Detecting miRNA by producing RNA: a sensitive assay that combines rolling-circle DNA polymerization and rolling circle transcription

2015 ◽  
Vol 51 (60) ◽  
pp. 11976-11979 ◽  
Author(s):  
Xuemei Li ◽  
Fuwei Zheng ◽  
Rui Ren
Keyword(s):  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Sensitive Assay ◽  
Rolling Circle ◽  
Dna Polymerization ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Signal Production ◽  
Enhanced Raman Scattering ◽  
Dual Amplification

Target miRNA was detected by producing RNA: rolling circle polymerization (RCP) and rolling circle transcription (RCT) were interlinked to provide dual amplification, which was coupled with SERS (surface-enhanced Raman scattering) for signal production.

scholarly journals Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Hairui Ren ◽  
Zhaoyang Chen ◽  
Xin Zhang ◽  
Yongmei Zhao ◽  
Zheng Wang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Linear Relationship ◽  
Silicon Nanowire ◽  
Nanowire Array ◽  
Surface Enhanced Raman Scattering ◽  
Sers Substrate ◽  
Rolling Circle ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Concentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC). The Ag nanoparticle/silicon nanowire array substrate was fabricated and employed as an active SERS substrate to indirectly measure the SAM concentration. The linear relationship between the integrated intensity of peak centered at ~2920 cm−1in SERS spectra and the SAM concentration was established, and the limit of detections of SAM concentrations was analyzed to be ~0.1 g/L. The concentration of SAM in real solution could be predicted by the linear relationship and verified by the HPLC detection method. The relative deviations (δ) of the predicted SAM concentration are less than 13% and the correlation coefficient is 0.9998. Rolling-Circle Filter was utilized to subtract fluorescence background and the optimal results were obtained when the radius of the analyzing circle is 650 cm−1.

A high sensitive assay platform based on surface-enhanced Raman scattering for quantification of protease activity

Talanta ◽  
2010 ◽  
Vol 82 (2) ◽  
pp. 631-639 ◽  
Author(s):  
Nazife Nur Yazgan ◽  
İsmail Hakkı Boyacı ◽  
Erhan Temur ◽  
Uğur Tamer ◽  
Ali Topcu
Keyword(s):  
Raman Scattering ◽  
Protease Activity ◽  
Surface Enhanced Raman Scattering ◽  
Sensitive Assay ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering
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