Observation of the Adsorption of Cytochromecand Glucose Oxidase onto an Amphiphilic Monolayer at an Air/Water Interface by the Reflection Spectroscopy of Visible Radiation

1995 ◽  
Vol 68 (1) ◽  
pp. 230-236 ◽  
Author(s):  
Michihiro Ohnishi ◽  
Coe Ishimoto ◽  
Jun’etsu Seto
2007 ◽  
Vol 7 (5) ◽  
pp. 490-495 ◽  
Author(s):  
Fan Yin ◽  
Kil-Yong Ryu ◽  
Hoon-Kyu Shin ◽  
Young-Soo Kwon

Langmuir ◽  
2000 ◽  
Vol 16 (8) ◽  
pp. 3920-3926 ◽  
Author(s):  
Yoshie Urai ◽  
Chikaomi Ohe ◽  
Koichi Itoh ◽  
Masaaki Yoshida ◽  
Ken-ichi Iimura ◽  
...  

2008 ◽  
Vol 8 (9) ◽  
pp. 4543-4547 ◽  
Author(s):  
Dong-Yun Lee ◽  
A. K. M. Kafi ◽  
Won-Suk Choi ◽  
Sang-Hyun Park ◽  
Young-Soo Kwon

The structure and dynamic organization of a mixed Langmuir film of glucose oxidase (GOx) and lipid at the air–water interface were studied. The film was transferred onto the Prussian Blue (PB)-modified Pt electrode for biosensor preparation. The PB modified electrode showed well defined redox peaks in 0.1 M PBS electrolyte. The Langmuir film was characterized at the air–water interface by π-A isotherms. The mixed monolayer was formed by spreading GOx on the LB trough covered with lipid. Time-pressure results show that at least 90 minutes are required to reach the equilibrium state of GOx-lipid film. The monolayer was transferred onto the PB-modified electrode when surface pressure was 40 mN/m. This sensor was characterized by a very low detection limit and a wide linear range. The optimal conditions for both fabricating and response of the sensor were investigated. The proposed biosensor showed a linear calibration range from 5 × 10−6 to 6 × 10−5 M. The detection limit was determined to be 1.5 × 10−6 M.


RSC Advances ◽  
2017 ◽  
Vol 7 (10) ◽  
pp. 5872-5879 ◽  
Author(s):  
A. Mora-Boza ◽  
T. Lopes-Costa ◽  
F. Gámez ◽  
J. M. Pedrosa

In this work, ultraviolet-visible reflection spectroscopy is proposed as a technique that, in combination with classical surface pressure–area isotherms, allows to study in situ the adsorption of DNA to octadecylamine monolayers.


1999 ◽  
Vol 28 (5) ◽  
pp. 375-376 ◽  
Author(s):  
Takeshi Kawai ◽  
Ryoichi Hane ◽  
Fumitoshi Ishizaka ◽  
Kijiro Kon-No

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