scholarly journals Development of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage

2018 ◽  
Author(s):  
Daniel Clarke ◽  
Adi Idris ◽  
Nigel AJ McMillan
Keyword(s):  
Particle Size ◽  
Nucleic Acids ◽  
Gene Silencing ◽  
Room Temperature ◽  
Sirna Delivery ◽  
Long Term Stability ◽  
Freeze Dried ◽  
Lipid Particles ◽  
End Products

ABSTRACTLiposomes are versatile and well-proven as a means to deliver nucleic acids into cells. Most of the formulation procedures used are labour intensive and result in unstable end products. We have previously reported on the development of a simple, yet efficient, hydration-of-freeze-dried-matrix (HFDM) method to entrap siRNA within lipid particles. Here we show that the particles are stable up to 12 months after storage room temperature (RT), 4°C or - 20°C. While RT storage results in changes in particle size and polydispersity, gene silencing of all particles was similar to freshly prepared particles following storage for 3, 6, 9 or 12 months at all temperatures. This is the first report of such long-term stability in siRNA-loaded liposomes.

Long-Term Preservation at Room Temperature of Freeze-Dried Human Tumor Samples Dedicated to Nucleic Acids Analyses

2008 ◽  
Vol 6 (3) ◽  
pp. 191-198 ◽  
Author(s):  
Christophe Leboeuf ◽  
Philippe Ratajczak ◽  
Wei-Li Zhao ◽  
Louis François Plassa ◽  
Magali Court ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Room Temperature ◽  
Human Tumor ◽  
Freeze Dried ◽  
Long Term Preservation

The Stability of the WHO Reference Thromboplastin NIBS & C 67/40

1979 ◽  
Vol 42 (04) ◽  
pp. 1135-1140 ◽  
Author(s):  
G I C Ingram
Keyword(s):  
Human Brain ◽  
Collaborative Study ◽  
Calibration Constant ◽  
Long Term Stability ◽  
Freeze Dried ◽  
Show Evidence ◽  
Human Material ◽  
Reference Preparation ◽  
The Stability

SummaryThe International Reference Preparation of human brain thromboplastin coded 67/40 has been thought to show evidence of instability. The evidence is discussed and is not thought to be strong; but it is suggested that it would be wise to replace 67/40 with a new preparation of human brain, both for this reason and because 67/40 is in a form (like Thrombotest) in which few workers seem to use human brain. A �plain� preparation would be more appropriate; and a freeze-dried sample of BCT is recommended as the successor preparation. The opportunity should be taken also to replace the corresponding ox and rabbit preparations. In the collaborative study which would be required it would then be desirable to test in parallel the three old and the three new preparations. The relative sensitivities of the old preparations could be compared with those found in earlier studies to obtain further evidence on the stability of 67/40; if stability were confirmed, the new preparations should be calibrated against it, but if not, the new human material should receive a calibration constant of 1.0 and the new ox and rabbit materials calibrated against that.The types of evidence available for monitoring the long-term stability of a thromboplastin are discussed.

Water repellent room temperature vulcanized silicone for enhancing the long-term stability of perovskite solar cells

Solar Energy ◽  
2021 ◽  
Vol 218 ◽  
pp. 28-34
Author(s):  
Mahmoud Samadpour ◽  
Mahsa Heydari ◽  
Mahdi Mohammadi ◽  
Parisa Parand ◽  
Nima Taghavinia
Keyword(s):  
Solar Cells ◽  
Room Temperature ◽  
Perovskite Solar Cells ◽  
Water Repellent ◽  
Term Stability ◽  
Long Term Stability

Development of a 300°C capable SiC based operational amplifier

2010 ◽  
Vol 2010 (HITEC) ◽  
pp. 000305-000309 ◽  
Author(s):  
Vinayak Tilak ◽  
Cheng-Po Chen ◽  
Peter Losee ◽  
Emad Andarawis ◽  
Zachary Stum
Keyword(s):  
Silicon Carbide ◽  
High Temperature ◽  
Operational Amplifier ◽  
Room Temperature ◽  
Open Loop ◽  
Common Source ◽  
Loop Gain ◽  
Long Term Stability ◽  
Silicon Silicon

Silicon carbide based ICs have the potential to operate at temperatures exceeding that of conventional semiconductors such as silicon. Silicon carbide (SiC) based MOSFETs and ICs were fabricated and measured at room temperature and 300°C. A common source amplifier was fabricated and tested at room temperature and high temperature. The gain at room temperature and high temperature was 7.6 and 6.8 respectively. A SiC MOSFET based operational amplifier was also fabricated and tested at room temperature and 300°C. The small signal open loop gain at 1kHz was 60 dB at room temperature and 57 dB at 300°C. Long term stability testing at 300°C of the MOSFET and common source amplifiers showed very little drift.

NANOEMULSIONS BASED ON LIPOIC ACID DERIVATIVES WITH VARIOUS ALCOHOLS: PRODUCTION AND INFLUENCE ON FUNCTIONAL ACTIVITY OF PLATELETS

2020 ◽  
pp. 461-464
Author(s):  
A. Sinebryukhova ◽  
A. Shipelova ◽  
E. Darnotuk ◽  
A. Chekanov ◽  
O. Baranova ◽  
...  
Keyword(s):  
Particle Size ◽  
Arachidonic Acid ◽  
Lipoic Acid ◽  
Encapsulation Efficiency ◽  
Room Temperature ◽  
Optimal Conditions ◽  
Pluronic F68 ◽  
Long Term Storage ◽  
Term Storage

The optimal conditions were selected for obtaining homogeneous nanoemulsions (NE) of lipoic acid conjugates (LA-conjugates) based on Pluronic F68 (1,8%) with a particle size not exceeding 400 nm, characterized by 97±2% encapsulation efficiency of substances in nanoparticles (NP). A heterogeneous NE (polydispersity index, PDI>0,3) with the derivative of LA and myo-inositol based on phosphatidylcholine (PC, C = 3 mg/ml) was also obtained consisting of 2 particle fractions: 20–70 nm (27%) and 122–212 nm (73%). The obtained NEs with LA-conjugates based on Pluronic F68 and PC were stable during long-term storage (more than 12 months) at room temperature. The effect of the obtained NEs of LA-conjugates on platelet aggregation (Pt) caused by arachidonic acid (AA) was determined, and a mechanism of their action was proposed.

scholarly journals A Room Temperature Gas Sensor Based on Sulfonated SWCNTs for the Detection of NO and NO2

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1116 ◽  
Author(s):  
Eusebiu Ionete ◽  
Stefan Spiridon ◽  
Bogdan Monea ◽  
Elena Stratulat
Keyword(s):  
Room Temperature ◽  
Gas Sensing ◽  
Electrical Response ◽  
Long Term Stability ◽  
Sensing Applications ◽  
Specific Configuration ◽  
P Type ◽  
Walled Carbon Nanotubes ◽  
Type Response

The electrical response of sulfonated single-walled carbon nanotubes (SWCNTs) to NO and NO2, for gas sensing applications, at room temperature, is reported in this work. A specific configuration based on SWCNT deposition between double pair configuration gold electrodes, supported on a substrate, was considered for the sensing device; employed characterization technique where FTIR and SEM. The experimental results showed a p-type response of the sulfonated SWCNTs, with decrease in resistance, under exposure to NO gas (40–200 ppb) and NO2 (40–200 ppb). Also, the sensor responses to successive exposures at NO2 800 ppb together with investigation of long term stability, at 485 ppb for NO, are reported. The reaction mechanism in case of NO and NO2 detection with sulfonated SWCNTs is presented.

Effects of La2O3 content and particle size on the long-term stability and thermal cycling property of La2O3-dispersed SUS430 alloys for SOFC interconnect materials

2017 ◽  
Vol 23 (6) ◽  
pp. 1250-1256 ◽  
Author(s):  
Jung-Won Lee ◽  
Muhammad Taqi Mehran ◽  
Rak-Hyun Song ◽  
Seung-Bok Lee ◽  
Jong-Won Lee ◽  
...  
Keyword(s):  
Particle Size ◽  
Thermal Cycling ◽  
Term Stability ◽  
Long Term Stability

Lanthanide Refractory Semiconductors Based on the Th3P4 Structure

1987 ◽  
Vol 97 ◽  
Author(s):  
K. A. Gschneidner ◽  
J. F. Nakahara ◽  
B. J. Beaudry ◽  
T. Takeshita ◽  
Ames Laboratory
Keyword(s):  
Electrical Resistivity ◽  
Thermal Properties ◽  
Seebeck Coefficient ◽  
Thermoelectric Properties ◽  
Room Temperature ◽  
Type Structure ◽  
Term Stability ◽  
Long Term Stability ◽  
Phase Relationships

ABSTRACTThe phase relationships and the important structural, electrical and thermal properties of the R3X4-R2X3 (where R = lanthanides and X = S, Se and Te) phases having the Th3P4 -type structure are reviewed. The room temperature electrical resistivity and Seebeck coefficient of these materials are independent of R and only slightly dependent on X, but critically dependent on the X:R ratio. The long term stability of these phases is also reviewed. Although these materials have good thermoelectric properties there are some problems which need to be solved before these phases can be utilized in thermoelectric devices. These problems include long term stability, higher than desirable thermal conductivities, and low electron mobilities.

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