Structure-Based Design of Small Peptide Ligands to Inhibit Early-Stage Protein Aggregation Nucleation

2020 ◽  
Vol 60 (6) ◽  
pp. 3304-3314 ◽  
Author(s):  
Avinash Mishra ◽  
Rohit Bansal ◽  
Shravan Sreenivasan ◽  
Rozaleen Dash ◽  
Srishti Joshi ◽  
...  
Keyword(s):  
Protein Aggregation ◽  
Early Stage ◽  
Peptide Ligands ◽  
Small Peptide

Small Peptide Ligands for Affinity Separations of Biological Molecules

2005 ◽  
pp. 63-83 ◽  
Author(s):  
Guangquan Wang ◽  
Jeffrey R. Salm ◽  
Patrick V. Gurgel ◽  
Ruben G. Carbonell
Keyword(s):  
Peptide Ligands ◽  
Biological Molecules ◽  
Small Peptide

Development of novel small peptide ligands for antibody purification

RSC Advances ◽  
2015 ◽  
Vol 5 (82) ◽  
pp. 67093-67101 ◽  
Author(s):  
Yuping Wei ◽  
Jiandong Xu ◽  
Liang Zhang ◽  
Yankai Fu ◽  
Xia Xu
Keyword(s):  
Immunoglobulin G ◽  
Molecular Simulations ◽  
Peptide Ligands ◽  
Human Immunoglobulin ◽  
Small Peptide ◽  
Antibody Purification ◽  
Human Immunoglobulin G ◽  
Elution Condition ◽  
Potential Alternative

Small peptide ligands which were designed based on the interactions with human immunoglobulin G (IgG) using the molecular simulations, can offer a potential alternative for mAb purification with elution condition at pH 9 and pH 3.

scholarly journals Bacterial production and direct functional screening of expanded molecular libraries for discovering inhibitors of protein aggregation

2019 ◽  
Vol 5 (10) ◽  
pp. eaax5108 ◽  
Author(s):  
Dafni C. Delivoria ◽  
Sean Chia ◽  
Johnny Habchi ◽  
Michele Perni ◽  
Ilias Matis ◽  
...  
Keyword(s):  
Protein Aggregation ◽  
Early Stage ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Functional Screening ◽  
Parkinson’S Diseases ◽  
Aggregation Inhibitors ◽  
Molecular Libraries

Protein misfolding and aggregation are associated with a many human disorders, including Alzheimer’s and Parkinson’s diseases. Toward increasing the effectiveness of early-stage drug discovery for these conditions, we report a bacterial platform that enables the biosynthesis of molecular libraries with expanded diversities and their direct functional screening for discovering protein aggregation inhibitors. We illustrate this approach by performing, what is to our knowledge, the largest functional screen of small-size molecular entities described to date. We generated a combinatorial library of ~200 million drug-like, cyclic peptides and rapidly screened it for aggregation inhibitors against the amyloid-β peptide (Aβ42), linked to Alzheimer’s disease. Through this procedure, we identified more than 400 macrocyclic compounds that efficiently reduce Aβ42 aggregation and toxicity in vitro and in vivo. Finally, we applied a combination of deep sequencing and mutagenesis analyses to demonstrate how this system can rapidly determine structure-activity relationships and define consensus motifs required for bioactivity.

Monitoring Early-Stage Protein Aggregation by an Aggregation-Induced Emission Fluorogen

2017 ◽  
Vol 89 (17) ◽  
pp. 9322-9329 ◽  
Author(s):  
Manjeet Kumar ◽  
Yuning Hong ◽  
David C. Thorn ◽  
Heath Ecroyd ◽  
John A. Carver
Keyword(s):  
Protein Aggregation ◽  
Early Stage ◽  
Aggregation Induced Emission

The production of hydrogen peroxide during early-stage protein aggregation: a common pathological mechanism in different neurodegenerative diseases?

2005 ◽  
Vol 33 (4) ◽  
pp. 548-550 ◽  
Author(s):  
B.J. Tabner ◽  
S. Turnbull ◽  
N.J. Fullwood ◽  
M. German ◽  
D. Allsop
Keyword(s):  
Neurodegenerative Diseases ◽  
Protein Aggregation ◽  
Early Stage ◽  
Amyloid Β ◽  
Direct Production ◽  
Esr Spin Trapping ◽  
Pathological Mechanism ◽  
Aβ Amyloid ◽  
Production Of Hydrogen ◽  
Trapping Method

By means of an ESR spin-trapping method, we have shown that Aβ (amyloid β), α-synuclein and various toxic forms of the prion protein all appear to generate H2O2in vitro. A fundamental molecular mechanism underlying the pathogenesis of cell death in several different neurodegenerative diseases could be the direct production of H2O2 during the early stages of protein aggregation.

scholarly journals α-Synuclein expression and Nrf2 deficiency cooperate to aggravate protein aggregation, neuronal death and inflammation in early-stage Parkinson's disease

2012 ◽  
Vol 21 (14) ◽  
pp. 3173-3192 ◽  
Author(s):  
Isabel Lastres-Becker ◽  
Ayse Ulusoy ◽  
Nadia G. Innamorato ◽  
Gurdal Sahin ◽  
Alberto Rábano ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Protein Aggregation ◽  
Neuronal Death ◽  
Early Stage
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