Tetrahedral Framework Nucleic Acids Loaded with Aptamer AS1411 for siRNA Delivery and Gene Silencing in Malignant Melanoma

2021 ◽  
Vol 13 (5) ◽  
pp. 6109-6118 ◽  
Author(s):  
Dexuan Xiao ◽  
Yanjing Li ◽  
Taoran Tian ◽  
Tianxu Zhang ◽  
Sirong Shi ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Nucleic Acids ◽  
Gene Silencing ◽  
Sirna Delivery ◽  
Tetrahedral Framework

scholarly journals Development of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage

2018 ◽  
Author(s):  
Daniel Clarke ◽  
Adi Idris ◽  
Nigel AJ McMillan
Keyword(s):  
Particle Size ◽  
Nucleic Acids ◽  
Gene Silencing ◽  
Room Temperature ◽  
Sirna Delivery ◽  
Long Term Stability ◽  
Freeze Dried ◽  
Lipid Particles ◽  
End Products

ABSTRACTLiposomes are versatile and well-proven as a means to deliver nucleic acids into cells. Most of the formulation procedures used are labour intensive and result in unstable end products. We have previously reported on the development of a simple, yet efficient, hydration-of-freeze-dried-matrix (HFDM) method to entrap siRNA within lipid particles. Here we show that the particles are stable up to 12 months after storage room temperature (RT), 4°C or - 20°C. While RT storage results in changes in particle size and polydispersity, gene silencing of all particles was similar to freshly prepared particles following storage for 3, 6, 9 or 12 months at all temperatures. This is the first report of such long-term stability in siRNA-loaded liposomes.

Visible quantum-dot-based targeted siRNA delivery for HIF-1α gene silencing

2014 ◽  
Vol 24 (5) ◽  
pp. 445-451 ◽  
Author(s):  
HongYan Zhu ◽  
JingPing Zhu ◽  
AiMei Xie ◽  
Yong Lin ◽  
BeiBei Zhang ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Gene Silencing ◽  
Sirna Delivery

scholarly journals In vivo gene silencing following non-invasive siRNA delivery into the skin using a novel topical formulation

2014 ◽  
Vol 196 ◽  
pp. 355-362 ◽  
Author(s):  
Vikas Hegde ◽  
Robyn P. Hickerson ◽  
Sitheswaran Nainamalai ◽  
Paul A. Campbell ◽  
Frances J.D. Smith ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Sirna Delivery ◽  
Topical Formulation ◽  
Non Invasive

Biocompatible, chimeric peptide-condensed supramolecular nanoparticles for tumor cell-specific siRNA delivery and gene silencing

2014 ◽  
Vol 50 (58) ◽  
pp. 7806-7809 ◽  
Author(s):  
Hangxiang Wang ◽  
Wei Chen ◽  
Haiyang Xie ◽  
Xuyong Wei ◽  
Shengyong Yin ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Gene Silencing ◽  
Delivery System ◽  
Self Assembly ◽  
Sirna Delivery ◽  
Single Step ◽  
Chimeric Peptide ◽  
Supramolecular Nanoparticles ◽  
Sirna Delivery System

A practical and tumor cell-specific siRNA delivery system was developedviasingle-step self-assembly of an arginine-rich chimeric peptide with siRNA.

An injectable paste of calcium phosphate nanorods, functionalized with nucleic acids, for cell transfection and gene silencing

2010 ◽  
Vol 20 (29) ◽  
pp. 6144 ◽  
Author(s):  
J. Klesing ◽  
S. Chernousova ◽  
A. Kovtun ◽  
S. Neumann ◽  
L. Ruiz ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Nucleic Acids ◽  
Gene Silencing ◽  
Cell Transfection

scholarly journals WRAP-based nanoparticles for siRNA delivery: a SAR study and a comparison with lipid-based transfection reagents

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Karidia Konate ◽  
Emilie Josse ◽  
Milana Tasic ◽  
Karima Redjatti ◽  
Gudrun Aldrian ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Gene Silencing ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Sirna Delivery ◽  
Cell Penetrating Peptides ◽  
Sirna Transfection ◽  
Arginine Residues ◽  
Sar Study

AbstractRecently, we designed novel amphipathic cell-penetrating peptides, called WRAP, able to transfer efficiently siRNA molecules into cells. In order to gain more information about the relationship between amino acid composition, nanoparticle formation and cellular internalization of these peptides composed of only three amino acids (leucine, arginine and tryptophan), we performed a structure–activity relationship (SAR) study. First, we compared our WRAP1 and WRAP5 peptides with the C6M1 peptide also composed of the same three amino acids and showing similar behaviors in siRNA transfection. Afterwards, to further define the main determinants in the WRAP activity, we synthesized 13 new WRAP analogues harboring different modifications like the number and location of leucine and arginine residues, the relative location of tryptophan residues, as well as the role of the α-helix formation upon proline insertions within the native WRAP sequence. After having compared the ability of these peptides to form peptide-based nanoparticles (PBNs) using different biophysical methods and to induce a targeted gene silencing in cells, we established the main sequential requirements of the amino acid composition of the WRAP peptide. In addition, upon measuring the WRAP-based siRNA transfection ability into cells compared to several non-peptide transfection agents available on the markets, we confirmed that WRAP peptides induced an equivalent level of targeted gene silencing but in most of the cases with lower cell toxicity as clearly shown in clonogenic assays.

scholarly journals Diblock Copolymer Hydrophobicity Facilitates Efficient Gene Silencing and Cytocompatible Nanoparticle-Mediated siRNA Delivery to Musculoskeletal Cell Types

2017 ◽  
Vol 18 (11) ◽  
pp. 3753-3765 ◽  
Author(s):  
Dominic W. Malcolm ◽  
Margaret A. T. Freeberg ◽  
Yuchen Wang ◽  
Kenneth R. Sims ◽  
Hani A. Awad ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Diblock Copolymer ◽  
Sirna Delivery ◽  
Cell Types ◽  
Efficient Gene
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