scholarly journals Photo-cross-linked Gelatin Glycidyl Methacrylate/N-Vinylpyrrolidone Copolymeric Hydrogel with Tunable Mechanical Properties for Ocular Tissue Engineering Applications

2021 ◽  
Author(s):  
Sina Sharifi ◽  
Hannah Sharifi ◽  
Ali Akbari ◽  
Darrell Koza ◽  
Claes H. Dohlman ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Glycidyl Methacrylate ◽  
Ocular Tissue ◽  
Engineering Applications ◽  
Tunable Mechanical Properties

An engineered cell-laden adhesive hydrogel promotes craniofacial bone tissue regeneration in rats

2020 ◽  
Vol 12 (534) ◽  
pp. eaay6853 ◽  
Author(s):  
Mohammad Mahdi Hasani-Sadrabadi ◽  
Patricia Sarrion ◽  
Sevda Pouraghaei ◽  
Yee Chau ◽  
Sahar Ansari ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Tissue Regeneration ◽  
Engineering Applications ◽  
Craniofacial Bone ◽  
Oral Environment ◽  
Tunable Mechanical Properties ◽  
Craniofacial Tissue

Cell-laden hydrogels are widely used in tissue engineering and regenerative medicine. However, many of these hydrogels are not optimized for use in the oral environment, where they are exposed to blood and saliva. To address these challenges, we engineered an alginate-based adhesive, photocrosslinkable, and osteoconductive hydrogel biomaterial (AdhHG) with tunable mechanical properties. The engineered hydrogel was used as an injectable mesenchymal stem cell (MSC) delivery vehicle for craniofacial bone tissue engineering applications. Subcutaneous implantation in mice confirmed the biodegradability, biocompatibility, and osteoconductivity of the hydrogel. In a well-established rat peri-implantitis model, application of the adhesive hydrogel encapsulating gingival mesenchymal stem cells (GMSCs) resulted in complete bone regeneration around ailing dental implants with peri-implant bone loss. Together, we have developed a distinct bioinspired adhesive hydrogel with tunable mechanical properties and biodegradability that effectively delivers patient-derived dental-derived MSCs. The hydrogel is photocrosslinkable and, due to the presence of MSC aggregates and hydroxyapatite microparticles, promotes bone regeneration for craniofacial tissue engineering applications.

Synthesis and characterization of poly(glycerol sebacate)-based elastomeric copolyesters for tissue engineering applications

2016 ◽  
Vol 7 (14) ◽  
pp. 2553-2564 ◽  
Author(s):  
Yating Jia ◽  
Weizhong Wang ◽  
Xiaojun Zhou ◽  
Wei Nie ◽  
Liang Chen ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Water Uptake ◽  
Synthesis And Characterization ◽  
Uptake Capacity ◽  
Engineering Applications ◽  
Water Uptake Capacity

A poly(glycerol sebacate)-based elastomeric copolyesters with improved mechanical properties and higher water uptake capacity.

scholarly journals Alginate/Gelatin Hydrogels Reinforced with TiO2 and β-TCP Fabricated by Microextrusion-based Printing for Tissue Regeneration

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 457 ◽  
Author(s):  
Rodrigo Urruela-Barrios ◽  
Erick Ramírez-Cedillo ◽  
A. Díaz de León ◽  
Alejandro Alvarez ◽  
Wendy Ortega-Lara
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Average Pore Size ◽  
Three Dimensional ◽  
Engineering Applications ◽  
Average Pore ◽  
Viscous Deformation ◽  
Freeze Dried ◽  
3D Printed

Three-dimensional (3D) printing technologies have become an attractive manufacturing process to fabricate scaffolds in tissue engineering. Recent research has focused on the fabrication of alginate complex shaped structures that closely mimic biological organs or tissues. Alginates can be effectively manufactured into porous three-dimensional networks for tissue engineering applications. However, the structure, mechanical properties, and shape fidelity of 3D-printed alginate hydrogels used for preparing tissue-engineered scaffolds is difficult to control. In this work, the use of alginate/gelatin hydrogels reinforced with TiO2 and β-tricalcium phosphate was studied to tailor the mechanical properties of 3D-printed hydrogels. The hydrogels reinforced with TiO2 and β-TCP showed enhanced mechanical properties up to 20 MPa of elastic modulus. Furthermore, the pores of the crosslinked printed structures were measured with an average pore size of 200 μm. Additionally, it was found that as more layers of the design were printed, there was an increase of the line width of the bottom layers due to its viscous deformation. Shrinkage of the design when the hydrogel is crosslinked and freeze dried was also measured and found to be up to 27% from the printed design. Overall, the proposed approach enabled fabrication of 3D-printed alginate scaffolds with adequate physical properties for tissue engineering applications.

Cellular Cross-linking of Peptide Modified Hydrogels

2004 ◽  
Vol 127 (2) ◽  
pp. 220-228 ◽  
Author(s):  
Jeanie L. Drury ◽  
Tanyarut Boontheekul ◽  
David J. Mooney
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Density ◽  
Tensile Tests ◽  
Cross Linking ◽  
Engineering Applications ◽  
C2c12 Myoblasts ◽  
Polymer Systems ◽  
Peptide Modification ◽  
Adhesive Interactions

Peptide modification of hydrogel-forming materials is being widely explored as a means to regulate the phenotype of cells immobilized within the gels. Alternatively, we hypothesized that the adhesive interactions between cells and peptides coupled to the gel-forming materials would also enhance the overall mechanical properties of the gels. To test this hypothesis, alginate polymers were modified with RGDSP-containing peptides and the resultant polymer was used to encapsulate C2C12 myoblasts. The mechanical properties of these gels were then assessed as a function of both peptide and cell density using compression and tensile tests. Overall, it was found that above a critical peptide and cell density, encapsulated myoblasts were able to provide additional mechanical integrity to hydrogels composed of peptide-modified alginate. This occurred presumably by means of cell-peptide cross-linking of the alginate polymers, in addition to the usual Ca++ cross-linking. These results are potentially applicable to other polymer systems and important for a range of tissue engineering applications.

scholarly journals Compressed collagen constructs with optimized mechanical properties and cell interactions for tissue engineering applications

2018 ◽  
Vol 108 ◽  
pp. 158-166 ◽  
Author(s):  
Fatemeh Ajalloueian ◽  
Nikolaos Nikogeorgos ◽  
Ali Ajalloueian ◽  
Magdalena Fossum ◽  
Seunghwan Lee ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Interactions ◽  
Engineering Applications
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