In Vivo Voltammetric Detection of Rat Brain Lactate with Carbon Fiber Microelectrodes Coated with Lactate Oxidase

1998 ◽  
Vol 70 (13) ◽  
pp. 2618-2622 ◽  
Author(s):  
Nataliya F. Shram ◽  
Larissa I. Netchiporouk ◽  
Claude Martelet ◽  
Nicole Jaffrezic-Renault ◽  
Chantal Bonnet ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Rat Brain ◽  
Lactate Oxidase ◽  
Voltammetric Detection ◽  
Carbon Fiber Microelectrodes ◽  
Brain Lactate

Carbon Nanotube-Modified Carbon Fiber Microelectrodes for In Vivo Voltammetric Measurement of Ascorbic Acid in Rat Brain

2007 ◽  
Vol 79 (17) ◽  
pp. 6559-6565 ◽  
Author(s):  
Meining Zhang ◽  
Kun Liu ◽  
Ling Xiang ◽  
Yuqing Lin ◽  
Lei Su ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Carbon Nanotube ◽  
Carbon Fiber ◽  
Rat Brain ◽  
Voltammetric Measurement ◽  
Carbon Fiber Microelectrodes

Application of fast-scan cyclic voltammetry for the in vivo characterization of optically evoked dopamine in the olfactory tubercle of the rat brain

The Analyst ◽  
2016 ◽  
Vol 141 (12) ◽  
pp. 3746-3755 ◽  
Author(s):  
Ken T. Wakabayashi ◽  
Michael J. Bruno ◽  
Caroline E. Bass ◽  
Jinwoo Park
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Rat Brain ◽  
Olfactory Tubercle ◽  
Fast Scan Cyclic Voltammetry ◽  
Carbon Fiber Microelectrodes ◽  
In Vivo Characterization

Dopamine regulation in the rat brain olfactory tubercle was characterized by fast-scan cyclic voltammetry coupled with carbon–fiber microelectrodes and optogenetics.

scholarly journals Modified Sawhorse Waveform for the Voltammetric Detection of Oxytocin

2022 ◽  
Author(s):  
Favian Liu ◽  
Negar Ghasem Ardabili ◽  
Izaiah Brown ◽  
Harmain Rafi ◽  
Clarice Cook ◽  
...  
Keyword(s):  
Brain Slices ◽  
Physiological Role ◽  
Peptide Hormone ◽  
Protective Effects ◽  
Fast Scan Cyclic Voltammetry ◽  
The Past ◽  
Voltammetric Detection ◽  
Carbon Fiber Microelectrodes

Abstract Carbon fiber microelectrodes (CFMEs) have been used to detect neurotransmitters and other biomolecules using fast-scan cyclic voltammetry (FSCV) for the past few decades. This technique measures neurotransmitters such as dopamine and, more recently, physiologically relevant neuropeptides. Oxytocin, a pleiotropic peptide hormone, is physiologically important for adaptation, development, reproduction, and social behavior. This neuropeptide functions as a stress-coping molecule, an anti-inflammatory agent, and serves as an antioxidant with protective effects especially during adversity or trauma. Here, we measure tyrosine using the Modified Sawhorse Waveform (MSW), enabling enhanced electrode sensitivity for the amino acid and oxytocin peptide. Applying the MSW, decreased surface fouling and enabled codetection with other monoamines. As oxytocin contains tyrosine, the MSW was also used to detect oxytocin. The sensitivity of oxytocin detection was found to be 3.99 ± 0.49 nA/µM, (n=5). Additionally, we demonstrate that applying the MSW on CFMEs allows for real time measurements of exogenously applied oxytocin on rat brain slices. These studies may serve as novel assays for oxytocin detection in a fast, sub-second timescale with possible implications for in vivo measurements and further understanding of the physiological role of oxytocin.

In Vivo Monitoring of Oxygen in Rat Brain by Carbon Fiber Microelectrode Modified with Antifouling Nanoporous Membrane

2019 ◽  
Vol 91 (5) ◽  
pp. 3645-3651 ◽  
Author(s):  
Lin Zhou ◽  
Hanfeng Hou ◽  
Huan Wei ◽  
Lina Yao ◽  
Lei Sun ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Rat Brain ◽  
Nanoporous Membrane ◽  
In Vivo Monitoring ◽  
Carbon Fiber Microelectrode

scholarly journals In Vivo Ambient Serotonin Measurements at Carbon-Fiber Microelectrodes

2017 ◽  
Vol 89 (18) ◽  
pp. 9703-9711 ◽  
Author(s):  
Aya Abdalla ◽  
Christopher W. Atcherley ◽  
Pavithra Pathirathna ◽  
Srimal Samaranayake ◽  
Beidi Qiang ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Carbon Fiber Microelectrodes

Ferricyanide-backfilled cylindrical carbon fiber microelectrodes for in vivo analysis with high stability and low polarized potential

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7154-7159 ◽  
Author(s):  
Peipei Zhong ◽  
Ping Yu ◽  
Kai Wang ◽  
Jie Hao ◽  
Junjie Fei ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
High Stability ◽  
Carbon Fiber Microelectrode ◽  
In Vivo Analysis ◽  
Carbon Fiber Microelectrodes

A ferricyanide-backfilled cylindrical carbon fiber microelectrode of high stability and low polarized potential was fabricated and used for in vivo analysis.

Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays

2018 ◽  
Vol 121 ◽  
pp. 142-150 ◽  
Author(s):  
Nuno R. Ferreira ◽  
Ana Ledo ◽  
João Laranjinha ◽  
Greg A. Gerhardt ◽  
Rui M. Barbosa
Keyword(s):  
Carbon Fiber ◽  
Rat Brain ◽  
Simultaneous Measurements ◽  
Nanocomposite Sensors

In Vivo Brain Glucose Measurements:  Differential Normal Pulse Voltammetry with Enzyme-Modified Carbon Fiber Microelectrodes

1996 ◽  
Vol 68 (24) ◽  
pp. 4358-4364 ◽  
Author(s):  
Larissa I. Netchiporouk ◽  
Nataliya F. Shram ◽  
Nicole Jaffrezic-Renault ◽  
Claude Martelet ◽  
Raymond Cespuglio
Keyword(s):  
Carbon Fiber ◽  
Brain Glucose ◽  
Pulse Voltammetry ◽  
Carbon Fiber Microelectrodes

In vivo measurement of tubular fluid ferrocyanide with carbon-fiber microelectrodes

1987 ◽  
Vol 252 (6) ◽  
pp. F1158-F1166
Author(s):  
L. C. Moore ◽  
C. Clausen ◽  
E. F. Bowden ◽  
A. Birzgalis
Keyword(s):  
Carbon Fiber ◽  
Proximal Tubule ◽  
Linear Response ◽  
Ion Concentration ◽  
Data Sampling ◽  
Sampling Device ◽  
Carbon Fiber Microelectrode ◽  
Carbon Fiber Microelectrodes

Techniques to construct carbon-fiber microelectrodes and to measure ferrocyanide ion concentration in single nephrons are described. The measurement involves polarizing an inert carbon-fiber microelectrode 500 mV positive with respect to a Ag-AgCl reference, while measuring the faradic current produced by the oxidation of ferrocyanide. A carbon fiber (5-7 micron diam) is heat sealed into a glass micropipette that is then sharpened, silanized, and electrochemically pretreated to minimize electrode degradation by protein. Circuit diagrams for an inexpensive voltage clamp-current monitor and a data sampling device are presented. The electrodes show a linear response to changes in ferrocyanide concentration in large and very small (20 nl) volumes in vitro. The electrodes were used in an electrochemical microassay to determine tubular fluid-to-plasma ferrocyanide concentration ratios and nephron filtration rates with proximal micropuncture samples. The results show excellent agreement with paired determinations using [3H]inulin. In vivo proximal tubule perfusion experiments show a rapid linear response to changes in tubular fluid ferrocyanide concentration. These electrodes permit rapid quantitative measurements of ferrocyanide concentration and water transport in the proximal tubule and may be useful in other biological systems.

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