High Sensitivity In-Cell EPR Distance Measurements on Proteins using an Optimized Gd(III) Spin Label

2018 ◽  
Vol 9 (20) ◽  
pp. 6119-6123 ◽  
Author(s):  
Yin Yang ◽  
Feng Yang ◽  
Yan-Jun Gong ◽  
Thorsten Bahrenberg ◽  
Akiva Feintuch ◽  
...  
Keyword(s):  
Spin Label ◽  
High Sensitivity ◽  
Distance Measurements

Semi-Rigid Nitroxide Spin Label for Long-Range EPR Distance Measurements of Lipid Bilayer Embedded β-Peptides

2019 ◽  
Vol 25 (9) ◽  
pp. 2203-2207 ◽  
Author(s):  
Janine Wegner ◽  
Gabriele Valora ◽  
Karin Halbmair ◽  
Annemarie Kehl ◽  
Brigitte Worbs ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Long Range ◽  
Spin Label ◽  
Distance Measurements

scholarly journals SLIM: A Short‐Linked, Highly Redox‐Stable Trityl Label for High‐Sensitivity In‐Cell EPR Distance Measurements

2020 ◽  
Vol 132 (24) ◽  
pp. 9854-9859 ◽  
Author(s):  
Nico Fleck ◽  
Caspar A. Heubach ◽  
Tobias Hett ◽  
Florian R. Haege ◽  
Pawel P. Bawol ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Distance Measurements

Generic tags for Mn(ii) and Gd(iii) spin labels for distance measurements in proteins

2017 ◽  
Vol 19 (39) ◽  
pp. 26944-26956 ◽  
Author(s):  
Yin Yang ◽  
Yan-Jun Gong ◽  
Aleksei Litvinov ◽  
Hong-Kai Liu ◽  
Feng Yang ◽  
...  
Keyword(s):  
Spin Label ◽  
Metal Ion ◽  
Coordination Mode ◽  
Distance Distribution ◽  
Spin Labels ◽  
Distance Measurements

The coordination mode of the metal ion in the spin label affects the distance distribution determined by DEER distance measurements.

scholarly journals Pulsed ELDOR Measurement of the Distance Between a Spin-Label and Copper (II) Centre in the Copper Loaded R48C Mutant of N. gonorrhoeae Ferric Binding Protein

2018 ◽  
Author(s):  
Matt Bawn ◽  
Justin Bradley ◽  
Fraser MacMillan
Keyword(s):  
Spin Label ◽  
Binding Protein ◽  
Double Resonance ◽  
Distance Measurements ◽  
Paramagnetic Resonance ◽  
Distance Determination ◽  
Pulsed Epr ◽  
Ferric Binding Protein ◽  
Relaxation Experiments ◽  
Electron Paramagnetic

AbstractDistance determination in proteins and biomolecules using pulsed EPR (electron paramagnetic resonance) techniques is becoming an increasingly popular and accessible technique. PELDOR (pulsed electron-electron double resonance), is a technique designed for distance determination over a nanoscopic scale. Here, ferric binding protein (Fbp) is used to demonstrate the practicability of this technique to Cu (II) Metalloproteins. PELDOR is usually applied to bi-radicals or endogenous radicals, and distance determination using pulsed EPR of metal containing centres in biomolecules has been restricted to relaxation experiments. PELDOR distance measurements between a Cu (II) ion and a nitroxide have previously only been reported for model compounds [1, 2].Fbp as the name suggests usually, contains a Fe (III) ion centre. For the purposes of this investigation the Fe (III) ion was removed and replaced by a Cu (II) ion, after a nitroxide spin-label was added to the Fbp using of site directed spin-labelling (SDSL). PELDOR was then applied to measure the distance between the two centres.Simulation methods were then employed to fully investigate these data and allow a quantitative interpretation of the copper nitroxide PELDOR data. The observed PELDOR time traces were analysed using DEER analysis[3].

scholarly journals A Gadolinium Spin Label with Both a Narrow Central Transition and Short Tether for Use in Double Electron Electron Resonance Distance Measurements

2019 ◽  
Vol 58 (5) ◽  
pp. 3015-3025 ◽  
Author(s):  
Anokhi Shah ◽  
Amandine Roux ◽  
Matthieu Starck ◽  
Jackie A. Mosely ◽  
Michael Stevens ◽  
...  
Keyword(s):  
Spin Label ◽  
Distance Measurements ◽  
Electron Resonance ◽  
Central Transition ◽  
Double Electron ◽  
Double Electron Electron Resonance

scholarly journals A Spirocyclohexyl Nitroxide Amino Acid Spin Label for Pulsed EPR Spectroscopy Distance Measurements

2010 ◽  
Vol 16 (19) ◽  
pp. 5778-5782 ◽  
Author(s):  
Andrzej Rajca ◽  
Velavan Kathirvelu ◽  
Sandip K. Roy ◽  
Maren Pink ◽  
Suchada Rajca ◽  
...  
Keyword(s):  
Amino Acid ◽  
Epr Spectroscopy ◽  
Spin Label ◽  
Distance Measurements ◽  
Pulsed Epr ◽  
Nitroxide Amino Acid

DEER distance measurements on trityl/trityl and Gd(iii)/trityl labelled proteins

2019 ◽  
Vol 21 (20) ◽  
pp. 10217-10227 ◽  
Author(s):  
Angeliki Giannoulis ◽  
Yin Yang ◽  
Yan-Jun Gong ◽  
Xiaoli Tan ◽  
Akiva Feintuch ◽  
...  
Keyword(s):  
Spin Label ◽  
Protein Conjugation ◽  
Distance Measurements

Trityl–trityl and trityl–Gd(iii) DEER distance measurements in proteins are performed using a new trityl spin label affording thioether–protein conjugation.

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