Semi-Rigid Nitroxide Spin Label for Long-Range EPR Distance Measurements of Lipid Bilayer Embedded β-Peptides

2019 ◽  
Vol 25 (9) ◽  
pp. 2203-2207 ◽  
Author(s):  
Janine Wegner ◽  
Gabriele Valora ◽  
Karin Halbmair ◽  
Annemarie Kehl ◽  
Brigitte Worbs ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Long Range ◽  
Spin Label ◽  
Distance Measurements

Spin Label Study of Apomembranes and Purple Membranes

1993 ◽  
Vol 48 (5-6) ◽  
pp. 500-503
Author(s):  
Tzvetana R. Lazarova ◽  
Maya Y. Velitchkova
Keyword(s):  
Fatty Acids ◽  
Lipid Bilayer ◽  
Spin Label ◽  
Hyperfine Splitting ◽  
Esr Spectra ◽  
Spin Labels ◽  
Label Study ◽  
Splitting Parameter ◽  
Induced Changes ◽  
Purple Membranes

Abstract Three spin-labelled fatty acids were used to detect the dynamics of lipid bilayer of apomem branes and purple membranes. It was found that ESR spectra of spin labels bound to apo­ membranes showed a temperature-induced changes rather similar to those seen with purple membranes. At the same time, however, the values of hyperfine splitting parameter 2Tm were lower as compared to purple membranes. The results pointed out that the removal of the retinal from purple membranes affects the dynamics of lipid bilayer and apomembranes were more rigid structure than those of purple membranes.

scholarly journals A triarylmethyl spin label for long-range distance measurement at physiological temperatures using T 1 relaxation enhancement

2016 ◽  
Vol 269 ◽  
pp. 50-54 ◽  
Author(s):  
Zhongyu Yang ◽  
Michael D. Bridges ◽  
Carlos J. López ◽  
Olga Yu. Rogozhnikova ◽  
Dmitry V. Trukhin ◽  
...  
Keyword(s):  
Long Range ◽  
Spin Label ◽  
Distance Measurement

High Sensitivity In-Cell EPR Distance Measurements on Proteins using an Optimized Gd(III) Spin Label

2018 ◽  
Vol 9 (20) ◽  
pp. 6119-6123 ◽  
Author(s):  
Yin Yang ◽  
Feng Yang ◽  
Yan-Jun Gong ◽  
Thorsten Bahrenberg ◽  
Akiva Feintuch ◽  
...  
Keyword(s):  
Spin Label ◽  
High Sensitivity ◽  
Distance Measurements

Efficient localization of a native metal ion within a protein by Cu2+-based EPR distance measurements

2019 ◽  
Vol 21 (20) ◽  
pp. 10238-10243 ◽  
Author(s):  
Austin Gamble Jarvi ◽  
Timothy F. Cunningham ◽  
Sunil Saxena
Keyword(s):  
Binding Site ◽  
Long Range ◽  
Metal Binding ◽  
Metal Ion ◽  
Native Metal ◽  
Distance Measurements ◽  
Metal Binding Site ◽  
Paramagnetic Metal

A native paramagnetic metal binding site in a protein is located with less than 2 Å resolution by a combination of double histidine (dHis) based Cu2+ labeling and long range distance measurements by EPR.

scholarly journals High-resolution measurement of long-range distances in RNA: pulse EPR spectroscopy with TEMPO-labeled nucleotides

2016 ◽  
Vol 7 (5) ◽  
pp. 3172-3180 ◽  
Author(s):  
Karin Halbmair ◽  
Jan Seikowski ◽  
Igor Tkach ◽  
Claudia Höbartner ◽  
Deniz Sezer ◽  
...  
Keyword(s):  
High Resolution ◽  
Long Range ◽  
Epr Spectroscopy ◽  
Base Pair ◽  
Distance Measurements ◽  
Model Free ◽  
High Resolution Measurement ◽  
Resolution Measurement ◽  
Pair Separation ◽  
Pulse Epr

Distance measurements in RNAs by pulse EPR with TEMPO-labeled nucleotides allow for model free conversion of distances into base-pair separation.

Generic tags for Mn(ii) and Gd(iii) spin labels for distance measurements in proteins

2017 ◽  
Vol 19 (39) ◽  
pp. 26944-26956 ◽  
Author(s):  
Yin Yang ◽  
Yan-Jun Gong ◽  
Aleksei Litvinov ◽  
Hong-Kai Liu ◽  
Feng Yang ◽  
...  
Keyword(s):  
Spin Label ◽  
Metal Ion ◽  
Coordination Mode ◽  
Distance Distribution ◽  
Spin Labels ◽  
Distance Measurements

The coordination mode of the metal ion in the spin label affects the distance distribution determined by DEER distance measurements.

Long-Range Distance Measurements on Nucleic Acids in Cells by Pulsed EPR Spectroscopy

2011 ◽  
Vol 50 (22) ◽  
pp. 5070-5074 ◽  
Author(s):  
Ivan Krstić ◽  
Robert Hänsel ◽  
Olga Romainczyk ◽  
Joachim W. Engels ◽  
Volker Dötsch ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Long Range ◽  
Epr Spectroscopy ◽  
Distance Measurements ◽  
Pulsed Epr ◽  
In Cells
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