Peptides from Extruded Lupin (Lupinus albus L.) Regulate Inflammatory Activity via the p38 MAPK Signal Transduction Pathway in RAW 264.7 Cells

2020 ◽  
Vol 68 (42) ◽  
pp. 11702-11709
Author(s):  
Yue Gao ◽  
Xuna Zhang ◽  
Guixing Ren ◽  
Caie Wu ◽  
Peiyou Qin ◽  
...  
Keyword(s):  
Signal Transduction ◽  
P38 Mapk ◽  
Lupinus Albus ◽  
Signal Transduction Pathway ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Transduction Pathway ◽  
Lupinus Albus L

scholarly journals Octominin Inhibits LPS-Induced Chemokine and Pro-inflammatory Cytokine Secretion from RAW 264.7 Macrophages via Blocking TLRs/NF-κB Signal Transduction

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 511 ◽  
Author(s):  
K. K. Asanka Sanjeewa ◽  
D. P. Nagahawatta ◽  
Hye-Won Yang ◽  
Jae Young Oh ◽  
Thilina U. Jayawardena ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Inflammatory Cytokine ◽  
Inflammatory Responses ◽  
Signal Transduction Pathway ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Potential Candidate ◽  
Transduction Pathway ◽  
Raw 264.7 Macrophages ◽  
In Silico Docking

Inflammation is a well-organized innate immune response that plays an important role during the pathogen attacks and mechanical injuries. The Toll-like receptors (TLR)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is a major signal transduction pathway observed in RAW 264.7 macrophages during the inflammatory responses. Here, we investigated the anti-inflammatory effects of Octominin; a bio-active peptide developed from Octopus minor in RAW 264.7 macrophages in vitro. Octominin was found to inhibit lipopolysaccharides (LPS)-stimulated transcriptional activation of NF-κB in RAW 264.7 cells and dose-dependently decreased the mRNA expression levels of TLR4. Specifically, in silico docking results demonstrated that Octominin has a potential to inhibit TLR4 mediated inflammatory responses via blocking formation of TLR4/MD-2/LPS complex. We also demonstrated that Octominin could significantly inhibit LPS-induced secretion of pro-inflammatory cytokine (interleukin-β; IL-1β, IL-6, and tumor necrosis factor-α) and chemokines (CCL3, CCL4, CCL5, and CXCL10) from RAW 264.7 cells. Additionally, Octominin repressed the LPS-induced pro-inflammatory mediators including nitric oxide (NO), prostaglandin E2, inducible NO synthase, and cyclooxygenase 2 in macrophages. These results suggest that Octominin is a potential inhibitor of TLRs/NF-κB signal transduction pathway and is a potential candidate for the treatment of inflammatory diseases.

scholarly journals A Novel Korean Red Ginseng Compound Gintonin Inhibited Inflammation by MAPK and NF-κB Pathways and Recovered the Levels of mir-34a and mir-93 in RAW 264.7 Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Evelyn Saba ◽  
Bo Ra Jeon ◽  
Da-Hye Jeong ◽  
Kija Lee ◽  
Youn-Kyoung Goo ◽  
...  
Keyword(s):  
Signal Transduction ◽  
The Body ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Therapeutic Effects ◽  
Raw 264.7 ◽  
Korean Red Ginseng ◽  
Health Promoting ◽  
Anti Inflammatory ◽  
First Time

The beneficial health promoting effects of ginseng from vitalizing the body to enhancing long life have been well explored very rapidly in the past few years. Up till now many ginsenosides have been discovered for their marvelous therapeutic effects. However during past three years, a novel ginseng compound has been discovered, called gintonin, that differs from other ginsenosides on the basis of its signal transduction and chemical nature. Gintonin has been widely studied for its anti-Alzheimer’s disease activities and other neuropathies. However, its anti-inflammatory activity remained unexplored. In our study we have reported for the first time the anti-inflammatory activity of gintonin on RAW 264.7 cells. We found that gintonin potently suppresses the nitric oxide production without any cytotoxicity at given doses and also efficiently suppressed the levels of proinflammatory cytokines. Moreover, it mediaes its signal transduction via MAPK and NF-κB pathways and revives the levels of mir-34a and mir-93. These findings are valuable for the anti-inflammatory effects of this new compound with particular reference to microRNA involvement in the ginseng family.

Regulation of RAW 264.7 cell-mediated immunity by polysaccharides from Agaricus blazei Murill via the MAPK signal transduction pathway

2017 ◽  
Vol 8 (4) ◽  
pp. 1475-1480 ◽  
Author(s):  
Feier Cheng ◽  
Xiaoyan Yan ◽  
Miaoqing Zhang ◽  
Mingchang Chang ◽  
Shaojun Yun ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Signal Transduction Pathway ◽  
Cell Mediated Immunity ◽  
Raw 264.7 ◽  
Transduction Pathway ◽  
Agaricus Blazei ◽  
Raw 264.7 Cell ◽  
Folk Remedy ◽  
Agaricus Blazei Murill

Agaricus blazei Murill (ABM) is a common anticancer folk remedy.

CK2 Is Acting Upstream of MEK3/6 as a Part of the Signal Control of ERK1/2 and p38 MAPK during Keratinocytes Autocrine Differentiation

2011 ◽  
Vol 66 (1-2) ◽  
pp. 83-86 ◽  
Author(s):  
Antonia R. Isaeva ◽  
Vanio I. Mitev
Keyword(s):  
Signal Transduction ◽  
P38 Mapk ◽  
Mammalian Cells ◽  
Protein Kinase Ck2 ◽  
Cell Signalling ◽  
Signal Transduction Pathway ◽  
Signal Control ◽  
Transduction Pathway ◽  
Protein Levels ◽  
Kinase Ck2

Protein kinase CK2 (formerly termed “casein kinase II”) is a ubiquitously in mammalian cells distributed Ser/Thr kinase, with global role in cell regulation. Although, the involvement of CK2 in cell signalling is vast-investigated, virtually nothing is known about its contribution to signal control of keratinocytes differentiation. Here we show that, in autocrine differentiating keratinocytes, inhibition of the CK2 activity induced by 4,5,6,7-tetrabromobenzotriazole (TBB) causes reciprocal changes in the activities of major signal transduction regulators of keratinocytes differentiation, i.e. ERK1/2 and p38 MAPK, without affecting their protein levels. The ERK1/2 activity is strongly suppressed, while the activity of p38 is increased. We have also found that the activity of upstream and specifi c for p38 MAPK kinase MEK3/6 is also stimulated by TBB. These original results clearly demonstrate the participation of CK2 in the signal transduction pathway controlling MEK3/6, p38 MAPK, and ERK1/2 in the used model system.

scholarly journals Involvement of the p38 MAPK-NF-κB Signal Transduction Pathway and COX-2 in the Pathobiology of Meniscus Degeneration in Humans

2008 ◽  
Vol 14 (3-4) ◽  
pp. 160-166 ◽  
Author(s):  
Dionysios J. Papachristou ◽  
Eugenia Papadakou ◽  
Efthimia K. Basdra ◽  
Panagiotis Baltopoulos ◽  
Elias Panagiotopoulos ◽  
...  
Keyword(s):  
Signal Transduction ◽  
P38 Mapk ◽  
Signal Transduction Pathway ◽  
Transduction Pathway ◽  
Meniscus Degeneration ◽  
Cox 2
Sign in / Sign up

Export Citation Format

Share Document