scholarly journals A Spirocyclohexyl Nitroxide Amino Acid Spin Label for Pulsed EPR Spectroscopy Distance Measurements

2010 ◽  
Vol 16 (19) ◽  
pp. 5778-5782 ◽  
Author(s):  
Andrzej Rajca ◽  
Velavan Kathirvelu ◽  
Sandip K. Roy ◽  
Maren Pink ◽  
Suchada Rajca ◽  
...  
Keyword(s):  
Amino Acid ◽  
Epr Spectroscopy ◽  
Spin Label ◽  
Distance Measurements ◽  
Pulsed Epr ◽  
Nitroxide Amino Acid

Long-Range Distance Measurements on Nucleic Acids in Cells by Pulsed EPR Spectroscopy

2011 ◽  
Vol 50 (22) ◽  
pp. 5070-5074 ◽  
Author(s):  
Ivan Krstić ◽  
Robert Hänsel ◽  
Olga Romainczyk ◽  
Joachim W. Engels ◽  
Volker Dötsch ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Long Range ◽  
Epr Spectroscopy ◽  
Distance Measurements ◽  
Pulsed Epr ◽  
In Cells

scholarly journals Pulsed ELDOR Measurement of the Distance Between a Spin-Label and Copper (II) Centre in the Copper Loaded R48C Mutant of N. gonorrhoeae Ferric Binding Protein

2018 ◽  
Author(s):  
Matt Bawn ◽  
Justin Bradley ◽  
Fraser MacMillan
Keyword(s):  
Spin Label ◽  
Binding Protein ◽  
Double Resonance ◽  
Distance Measurements ◽  
Paramagnetic Resonance ◽  
Distance Determination ◽  
Pulsed Epr ◽  
Ferric Binding Protein ◽  
Relaxation Experiments ◽  
Electron Paramagnetic

AbstractDistance determination in proteins and biomolecules using pulsed EPR (electron paramagnetic resonance) techniques is becoming an increasingly popular and accessible technique. PELDOR (pulsed electron-electron double resonance), is a technique designed for distance determination over a nanoscopic scale. Here, ferric binding protein (Fbp) is used to demonstrate the practicability of this technique to Cu (II) Metalloproteins. PELDOR is usually applied to bi-radicals or endogenous radicals, and distance determination using pulsed EPR of metal containing centres in biomolecules has been restricted to relaxation experiments. PELDOR distance measurements between a Cu (II) ion and a nitroxide have previously only been reported for model compounds [1, 2].Fbp as the name suggests usually, contains a Fe (III) ion centre. For the purposes of this investigation the Fe (III) ion was removed and replaced by a Cu (II) ion, after a nitroxide spin-label was added to the Fbp using of site directed spin-labelling (SDSL). PELDOR was then applied to measure the distance between the two centres.Simulation methods were then employed to fully investigate these data and allow a quantitative interpretation of the copper nitroxide PELDOR data. The observed PELDOR time traces were analysed using DEER analysis[3].

Amplitude of Pancreatic Lipase Lid Opening in Solution and Identification of Spin Label Conformational Subensembles by Combining Continuous Wave and Pulsed EPR Spectroscopy and Molecular Dynamics

Biochemistry ◽  
2010 ◽  
Vol 49 (10) ◽  
pp. 2140-2149 ◽  
Author(s):  
Sebastien Ranaldi ◽  
Valérie Belle ◽  
Mireille Woudstra ◽  
Raphael Bourgeas ◽  
Bruno Guigliarelli ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Pancreatic Lipase ◽  
Epr Spectroscopy ◽  
Spin Label ◽  
Continuous Wave ◽  
Pulsed Epr

scholarly journals Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

2015 ◽  
Vol 51 (72) ◽  
pp. 13850-13853 ◽  
Author(s):  
Filip Wojciechowski ◽  
Andreas Groß ◽  
Isabelle T. Holder ◽  
Laura Knörr ◽  
Malte Drescher ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Epr Spectroscopy ◽  
Signal To Noise ◽  
Distance Measurements ◽  
Pulsed Epr

Gd3+ is increasingly used in intracellular EPR spectroscopy due to its increased superior stability and signal-to-noise ratios. We present a strategy for internally labeling nucleic acids with Gd3+.

A semi-rigid isoindoline-derived nitroxide spin label for RNA

2018 ◽  
Vol 16 (5) ◽  
pp. 816-824 ◽  
Author(s):  
Dnyaneshwar B. Gophane ◽  
Burkhard Endeward ◽  
Thomas F. Prisner ◽  
Snorri Th. Sigurdsson
Keyword(s):  
Epr Spectroscopy ◽  
Spin Label ◽  
Spin Labels ◽  
Pulsed Epr ◽  
Rna Duplexes ◽  
Limited Motion

The new semi-rigid spin label ImUm showed limited motion in RNA duplexes and accurate distances between two spin labels in RNA duplexes were obtained by pulsed EPR spectroscopy.

Long-Range Distance Measurements on Nucleic Acids in Cells by Pulsed EPR Spectroscopy

2011 ◽  
Vol 123 (22) ◽  
pp. 5176-5180 ◽  
Author(s):  
Ivan Krstić ◽  
Robert Hänsel ◽  
Olga Romainczyk ◽  
Joachim W. Engels ◽  
Volker Dötsch ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Long Range ◽  
Epr Spectroscopy ◽  
Distance Measurements ◽  
Pulsed Epr ◽  
In Cells

Semi-Rigid Nitroxide Spin Label for Long-Range EPR Distance Measurements of Lipid Bilayer Embedded β-Peptides

2019 ◽  
Vol 25 (9) ◽  
pp. 2203-2207 ◽  
Author(s):  
Janine Wegner ◽  
Gabriele Valora ◽  
Karin Halbmair ◽  
Annemarie Kehl ◽  
Brigitte Worbs ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Long Range ◽  
Spin Label ◽  
Distance Measurements

scholarly journals Aminoxyl Radicals of B/P Frustrated Lewis Pairs: Refinement of the Spin-Hamiltonian Parameters by Field- and Temperature-Dependent Pulsed EPR Spectroscopy

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157944 ◽  
Author(s):  
Marcos de Oliveira ◽  
Robert Knitsch ◽  
Muhammad Sajid ◽  
Annika Stute ◽  
Lisa-Maria Elmer ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Spin Hamiltonian ◽  
Frustrated Lewis Pairs ◽  
Temperature Dependent ◽  
Pulsed Epr ◽  
Hamiltonian Parameters ◽  
Aminoxyl Radicals ◽  
Lewis Pairs
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