Highly Sensitive Detection of Bovine β-Lactoglobulin with Wide Linear Dynamic Range Based on Platinum Nanoparticles Probe

2018 ◽  
Vol 66 (44) ◽  
pp. 11830-11838 ◽  
Author(s):  
Shengfa He ◽  
Xin Li ◽  
Yong Wu ◽  
Shandong Wu ◽  
Zhihua Wu ◽  
...  
Keyword(s):  
Platinum Nanoparticles ◽  
Dynamic Range ◽  
Sensitive Detection ◽  
Linear Dynamic Range ◽  
Linear Dynamic ◽  
Highly Sensitive ◽  
Wide Linear Dynamic Range ◽  
Highly Sensitive Detection ◽  
Β Lactoglobulin

scholarly journals Development of a Quantitative Real-Time PCR Assay for Detection of Toxoplasma gondii in Brain Samples

2021 ◽  
Author(s):  
Mahboubeh Berizi ◽  
Jalal Babaie ◽  
Pezhman Fard-Esfahani ◽  
Marjan Enshaeieh ◽  
Rahmah Noordin ◽  
...  
Keyword(s):  
Detection Limit ◽  
Toxoplasma Gondii ◽  
Real Time Pcr ◽  
Genomic Dna ◽  
Dynamic Range ◽  
Quantitative Real Time Pcr ◽  
Linear Dynamic Range ◽  
Standard Curve ◽  
Linear Dynamic ◽  
Highly Sensitive

Background: Toxoplasmosis is a worldwide-distributed infection that can cause serious diseases, mainly in congenitally infected and immunodeficient individuals. PCR assays play an indispensable role in the detection of Toxoplasma gondii in different biological samples. Methods: This study was conducted in the Parasitology Department at Pasteur Institute of Iran (Tehran) during 2016-2018. We designed a highly sensitive quantitative real-time PCR (RT-qPCR) targeted REP-529, a noncoding repetitive DNA. We cloned the amplicon in a plasmid (pTZREP-529) and used it to generate the standard curve. The Toxoplasma RT-qPCR characteristics, i.e., detection limit, specificity, linear dynamic range, linearity, intra-, and inter-assay precisions, were determined. The detection limit of the assay was one plasmid copy number (PCN) per reaction (about 0.004 T. gondii genome), and the linear dynamic range was equal to 6 logs (1× 101 to 1× 107 PCN per reaction). Results: The assay showed no signal when genomic DNA of Plasmodium falciparum, Leishmania major, and Trichomonas vaginallis were used. The standard curve was drawn using dilutions of pTZREP-529 plasmid spiked with genomic DNA from a mouse brain, and test characteristics were shown unaffected. Applying the Toxoplasma RT-qPCR, we showed brain cysts were significantly decreased in mice vaccinated with GRA2 antigen of Toxoplasma formulated in Monophosphoryl Lipid A (MPL) adjuvant. Conclusion: We have developed a quantitative, specific, and highly sensitive PCR for detecting T. gondii in biological samples.

scholarly journals A Wide Linear Dynamic Range Image Sensor Based on Asynchronous Self-Reset and Tagging of Saturation Events

2017 ◽  
Vol 52 (6) ◽  
pp. 1605-1617 ◽  
Author(s):  
Juan Antonio Lenero-Bardallo ◽  
Ricardo Carmona-Galan ◽  
Angel Rodriguez-Vazquez
Keyword(s):  
Dynamic Range ◽  
Image Sensor ◽  
Range Image ◽  
Linear Dynamic Range ◽  
Linear Dynamic ◽  
Wide Linear Dynamic Range

scholarly journals First Report for Determination of d-Penicillamine in the Presence of Tryptophan Using a 2-Chlorobenzoyl Ferrocene/Ag-Supported ZnO Nanoplate–Modified Carbon Paste Electrode

2018 ◽  
Vol 101 (1) ◽  
pp. 208-215 ◽  
Author(s):  
Hadi Beitollahi ◽  
Abbas Gholami ◽  
Mohammad Reza Ganjali
Keyword(s):  
Carbon Paste Electrode ◽  
High Selectivity ◽  
Dynamic Range ◽  
Modified Carbon Paste Electrode ◽  
Linear Dynamic Range ◽  
Carbon Paste ◽  
Linear Dynamic ◽  
Wide Linear Dynamic Range ◽  
Paste Electrode

Abstract In this work, a carbon paste electrode modified with Ag-ZnO nanoplates and 2-chlorobenzoyl ferrocene was prepared and applied for the determination of d-penicillamine in the presence of tryptophan. The morphologies of Ag-ZnO nanoplates were examined by scanning electron microscopy. It was found that under an optimum condition (pH 7.0), the oxidation of d-penicillamine at the surface of such an electrode occurs at a potential about 165 mV less positive than that of an unmodified carbon paste electrode. The diffusion coefficient (D = 7.6 × 10−6 cm2/s) and the electron transfer coefficient (α = 0.54) for d-penicillamine oxidation were also determined. The proposed method exhibited a wide linear dynamic range of 0.03–250.0 µM, with an LOD (S/N = 3) of 0.015 µM. Moreover, the modified electrode exhibited good reproducibility and high selectivity, demonstrating its feasibility for the analytical purpose. Lastly, this new sensor was used for the determination of d-penicillamine and tryptophan in real samples.

Ultrathin Single‐Crystalline 2D Perovskite Photoconductor for High‐Performance Narrowband and Wide Linear Dynamic Range Photodetection

Small ◽  
2020 ◽  
Vol 16 (52) ◽  
pp. 2005626
Author(s):  
Yudi Tu ◽  
Yan Xu ◽  
Junzi Li ◽  
Qiaoyan Hao ◽  
Xiaosong Liu ◽  
...  
Keyword(s):  
High Performance ◽  
Dynamic Range ◽  
Linear Dynamic Range ◽  
Single Crystalline ◽  
Linear Dynamic ◽  
Wide Linear Dynamic Range

Highly Sensitive and Broadband Organic Photodetectors with Fast Speed Gain and Large Linear Dynamic Range at Low Forward Bias

Small ◽  
2017 ◽  
Vol 13 (24) ◽  
pp. 1603260 ◽  
Author(s):  
Riming Nie ◽  
Xianyu Deng ◽  
Lei Feng ◽  
Guiguang Hu ◽  
Yangyang Wang ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Forward Bias ◽  
Linear Dynamic Range ◽  
Linear Dynamic ◽  
Fast Speed ◽  
Highly Sensitive ◽  
Organic Photodetectors

Highly sensitive all-polymer photodetectors with ultraviolet-visible to near-infrared photo-detection and its application as optical switch

2021 ◽  
Author(s):  
Zijin Zhao ◽  
Baiqiao Liu ◽  
Chunyu Xu ◽  
Ming Liu ◽  
Kaixuan Yang ◽  
...  
Keyword(s):  
External Quantum Efficiency ◽  
Near Infrared ◽  
Dynamic Range ◽  
Optical Switch ◽  
Signal To Noise Ratio ◽  
Linear Dynamic Range ◽  
Signal To Noise ◽  
Linear Dynamic ◽  
Highly Sensitive ◽  
Photo Detection

All-polymer photodetectors with photomultiplication are fabricated with PMBBDT and N2200 as photoactive layers, exhibiting external quantum efficiency of 20700% under 4 V. Signal-to-noise ratio, linear dynamic range and specific detectivity...

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