Study of glucose oxidase–l-α-lecithin Langmuir film formation on air–water interface

2007 ◽  
Vol 7 (5) ◽  
pp. 490-495 ◽  
Author(s):  
Fan Yin ◽  
Kil-Yong Ryu ◽  
Hoon-Kyu Shin ◽  
Young-Soo Kwon
2008 ◽  
Vol 8 (9) ◽  
pp. 4543-4547 ◽  
Author(s):  
Dong-Yun Lee ◽  
A. K. M. Kafi ◽  
Won-Suk Choi ◽  
Sang-Hyun Park ◽  
Young-Soo Kwon

The structure and dynamic organization of a mixed Langmuir film of glucose oxidase (GOx) and lipid at the air–water interface were studied. The film was transferred onto the Prussian Blue (PB)-modified Pt electrode for biosensor preparation. The PB modified electrode showed well defined redox peaks in 0.1 M PBS electrolyte. The Langmuir film was characterized at the air–water interface by π-A isotherms. The mixed monolayer was formed by spreading GOx on the LB trough covered with lipid. Time-pressure results show that at least 90 minutes are required to reach the equilibrium state of GOx-lipid film. The monolayer was transferred onto the PB-modified electrode when surface pressure was 40 mN/m. This sensor was characterized by a very low detection limit and a wide linear range. The optimal conditions for both fabricating and response of the sensor were investigated. The proposed biosensor showed a linear calibration range from 5 × 10−6 to 6 × 10−5 M. The detection limit was determined to be 1.5 × 10−6 M.


2009 ◽  
Vol 139 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Ravi Kanth Kamlekar ◽  
M. Sharath Chandra ◽  
T.P. Radhakrishnan ◽  
Musti J. Swamy

2019 ◽  
Vol 124 (2) ◽  
pp. 1544-1553
Author(s):  
Elena S. Kartashynska ◽  
Yuri B. Vysotsky ◽  
Dieter Vollhardt ◽  
Valentin B. Fainerman ◽  
Anatoly Yu. Zakharov

2004 ◽  
Vol 13 (03n04) ◽  
pp. 347-353 ◽  
Author(s):  
M. SHARATH CHANDRA ◽  
Y. OGATA ◽  
J. KAWAMATA ◽  
T. P. RADHAKRISHNAN

Complexation of ionic amphiphiles at the air–water interface by polyelectrolytes in the subphase has a pronounced effect of stabilization and deaggregation on the amphiphiles. We have effectively utilized the latter in the case of a hemicyanine dye based amphiphile. The aggregation of these amphiphiles is influenced by the rate of equilibration of the Langmuir film and the introduction of polyelectrolytes in the subphase is found to be a convenient method to suppress the aggregation. Langmuir–Blodgett films obtained by transfer of the polyelectrolyte complexed amphiphiles show enhanced second harmonic generation.


1991 ◽  
Vol 71 (1-2) ◽  
pp. 147-152 ◽  
Author(s):  
M. Thomas ◽  
I.W. Kellaway ◽  
B.E. Jones

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