Regulation of RAW 264.7 cell-mediated immunity by polysaccharides from Agaricus blazei Murill via the MAPK signal transduction pathway

2017 ◽  
Vol 8 (4) ◽  
pp. 1475-1480 ◽  
Author(s):  
Feier Cheng ◽  
Xiaoyan Yan ◽  
Miaoqing Zhang ◽  
Mingchang Chang ◽  
Shaojun Yun ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Signal Transduction Pathway ◽  
Cell Mediated Immunity ◽  
Raw 264.7 ◽  
Transduction Pathway ◽  
Agaricus Blazei ◽  
Raw 264.7 Cell ◽  
Folk Remedy ◽  
Agaricus Blazei Murill

Agaricus blazei Murill (ABM) is a common anticancer folk remedy.

scholarly journals Octominin Inhibits LPS-Induced Chemokine and Pro-inflammatory Cytokine Secretion from RAW 264.7 Macrophages via Blocking TLRs/NF-κB Signal Transduction

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 511 ◽  
Author(s):  
K. K. Asanka Sanjeewa ◽  
D. P. Nagahawatta ◽  
Hye-Won Yang ◽  
Jae Young Oh ◽  
Thilina U. Jayawardena ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Inflammatory Cytokine ◽  
Inflammatory Responses ◽  
Signal Transduction Pathway ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Potential Candidate ◽  
Transduction Pathway ◽  
Raw 264.7 Macrophages ◽  
In Silico Docking

Inflammation is a well-organized innate immune response that plays an important role during the pathogen attacks and mechanical injuries. The Toll-like receptors (TLR)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is a major signal transduction pathway observed in RAW 264.7 macrophages during the inflammatory responses. Here, we investigated the anti-inflammatory effects of Octominin; a bio-active peptide developed from Octopus minor in RAW 264.7 macrophages in vitro. Octominin was found to inhibit lipopolysaccharides (LPS)-stimulated transcriptional activation of NF-κB in RAW 264.7 cells and dose-dependently decreased the mRNA expression levels of TLR4. Specifically, in silico docking results demonstrated that Octominin has a potential to inhibit TLR4 mediated inflammatory responses via blocking formation of TLR4/MD-2/LPS complex. We also demonstrated that Octominin could significantly inhibit LPS-induced secretion of pro-inflammatory cytokine (interleukin-β; IL-1β, IL-6, and tumor necrosis factor-α) and chemokines (CCL3, CCL4, CCL5, and CXCL10) from RAW 264.7 cells. Additionally, Octominin repressed the LPS-induced pro-inflammatory mediators including nitric oxide (NO), prostaglandin E2, inducible NO synthase, and cyclooxygenase 2 in macrophages. These results suggest that Octominin is a potential inhibitor of TLRs/NF-κB signal transduction pathway and is a potential candidate for the treatment of inflammatory diseases.

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