scholarly journals Monitoring live human mesenchymal stromal cell differentiation and subsequent selection using fluorescent RNA-based probes

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Bojun Li ◽  
Ursula Menzel ◽  
Claudia Loebel ◽  
Hagen Schmal ◽  
Mauro Alini ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Mrna Expression ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Early Time ◽  
Live Cells ◽  
Osteogenic Potential ◽  
Homogeneous Cell ◽  
The Individual ◽  
Multiple Samples

Abstract Investigating mesenchymal stromal cell differentiation requires time and multiple samples due to destructive endpoint assays. Osteogenesis of human bone marrow derived mesenchymal stromal cells (hBMSCs) has been widely studied for bone tissue engineering. Recent studies show that the osteogenic differentiation of hBMSCs can be assessed by quantifying the ratio of two important transcription factors (Runx2/Sox9). We demonstrate a method to observe mRNA expression of two genes in individual live cells using fluorescent probes specific for Runx2 and Sox9 mRNA. The changes of mRNA expression in cells can be observed in a non-destructive manner. In addition, the osteogenic hBMSCs can be prospectively identified and obtained based on the relative intracellular fluorescence of Sox9 in relation to Runx2 using fluorescence activated cell sorting. Relatively homogeneous cell populations with high osteogenic potential can be isolated from the original heterogeneous osteogenically induced hBMSCs within the first week of induction. This offers a more detailed analysis of the effectiveness of new therapeutics both at the individual cell level and the response of the population as a whole. By identifying and isolating differentiating cells at early time points, prospective analysis of differentiation is also possible, which will lead to a greater understanding of MSC differentiation.

scholarly journals Asporin Restricts Mesenchymal Stromal Cell Differentiation, Alters the Tumor Microenvironment, and Drives Metastatic Progression

2019 ◽  
Vol 79 (14) ◽  
pp. 3636-3650 ◽  
Author(s):  
Robert M. Hughes ◽  
Brian W. Simons ◽  
Hamda Khan ◽  
Rebecca Miller ◽  
Valentina Kugler ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Tumor Microenvironment ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Metastatic Progression

scholarly journals Enriched osteogenic potential of CD317-negative mesenchymal stromal cell populations in vitro and in vivo

Bone Reports ◽  
2020 ◽  
Vol 13 ◽  
pp. 100509
Author(s):  
Alasdair G. Kay ◽  
James Fox ◽  
Andrew Stone ◽  
Sally James ◽  
Elizabeth Kapasa ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Osteogenic Potential ◽  
Cell Populations

Monitoring human mesenchymal stromal cell differentiation by electrochemical impedance sensing

Cytotherapy ◽  
2011 ◽  
Vol 13 (9) ◽  
pp. 1074-1089 ◽  
Author(s):  
Michael Angstmann ◽  
Irena Brinkmann ◽  
Karen Bieback ◽  
Dirk Breitkreutz ◽  
Christian Maercker
Keyword(s):  
Cell Differentiation ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Electrochemical Impedance ◽  
Impedance Sensing

scholarly journals Oleanolic Acid Enhances Mesenchymal Stromal Cell Osteogenic Potential by Inhibition of Notch Signaling

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Bing Shu ◽  
Yongjian Zhao ◽  
Yongjun Wang ◽  
Guangxi Wang ◽  
Xifu Shang ◽  
...  
Keyword(s):  
Notch Signaling ◽  
Oleanolic Acid ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Osteogenic Potential

scholarly journals Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2972
Author(s):  
Suzanne J. K. Mund ◽  
Daniel J. MacPhee ◽  
John Campbell ◽  
Ali Honaramooz ◽  
Bruce Wobeser ◽  
...  
Keyword(s):  
Cord Blood ◽  
Mrna Expression ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Wound Closure ◽  
Adverse Reactions ◽  
Transforming Growth Factor ◽  
Multipotent Mesenchymal Stromal Cell ◽  
Clinical Safety ◽  
Wound Size

Limb wounds are common in horses and often develop complications. Intravenous multipotent mesenchymal stromal cell (MSC) therapy is promising but has risks associated with intravenous administration and unknown potential to improve cutaneous wound healing. The objectives were to determine the clinical safety of administering large numbers of allogeneic cord blood-derived MSCs intravenously, and if therapy causes clinically adverse reactions, accelerates wound closure, improves histologic healing, and alters mRNA expression of common wound cytokines. Wounds were created on the metacarpus of 12 horses. Treatment horses were administered 1.51–2.46 × 108 cells suspended in 50% HypoThermosol FRS, and control horses were administered 50% HypoThermosol FRS alone. Epithelialization, contraction, and wound closure rates were determined using planimetric analysis. Wounds were biopsied and evaluated for histologic healing characteristics and cytokine mRNA expression. Days until wound closure was also determined. The results indicate that 3/6 of treatment horses and 1/6 of control horses experienced minor transient reactions. Treatment did not accelerate wound closure or improve histologic healing. Treatment decreased wound size and decreased all measured cytokines except transforming growth factor-β3. MSC intravenous therapy has the potential to decrease limb wound size; however, further work is needed to understand the clinical relevance of adverse reactions.

SERS Imaging of Mesenchymal Stromal Cell Differentiation

2021 ◽  
Author(s):  
Adrianna Milewska ◽  
Olafur E. Sigurjonsson ◽  
Kristjan Leosson
Keyword(s):  
Cell Differentiation ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Sers Imaging
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