New Mechanism of Gemcitabine and Its Phosphates: DNA Polymerization Disruption via 3′–5′ Exonuclease Inhibition

Biochemistry ◽  
2020 ◽  
Vol 59 (45) ◽  
pp. 4344-4352
Author(s):  
Shuzhang Yang ◽  
Danyan Luo ◽  
Na Li ◽  
Chuncheng Li ◽  
Shuo Tang ◽  
...  
Keyword(s):  
Dna Polymerization

Stepping Statistics of Single HIV-1 Reverse Transcriptase Molecules during DNA Polymerization

2005 ◽  
Vol 109 (33) ◽  
pp. 16127-16131 ◽  
Author(s):  
Theodore P. Ortiz ◽  
Jason A. Marshall ◽  
Lauren A. Meyer ◽  
Ryan W. Davis ◽  
Jed C. Macosko ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Dna Polymerization ◽  
Hiv 1

Synthesis of Selenium‐Triphosphates (dNTPαSe) for More Specific DNA Polymerization

2019 ◽  
Vol 131 (23) ◽  
pp. 7917-7921
Author(s):  
Bei Hu ◽  
Yitao Wang ◽  
Shichao Sun ◽  
Weizhu Yan ◽  
Chong Zhang ◽  
...  
Keyword(s):  
Dna Polymerization

scholarly journals Distinct Requirement for Two Stages of Protein-Primed Initiation of Reverse Transcription in Hepadnaviruses

2002 ◽  
Vol 76 (12) ◽  
pp. 5857-5865 ◽  
Author(s):  
Xingtai Wang ◽  
Jianming Hu
Keyword(s):  
Reverse Transcription ◽  
Specific Interaction ◽  
Initial Step ◽  
Covalent Attachment ◽  
Dna Polymerization ◽  
Duck Hepatitis ◽  
B Virus ◽  
Protein Priming ◽  
Complete Protein ◽  
Two Stages

ABSTRACT Reverse transcription in hepadnaviruses is primed by the viral reverse transcriptase (RT) (protein priming) and requires the specific interaction between the RT and a viral RNA signal termed ε, which bears the specific template sequence for protein priming. The product of protein priming is a short oligodeoxynucleotide which represents the 5′ end of the viral minus-strand DNA and is covalently attached to the RT. We have now identified truncated RT variants from the duck hepatitis B virus that were fully active in the initial step of protein priming, i.e., the covalent attachment of the first nucleotide to the protein (RT deoxynucleotidylation), but defective in any subsequent DNA polymerization. A short sequence in the RT domain was localized that was dispensable for RT deoxynucleotidylation but essential for the subsequent DNA polymerization. These results have thus revealed two distinct stages of protein priming, i.e., the initial attachment of the first nucleotide to the RT (RT deoxynucleotidylation or initiation of protein priming) and the subsequent DNA synthesis (polymerization) to complete protein priming, with the second step entailing additional RT sequences. Two models are proposed to explain the observed differential sequence requirement for the two distinct stages of the protein priming reaction.

scholarly journals Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization

2009 ◽  
Vol 37 (8) ◽  
pp. e58-e58 ◽  
Author(s):  
Douglas D. Young ◽  
Hrvoje Lusic ◽  
Mark O. Lively ◽  
Alexander Deiters
Keyword(s):  
Restriction Enzyme ◽  
Light Regulation ◽  
Dna Polymerization

Detecting miRNA by producing RNA: a sensitive assay that combines rolling-circle DNA polymerization and rolling circle transcription

2015 ◽  
Vol 51 (60) ◽  
pp. 11976-11979 ◽  
Author(s):  
Xuemei Li ◽  
Fuwei Zheng ◽  
Rui Ren
Keyword(s):  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Sensitive Assay ◽  
Rolling Circle ◽  
Dna Polymerization ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Signal Production ◽  
Enhanced Raman Scattering ◽  
Dual Amplification

Target miRNA was detected by producing RNA: rolling circle polymerization (RCP) and rolling circle transcription (RCT) were interlinked to provide dual amplification, which was coupled with SERS (surface-enhanced Raman scattering) for signal production.

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