scholarly journals Influence of PCL molecular weight on mesenchymal stromal cell differentiation

RSC Advances ◽  
2015 ◽  
Vol 5 (67) ◽  
pp. 54510-54516 ◽  
Author(s):  
W. J. Hendrikson ◽  
J. Rouwkema ◽  
C. A. van Blitterswijk ◽  
L. Moroni
Keyword(s):  
Molecular Weight ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Cell Activity ◽  
Additional Parameter ◽  
Polymer Molecular Weight ◽  
Stem Cell Activity ◽  
Skeletal Regeneration

The molecular weight of polycaprolactone was varied to investigate its effect on stem cell activity. Results showed that polymer molecular weight is an additional parameter to consider when designing scaffolds for skeletal regeneration.

scholarly journals Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6

2008 ◽  
Vol 87 (3) ◽  
pp. 239-245 ◽  
Author(s):  
Natsumi Shimizu ◽  
Shinichi Noda ◽  
Kazufumi Katayama ◽  
Hitoshi Ichikawa ◽  
Hiroaki Kodama ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Line ◽  
Hematopoietic Stem Cell ◽  
Stromal Cell ◽  
Cell Activity ◽  
Hematopoietic Stem ◽  
Stem Cell Activity ◽  
Stromal Cell Line

scholarly journals In Vitro Maintenance of Highly Purified, Transplantable Hematopoietic Stem Cells

Blood ◽  
1997 ◽  
Vol 89 (12) ◽  
pp. 4337-4347 ◽  
Author(s):  
Kateri A. Moore ◽  
Hideo Ema ◽  
Ihor R. Lemischka
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Lines ◽  
Progenitor Cell ◽  
Stromal Cell ◽  
Fetal Liver ◽  
Cell Activity ◽  
Hematopoietic Stem ◽  
Stem Cell Activity

Abstract The cellular and molecular mechanisms that regulate the most primitive hematopoietic stem cell are not well understood. We have undertaken a systematic dissection of the complex hematopoietic microenvironment to define some of these mechanisms. An extensive panel of immortalized stromal cell lines from murine fetal liver were established and characterized. Collectively, these cell lines display extensive heterogeneity in their in vitro hematopoietic supportive capacity. In the current studies, we describe a long-term in vitro culture system using a single stromal cell clone (AFT024) that qualitatively and quantitatively supports transplantable stem cell activity present in highly purified populations. We show multilineage reconstitution in mice that received the equivalent of as few as 100 purified bone marrow and fetal liver stem cells cultured for 4 to 7 weeks on AFT024. The cultured stem cells meet all functional criteria currently ascribed to the most primitive stem cell population. The levels of stem cell activity present after 5 weeks of coculture with AFT024 far exceed those present in short-term cytokine-supported cultures. In addition, maintenance of input levels of transplantable stem cell activity is accompanied by expansion of other classes of stem/progenitor cells. This suggests that the stem/progenitor cell population is actively proliferating in culture and that the AFT024 cell line provides a milieu that stimulates progenitor cell proliferation while maintaining in vivo repopulating activity.

scholarly journals Spatial Coordination between Stem Cell Activity and Cell Differentiation in the Root Meristem

2013 ◽  
Vol 26 (4) ◽  
pp. 405-415 ◽  
Author(s):  
Laila Moubayidin ◽  
Riccardo Di Mambro ◽  
Rosangela Sozzani ◽  
Elena Pacifici ◽  
Elena Salvi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Root Meristem ◽  
Cell Activity ◽  
Spatial Coordination ◽  
Stem Cell Activity

scholarly journals Stromal cell-dependent ex vivo expansion of human cord blood progenitors and augmentation of transplantable stem cell activity

2000 ◽  
Vol 26 (8) ◽  
pp. 837-844 ◽  
Author(s):  
M Kanai ◽  
F Hirayama ◽  
M Yamaguchi ◽  
J Ohkawara ◽  
N Sato ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Stromal Cell ◽  
Ex Vivo ◽  
Cell Activity ◽  
Ex Vivo Expansion ◽  
Human Cord Blood ◽  
Stem Cell Activity
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