Ion-Specific Protein/Water Interface Determines the Hofmeister Effect on the Kinetic Stability of Glucose Oxidase

2019 ◽  
Vol 123 (38) ◽  
pp. 7965-7973 ◽  
Author(s):  
Erik Sedlák ◽  
Dagmar Sedláková ◽  
Jozef Marek ◽  
Jozef Hančár ◽  
Katarína Garajová ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Specific Protein ◽  
Kinetic Stability ◽  
Water Interface ◽  
Hofmeister Effect

Study of glucose oxidase–l-α-lecithin Langmuir film formation on air–water interface

2007 ◽  
Vol 7 (5) ◽  
pp. 490-495 ◽  
Author(s):  
Fan Yin ◽  
Kil-Yong Ryu ◽  
Hoon-Kyu Shin ◽  
Young-Soo Kwon
Keyword(s):  
Glucose Oxidase ◽  
Film Formation ◽  
Water Interface ◽  
Langmuir Film ◽  
Air Water Interface

Adsorption–Penetration Studies of Glucose Oxidase into Phospholipid Monolayers at the 1,2-Dichloroethane/Water Interface

ChemPhysChem ◽  
2007 ◽  
Vol 8 (10) ◽  
pp. 1540-1547 ◽  
Author(s):  
Hélder A. Santos ◽  
Elisabete S. Ferreira ◽  
Elisa J. Pereira ◽  
Carlos M. Pereira ◽  
Kyösti Kontturi ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Water Interface ◽  
Phospholipid Monolayers

Glucose Sensor Based on Glucose Oxidase-Lipid LB Film Immobilized in Prussian Blue Layer

2008 ◽  
Vol 8 (9) ◽  
pp. 4543-4547 ◽  
Author(s):  
Dong-Yun Lee ◽  
A. K. M. Kafi ◽  
Won-Suk Choi ◽  
Sang-Hyun Park ◽  
Young-Soo Kwon
Keyword(s):  
Detection Limit ◽  
Prussian Blue ◽  
Glucose Oxidase ◽  
Modified Electrode ◽  
Glucose Sensor ◽  
Water Interface ◽  
Linear Calibration ◽  
Langmuir Film ◽  
Lipid Film ◽  
Air Water Interface

The structure and dynamic organization of a mixed Langmuir film of glucose oxidase (GOx) and lipid at the air–water interface were studied. The film was transferred onto the Prussian Blue (PB)-modified Pt electrode for biosensor preparation. The PB modified electrode showed well defined redox peaks in 0.1 M PBS electrolyte. The Langmuir film was characterized at the air–water interface by π-A isotherms. The mixed monolayer was formed by spreading GOx on the LB trough covered with lipid. Time-pressure results show that at least 90 minutes are required to reach the equilibrium state of GOx-lipid film. The monolayer was transferred onto the PB-modified electrode when surface pressure was 40 mN/m. This sensor was characterized by a very low detection limit and a wide linear range. The optimal conditions for both fabricating and response of the sensor were investigated. The proposed biosensor showed a linear calibration range from 5 × 10−6 to 6 × 10−5 M. The detection limit was determined to be 1.5 × 10−6 M.

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