Elucidating the Kinetic Mechanism of DNA Polymerization Catalyzed bySulfolobus solfataricusP2 DNA Polymerase B1

Biochemistry ◽  
2009 ◽  
Vol 48 (31) ◽  
pp. 7502-7511 ◽  
Author(s):  
Jessica A. Brown ◽  
Zucai Suo
Keyword(s):  
Dna Polymerase ◽  
Kinetic Mechanism ◽  
Dna Polymerization

scholarly journals Kinetic Mechanism of DNA Polymerization Catalyzed by Human DNA Polymerase ε

Biochemistry ◽  
2013 ◽  
Vol 52 (40) ◽  
pp. 7041-7049 ◽  
Author(s):  
Walter J. Zahurancik ◽  
Seth J. Klein ◽  
Zucai Suo
Keyword(s):  
Dna Polymerase ◽  
Kinetic Mechanism ◽  
Dna Polymerization ◽  
Human Dna

scholarly journals Kinetic Mechanism of Active Site Assembly and Chemical Catalysis of DNA Polymerase β

Biochemistry ◽  
2011 ◽  
Vol 50 (45) ◽  
pp. 9865-9875 ◽  
Author(s):  
Paul B. Balbo ◽  
Eric Chun-Wei Wang ◽  
Ming-Daw Tsai
Keyword(s):  
Dna Polymerase ◽  
Active Site ◽  
Kinetic Mechanism ◽  
Dna Polymerase Β ◽  
Chemical Catalysis

The Kinetic Mechanism of Formation of the Bacteriophage T4 DNA Polymerase Sliding Clamp

1996 ◽  
Vol 264 (3) ◽  
pp. 440-452 ◽  
Author(s):  
Mark C. Young ◽  
Steven E. Weitzel ◽  
Peter H. von Hippel
Keyword(s):  
Dna Polymerase ◽  
Bacteriophage T4 ◽  
Kinetic Mechanism ◽  
Mechanism Of Formation ◽  
Sliding Clamp

scholarly journals Kinetic Mechanism of Translocation and dNTP Binding in Individual DNA Polymerase Complexes

2013 ◽  
Vol 135 (24) ◽  
pp. 9149-9155 ◽  
Author(s):  
Kate R. Lieberman ◽  
Joseph M. Dahl ◽  
Ai H. Mai ◽  
Ashley Cox ◽  
Mark Akeson ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Kinetic Mechanism ◽  
Dntp Binding

scholarly journals Cyclopropavir Susceptibility of Cytomegalovirus DNA Polymerase Mutants Selected after Antiviral Drug Exposure

2011 ◽  
Vol 56 (1) ◽  
pp. 197-201 ◽  
Author(s):  
Sunwen Chou ◽  
Gail Marousek ◽  
Terry L. Bowlin
Keyword(s):  
Dna Polymerase ◽  
Drug Exposure ◽  
Antiviral Drug ◽  
Cross Resistance ◽  
Yield Reduction ◽  
Low Grade ◽  
Dna Polymerization ◽  
Reduction Assay ◽  
Polymerase Mutants ◽  
Yield Reduction Assay

ABSTRACTHuman cytomegalovirus (CMV) UL54 DNA polymerase (pol) mutants with known patterns of resistance to current antivirals ganciclovir (GCV), foscarnet (FOS), and cidofovir (CDV) were tested for cyclopropavir (CPV) susceptibility by a standardized reporter-based yield reduction assay. Exonuclease and A987G (region V) mutations at codons commonly associated with dual GCV-CDV resistance in clinical isolates paradoxically conferred increased CPV susceptibility. Various polymerase catalytic region mutations conferring FOS resistance with variable low-grade GCV and CDV cross-resistance also conferred CPV resistance, with 50% effective concentration (EC50) increases of 3- to 13-fold. CPV EC50values against severalpolmutants were increased about 2-fold by adding UL97 mutation C592G. Propagation of a CMV exonuclease mutant under CPV selected forpolmutations less often than UL97 mutations. In 21 experiments, one instance each of mutations E756D and M844V, which were shown individually to confer 3- to 4-fold increases in CPV EC50, was detected. Unlike GCV and CDV, exonuclease mutations are not a preferred mechanism of CPV resistance, but mutations in and nearpolregion III may confer CPV resistance by affecting its recognition as an incoming base for DNA polymerization.

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