Application of fast-scan cyclic voltammetry for the in vivo characterization of optically evoked dopamine in the olfactory tubercle of the rat brain

The Analyst ◽  
2016 ◽  
Vol 141 (12) ◽  
pp. 3746-3755 ◽  
Author(s):  
Ken T. Wakabayashi ◽  
Michael J. Bruno ◽  
Caroline E. Bass ◽  
Jinwoo Park
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Rat Brain ◽  
Olfactory Tubercle ◽  
Fast Scan Cyclic Voltammetry ◽  
Carbon Fiber Microelectrodes ◽  
In Vivo Characterization

Dopamine regulation in the rat brain olfactory tubercle was characterized by fast-scan cyclic voltammetry coupled with carbon–fiber microelectrodes and optogenetics.

Subsecond detection of guanosine using fast-scan cyclic voltammetry

The Analyst ◽  
2019 ◽  
Vol 144 (1) ◽  
pp. 249-257 ◽  
Author(s):  
Michael T. Cryan ◽  
Ashley E. Ross
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Electrochemical Characterization ◽  
Fast Scan Cyclic Voltammetry ◽  
Carbon Fiber Microelectrodes

We present the first electrochemical characterization of guanosine, a purinergic neuromodulator and neuroprotector, using fast-scan cyclic voltammetry at carbon-fiber microelectrodes.

Amine-Functionalized Carbon-Fiber Microelectrodes for Enhanced ATP Detection with Fast-Scan Cyclic Voltammetry

2021 ◽  
Author(s):  
Yuxin Li ◽  
Moriah E. Weese ◽  
Michael T. Cryan ◽  
Ashley E. Ross
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Adenosine Triphosphate ◽  
Fiber Surface ◽  
Fast Scan Cyclic Voltammetry ◽  
Extracellular Signaling ◽  
Amine Functionalized ◽  
Carbon Fiber Surface ◽  
Carbon Fiber Microelectrodes ◽  
Atp Detection

Here, we provide evidence that functionalizing the carbon-fiber surface with amines significantly improves direct electrochemical adenosine triphosphate (ATP) detection with fast-scan cyclic voltammetry (FSCV). ATP is an important extracellular signaling...

In vitro and in vivo characterization of [3H]CNS-5161—A use-dependent ligand for theN-methyl-d-aspartate receptor in rat brain

Synapse ◽  
2007 ◽  
Vol 61 (8) ◽  
pp. 577-586 ◽  
Author(s):  
Anat Biegon ◽  
Andrew Gibbs ◽  
Maritza Alvarado ◽  
Michele Ono ◽  
Scott Taylor
Keyword(s):  
Rat Brain ◽  
Aspartate Receptor ◽  
In Vivo Characterization

Fast Scan Cyclic Voltammetry As a DNA Sensor Using Carbon Fiber Microelectrodes

2020 ◽  
Vol MA2020-01 (52) ◽  
pp. 2837-2837
Author(s):  
Favian Alberto Liu ◽  
Thomas Michael Asrat ◽  
Alexander George Zestos
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Dna Sensor ◽  
Fast Scan Cyclic Voltammetry ◽  
Carbon Fiber Microelectrodes

Carbon Nanotube-Modified Carbon Fiber Microelectrodes for In Vivo Voltammetric Measurement of Ascorbic Acid in Rat Brain

2007 ◽  
Vol 79 (17) ◽  
pp. 6559-6565 ◽  
Author(s):  
Meining Zhang ◽  
Kun Liu ◽  
Ling Xiang ◽  
Yuqing Lin ◽  
Lei Su ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Carbon Nanotube ◽  
Carbon Fiber ◽  
Rat Brain ◽  
Voltammetric Measurement ◽  
Carbon Fiber Microelectrodes

scholarly journals Development of the Wireless Instantaneous Neurotransmitter Concentration System for intraoperative neurochemical monitoring using fast-scan cyclic voltammetry

2009 ◽  
Vol 111 (4) ◽  
pp. 712-723 ◽  
Author(s):  
Jonathan M. Bledsoe ◽  
Christopher J. Kimble ◽  
Daniel P. Covey ◽  
Charles D. Blaha ◽  
Filippo Agnesi ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Flow Injection ◽  
High Frequency ◽  
Dopamine Release ◽  
Functional Neurosurgery ◽  
Fast Scan Cyclic Voltammetry ◽  
Stimulation Of

Object Emerging evidence supports the hypothesis that modulation of specific central neuronal systems contributes to the clinical efficacy of deep brain stimulation (DBS) and motor cortex stimulation (MCS). Real-time monitoring of the neurochemical output of targeted regions may therefore advance functional neurosurgery by, among other goals, providing a strategy for investigation of mechanisms, identification of new candidate neurotransmitters, and chemically guided placement of the stimulating electrode. The authors report the development of a device called the Wireless Instantaneous Neurotransmitter Concentration System (WINCS) for intraoperative neurochemical monitoring during functional neurosurgery. This device supports fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM) for real-time, spatially and chemically resolved neurotransmitter measurements in the brain. Methods The FSCV study consisted of a triangle wave scanned between −0.4 and 1 V at a rate of 300 V/second and applied at 10 Hz. All voltages were compared with an Ag/AgCl reference electrode. The CFM was constructed by aspirating a single carbon fiber (r = 2.5 μm) into a glass capillary and pulling the capillary to a microscopic tip by using a pipette puller. The exposed carbon fiber (that is, the sensing region) extended beyond the glass insulation by ~ 100 μm. The neurotransmitter dopamine was selected as the analyte for most trials. Proof-of-principle tests included in vitro flow injection and noise analysis, and in vivo measurements in urethane-anesthetized rats by monitoring dopamine release in the striatum following high-frequency electrical stimulation of the medial forebrain bundle. Direct comparisons were made to a conventional hardwired system. Results The WINCS, designed in compliance with FDA-recognized consensus standards for medical electrical device safety, consisted of 4 modules: 1) front-end analog circuit for FSCV (that is, current-to-voltage transducer); 2) Bluetooth transceiver; 3) microprocessor; and 4) direct-current battery. A Windows-XP laptop computer running custom software and equipped with a Universal Serial Bus–connected Bluetooth transceiver served as the base station. Computer software directed wireless data acquisition at 100 kilosamples/second and remote control of FSCV operation and adjustable waveform parameters. The WINCS provided reliable, high-fidelity measurements of dopamine and other neurochemicals such as serotonin, norepinephrine, and ascorbic acid by using FSCV at CFM and by flow injection analysis. In rats, the WINCS detected subsecond striatal dopamine release at the implanted sensor during high-frequency stimulation of ascending dopaminergic fibers. Overall, in vitro and in vivo testing demonstrated comparable signals to a conventional hardwired electrochemical system for FSCV. Importantly, the WINCS reduced susceptibility to electromagnetic noise typically found in an operating room setting. Conclusions Taken together, these results demonstrate that the WINCS is well suited for intraoperative neurochemical monitoring. It is anticipated that neurotransmitter measurements at an implanted chemical sensor will prove useful for advancing functional neurosurgery.

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