Novel Method to Detect DNA Methylation Using Gold Nanoparticles Coupled with Enzyme-Linkage Reactions

2010 ◽  
Vol 82 (1) ◽  
pp. 229-233 ◽  
Author(s):  
Tao Liu ◽  
Jing Zhao ◽  
Dongmei Zhang ◽  
Genxi Li
Keyword(s):  
Dna Methylation ◽  
Gold Nanoparticles ◽  
Novel Method

scholarly journals Sensitive detection of methylated DNA and methyltransferase activity based on the lighting up of FAM-labeled DNA quenched fluorescence by gold nanoparticles

RSC Advances ◽  
2019 ◽  
Vol 9 (21) ◽  
pp. 12063-12069 ◽  
Author(s):  
Mohammad Ali Karimi ◽  
Mehdi Dadmehr ◽  
Morteza Hosseini ◽  
Behnaz Korouzhdehi ◽  
Fatemeh Oroojalian
Keyword(s):  
Dna Methylation ◽  
Gold Nanoparticles ◽  
Sensitive Detection ◽  
Fluorescence Recovery ◽  
Methyltransferase Activity ◽  
Au Nps ◽  
Methylated Dna ◽  
Novel Method

A novel method for detection of DNA methylation based on fluorescence recovery of FAM labeled DNA/Au NPs was introduced.

scholarly journals SYNTHESIS AND STABILITY OF RESVERATROL-CONJUGATED GOLD NANOPARTICLES MODIFIED WITH POLYETHYLENE GLYCOL

2020 ◽  
pp. 230-236
Author(s):  
RADITYA ISWANDANA ◽  
RICHA NURSELVIANA ◽  
SUTRIYO SUTRIYO
Keyword(s):  
Particle Size ◽  
Gold Nanoparticles ◽  
Polyethylene Glycol ◽  
Zeta Potential ◽  
High Performance ◽  
Entrapment Efficiency ◽  
Test Results ◽  
Novel Method ◽  
The Stability ◽  
Phosphate Buffered Saline

Objective: Gold nanoparticles (AuNPs) are highly useful for drug delivery, but their application is limited by their stability as they readily aggregate.This issue can be prevented by adding a stabilizing agent such as resveratrol (RSV), which is a polyphenol derived from plants, that is used to preventcancer. Therefore, we propose a novel method to prepare stable RSV-conjugated nanoparticles modified with polyethylene glycol (RSV-AuNP-PEG).Methods: In the first step, the Turkevich method was used to synthesize the AuNPs. Then, PEG was added as stabilizer agent and conjugated with RSV.The synthesized conjugates were characterized using ultraviolet-visible spectrophotometry, Fourier transform infrared spectroscopy, particle sizeanalysis, and high-performance liquid chromatography.Results: The obtained RSV-AuNP-PEG had a particle size of 83.93 nm with a polydispersity index (PDI) of 0.562 and formed a translucent purple-redfluid in solution. The zeta potential was −22.9 mV, and the highest entrapment efficiency was 75.86±0.66%. For comparison, the RSV-AuNP solutionwas purple and turbid, the particle size was 51.97 nm with a PDI of 0.694, and the zeta potential was −24.6 mV. The stability test results showed thatthe storage stability of RSV-AuNP-PEG was better than that of AuNP-RSV. Further, the RSV-AuNP-PEG was shown to be most stable in 2% bovine serumalbumin (BSA) while the AuNP-RSV was most stable in 2% BSA in phosphate-buffered saline pH 7.4.Conclusion: These results show that modification of RSV-conjugated AuNPs with PEG effectively prevents their aggregation in storage, but only incertain mediums.

Layer-by-layer self-assembly of polydopamine/gold nanoparticle/thiol coating as the stationary phase for open tubular capillary electrochromatography

2015 ◽  
Vol 7 (19) ◽  
pp. 8227-8234 ◽  
Author(s):  
Xiu-Ju Li ◽  
Qi-Feng Fu ◽  
Qi-Hui Zhang ◽  
Xue-Mei Jiang ◽  
Feng-Qing Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Stationary Phase ◽  
Gold Nanoparticle ◽  
Self Assembly ◽  
Capillary Electrochromatography ◽  
Phase Ratio ◽  
Layer By Layer ◽  
Novel Method ◽  
First Time ◽  
High Phase

A novel method for the preparation of permanent coating columns with a high phase ratio based on the multiple properties of PDA and LBL self-assembly of polydopamine/gold nanoparticles/thiols has been developed for the first time.

scholarly journals TET-mediated epimutagenesis of the Arabidopsis thaliana methylome

2017 ◽  
Author(s):  
Lexiang Ji ◽  
William T. Jordan ◽  
Xiuling Shi ◽  
Lulu Hu ◽  
Chuan He ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Arabidopsis Thaliana ◽  
Transcriptional Repression ◽  
Dna Methyltransferase ◽  
Developmental Defects ◽  
Altered Expression ◽  
Differentially Methylated Genes ◽  
Plant Genes ◽  
Novel Method ◽  
Phenotypic Variations

DNA methylation in the promoters of plant genes sometimes leads to transcriptional repression, and the wholesale removal of DNA methylation as seen in methyltransferase mutants results in drastic changes in gene expression and severe developmental defects. However, many cases of naturally-occurring DNA methylation variations have been reported, whereby the altered expression of differentially methylated genes is responsible for agronomically important traits. The ability to manipulate plant methylomes to generate populations of epigenetically distinct individuals could provide invaluable resources for breeding and research purposes. Here we describe “epimutagenesis”, a novel method to rapidly generate variation of DNA methylation through random demethylation of the Arabidopsis thaliana genome. This method involves the expression of a human Ten-eleven translocation (TET) enzyme, and results in widespread hypomethylation that can be inherited to subsequent generations, mimicking mutants in the maintenance DNA methyltransferase met1. Application of TET-mediated epimutagenesis to agriculturally significant plants may result in differential expression of alleles normally silenced by DNA methylation, uncovering previously hidden phenotypic variations.

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