanoparticle composites based on polysulfone. In the present work, in order to reduce the operating pressure and increase the flow of water we are using the process of ultrafiltration through a polysulfone membrane in N-methylpyrrolidone 10% prepared by coagulation in isopropanol. The nanoparticles needed in colloidal ultrafiltration had been obtained through the immersion technique of precipitation of a solution of 5% PSf in N-methyl pyrrolidone containing 3% aniline in lower alcohols: methanol, ethanol, and isopropanol, followed by the oxidation of the remaining aniline in a solution of 10% hydrochloric acid and ammonium persulfate. The Nanoparticles of polysulfone (NP-PSf) and The three obtained variants of nanoparticles composites (NP-PSf-PANI) were morphologically (SEM) and (AFM), structurally and compositionally (FTIR) characterized and the results show that nanoparticles polysulfone have a much lower range than the composites. The Possibility of copper complexation by both nitrophenols, and by nanoparticle surface probably lead to the formation of more stable aggregates in the supply, which can sufficiently justify the increased retention. The Retentions of the chemical species in question use in all the tests made the same series:R NP-PSf-PANI-M] R NP-PSf-PANI-E] R NP-PSf-PANI-P] R NP-PSf


2018 ◽  
Vol 69 (7) ◽  
pp. 1603-1607
Author(s):  
Ion Spiridon Din ◽  
Anca Maria Cimbru ◽  
Abbas Abdul Kadhim Klaif Rikabi ◽  
Szidonia Katalin Tanczos ◽  
Simona Ticu (Cotorcea) ◽  
...  

The importance of removing and / or separating nitro phenols from aqueous solutions through membranes is substantiated by the multitude of recent research in the field, which broadly justifies both the economic and ecological reasons of such an approach. The present paper outlines the results of the transfer of nitro phenols through a membrane system made up of PPET impregnated polypropylene capillaries (PP) impregnated with sulfonate polyetheretherketone (SPEEK). The experiments were carried out in a PP-SPEEK capillary membrane module, with a useful size of 1 m2. Determinations made by using a 4 L / min flow rate source at a 5 mg / l nitrophenol concentration and pH 5 or pH 7, and the pH 12 receiving phase and a flow rate of 0.3 L / min, revealed that o- and p-nitrophenol were transferred much faster than m-nitrophenol (the flux is nearly double); the source phase of the system is concentrated in m-nitrophenol, and the receptor phase in o- and p-nitro phenols; the transfer data correlates with the higher water solubility of m-nitrophenol; mono nitro phenols transfer much faster than di nitrophenol, but both the mono and di nitrophenol streams decrease over time; after 4-5 hours of work, the mono nitrophenol concentration triples in the receiving phase, while the 2,4-dinitrophenol concentration doubles in the source phase.


1993 ◽  
Vol 268 (26) ◽  
pp. 19826-19832
Author(s):  
J.R. MacBeath ◽  
D.R. Shackleton ◽  
D.J. Hulmes

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Susyn Joan Kelly ◽  
Lizette duPlessis ◽  
John Soley ◽  
Frazer Noble ◽  
Hannah Carolyn Wells ◽  
...  

Abstract Objective Small angle X-ray scattering (SAXS) analysis is a sensitive way of determining the ultrastructure of collagen in tissues. Little is known about how parameters measured by SAXS are affected by preservatives commonly used to prevent autolysis. We determined the effects of formalin, glutaraldehyde, Triton X and saline on measurements of fibril diameter, fibril diameter distribution, and D-spacing of corneal collagen using SAXS analysis. Results Compared to sections of sheep and cats’ corneas stored frozen as controls, those preserved in 5% glutaraldehyde and 10% formalin had significantly larger mean collagen fibril diameters, increased fibril diameter distribution and decreased D-spacing. Sections of corneas preserved in Triton X had significantly increased collagen fibril diameters and decreased fibril diameter distribution. Those preserved in 0.9% saline had significantly increased mean collagen fibril diameters and decreased diameter distributions. Subjectively, the corneas preserved in 5% glutaraldehyde and 10% formalin maintained their transparency but those in Triton X and 0.9% saline became opaque. Subjective morphological assessment of transmission electron microscope images of corneas supported the SAXS data. Workers using SAXS analysis to characterize collagen should be alerted to changes that can be introduced by common preservatives in which their samples may have been stored.


ACS Nano ◽  
2021 ◽  
Author(s):  
Ji Hoon Kim ◽  
Gyeong Seok Park ◽  
Yong-Jae Kim ◽  
Eunji Choi ◽  
Junhyeok Kang ◽  
...  

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