SERS Imaging of Mesenchymal Stromal Cell Differentiation

2021 ◽  
Author(s):  
Adrianna Milewska ◽  
Olafur E. Sigurjonsson ◽  
Kristjan Leosson
Keyword(s):  
Cell Differentiation ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Sers Imaging

scholarly journals Asporin Restricts Mesenchymal Stromal Cell Differentiation, Alters the Tumor Microenvironment, and Drives Metastatic Progression

2019 ◽  
Vol 79 (14) ◽  
pp. 3636-3650 ◽  
Author(s):  
Robert M. Hughes ◽  
Brian W. Simons ◽  
Hamda Khan ◽  
Rebecca Miller ◽  
Valentina Kugler ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Tumor Microenvironment ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Metastatic Progression

Monitoring human mesenchymal stromal cell differentiation by electrochemical impedance sensing

Cytotherapy ◽  
2011 ◽  
Vol 13 (9) ◽  
pp. 1074-1089 ◽  
Author(s):  
Michael Angstmann ◽  
Irena Brinkmann ◽  
Karen Bieback ◽  
Dirk Breitkreutz ◽  
Christian Maercker
Keyword(s):  
Cell Differentiation ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Electrochemical Impedance ◽  
Impedance Sensing

scholarly journals Monitoring live human mesenchymal stromal cell differentiation and subsequent selection using fluorescent RNA-based probes

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Bojun Li ◽  
Ursula Menzel ◽  
Claudia Loebel ◽  
Hagen Schmal ◽  
Mauro Alini ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Mrna Expression ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Early Time ◽  
Live Cells ◽  
Osteogenic Potential ◽  
Homogeneous Cell ◽  
The Individual ◽  
Multiple Samples

Abstract Investigating mesenchymal stromal cell differentiation requires time and multiple samples due to destructive endpoint assays. Osteogenesis of human bone marrow derived mesenchymal stromal cells (hBMSCs) has been widely studied for bone tissue engineering. Recent studies show that the osteogenic differentiation of hBMSCs can be assessed by quantifying the ratio of two important transcription factors (Runx2/Sox9). We demonstrate a method to observe mRNA expression of two genes in individual live cells using fluorescent probes specific for Runx2 and Sox9 mRNA. The changes of mRNA expression in cells can be observed in a non-destructive manner. In addition, the osteogenic hBMSCs can be prospectively identified and obtained based on the relative intracellular fluorescence of Sox9 in relation to Runx2 using fluorescence activated cell sorting. Relatively homogeneous cell populations with high osteogenic potential can be isolated from the original heterogeneous osteogenically induced hBMSCs within the first week of induction. This offers a more detailed analysis of the effectiveness of new therapeutics both at the individual cell level and the response of the population as a whole. By identifying and isolating differentiating cells at early time points, prospective analysis of differentiation is also possible, which will lead to a greater understanding of MSC differentiation.

scholarly journals Influence of PCL molecular weight on mesenchymal stromal cell differentiation

RSC Advances ◽  
2015 ◽  
Vol 5 (67) ◽  
pp. 54510-54516 ◽  
Author(s):  
W. J. Hendrikson ◽  
J. Rouwkema ◽  
C. A. van Blitterswijk ◽  
L. Moroni
Keyword(s):  
Molecular Weight ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Cell Activity ◽  
Additional Parameter ◽  
Polymer Molecular Weight ◽  
Stem Cell Activity ◽  
Skeletal Regeneration

The molecular weight of polycaprolactone was varied to investigate its effect on stem cell activity. Results showed that polymer molecular weight is an additional parameter to consider when designing scaffolds for skeletal regeneration.

SAT0548 Impact of Glucocorticoids on Mesenchymal Stromal Cell Differentiation - Can Hypoxia Counteract?

2014 ◽  
Vol 73 (Suppl 2) ◽  
pp. 789.2-789
Author(s):  
A. Lang ◽  
K. Schmidt-Bleek ◽  
M. Jackstadt ◽  
T. Gaber ◽  
G. Duda ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell
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