Simultaneous detection of platelet-specific antibodies based on a photonic crystal-encoded suspension array

2016 ◽  
Vol 458 ◽  
pp. 72-77 ◽  
Author(s):  
Xin Kong ◽  
Baofen Ye ◽  
Zixue Yang ◽  
Baoan Chen ◽  
Yun Ling
Keyword(s):  
Photonic Crystal ◽  
Simultaneous Detection ◽  
Specific Antibodies ◽  
Suspension Array

Simultaneous Detection of Ochratoxin A and Fumonisin B1 in Cereal Samples Using an Aptamer–Photonic Crystal Encoded Suspension Array

2014 ◽  
Vol 86 (23) ◽  
pp. 11797-11802 ◽  
Author(s):  
Sun Yue ◽  
Xu Jie ◽  
Li Wei ◽  
Cao Bin ◽  
Wang Dou Dou ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Ochratoxin A ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Suspension Array ◽  
Cereal Samples

scholarly journals Virus-Coated Layer-by-Layer Colloids as a Multiplex Suspension Array for the Detection and Quantification of Virus-Specific Antibodies

2006 ◽  
Vol 52 (8) ◽  
pp. 1575-1583 ◽  
Author(s):  
Lars Toellner ◽  
Martin Fischlechner ◽  
Boris Ferko ◽  
Reingard Maria Grabherr ◽  
Edwin Donath
Keyword(s):  
Lipid Bilayer ◽  
Influenza A Virus ◽  
Influenza A ◽  
Dynamic Range ◽  
Flow Cytometric Analysis ◽  
Simultaneous Detection ◽  
Layer By Layer ◽  
Specific Antibodies ◽  
Suspension Array ◽  
Detection And Quantification

Abstract Background: Suspension array technology has surpassed ELISA for automated, simultaneous detection and quantification of soluble biomarkers such as virus-specific antibodies. We describe assays in which antigens are attached to a lipid bilayer surrounding color-coded particles. Methods: We used layer-by-layer technology to establish a multiplex suspension array with distinguishable microbeads coated with authentic viral surfaces to catch and quantify virus-specific antibodies in a flow cytometric analysis. Antigenic surfaces were generated by chimeric and wild-type baculoviruses plus 2 different influenza A virus subtypes fused to a lipid bilayer surrounding distinctly colored particles. Specificity of binding of chosen antibodies and sera was detected by immunofluorescence. Results of multiplex analysis were compared with results of ELISA. Results: Titrations of virus-specific antibodies in the multiplex suspension array demonstrated specific binding to the viral surface proteins. The multiplex suspension array gave positive results for up to log 5–diluted primary antibodies with an ∼5- to 10-fold reduced dynamic range compared with the respective ELISA. Conclusions: The bead-based multiplex suspension array is customizable and easy to establish. By displaying native influenza A virus surfaces and recombinant HIV-1 epitopes, the new assay provides a tool for the detection of major viral infections in humans.

Simultaneous detection of five biothreat agents in powder samples by a multiplexed suspension array

2009 ◽  
Vol 31 (3) ◽  
pp. 417-427 ◽  
Author(s):  
Jing Wang ◽  
Yu Yang ◽  
Lei Zhou ◽  
Jinglin Wang ◽  
Yongqiang Jiang ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Suspension Array ◽  
Powder Samples

Comparative study of the highly sensitive plasmonic sensor based on a D-Shaped photonic crystal fiber with silver or gold layers

2021 ◽  
Author(s):  
Bahar Meshginqalam ◽  
Jamal Barvestani
Keyword(s):  
Photonic Crystal ◽  
Comparative Study ◽  
Photonic Crystal Fiber ◽  
Structural Parameters ◽  
Simultaneous Detection ◽  
Plasmonic Sensor ◽  
Plasmonic Material ◽  
Sensor Performance ◽  
Crystal Fiber ◽  
Highly Sensitive

Abstract A highly sensitive D-shaped photonic crystal fiber sensor with circular lattice is proposed for external plasmonic sensing. The proposed design of plasmonic material in a D-shaped form effectively facilitates the excitation of surface plasmons and enhances the sensor performance. As a comparative study, two different plasmonic materials, gold and silver, are applied D-shapely on the fiber and the proposed sensor performance is numerically investigated and evaluated. Moreover, the optimized structural parameters such as air-hole diameters and the thickness of silver and gold layers are selected via simulation results which cause the highest sensitivity of 40000nm/RIU for the gold coated fiber using the wavelength interrogation method. Furthermore, the maximum figure of merit can reach 621.50RIU-1. Analytes with the refractive indices ranging from 1.34 to 1.39 can be detected by double-loss peak that is a more reliable method of simultaneous detection and verification of sensing characteristics. Due to its promising results, the proposed sensor can be widely useful in the area of chemical and biological sensing.

scholarly journals Comparison of Two Suspension Arrays for Simultaneous Detection of Five Biothreat Bacterial in Powder Samples

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Yu Yang ◽  
Jing Wang ◽  
Haiyan Wen ◽  
Hengchuan Liu
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Simultaneous Detection ◽  
Universal Primers ◽  
Universal Primer ◽  
Suspension Array ◽  
Specific Pathogen ◽  
Powder Samples ◽  
High Conservation ◽  
Species Specific

We have developed novel Bio-Plex assays for simultaneous detection ofBacillus anthracis, Yersinia pestis, Brucella spp., Francisella tularensis,andBurkholderia pseudomallei. Universal primers were used to amplify highly conserved region located within the 16S rRNA amplicon, followed by hybridized to pathogen-specific probes for identification of these five organisms. The other assay is based on multiplex PCR to simultaneously amplify five species-specific pathogen identification-targeted regions unique to individual pathogen. Both of the two arrays are validated to be flexible and sensitive for simultaneous detection of bioterrorism bacteria. However, universal primer PCR-based array could not identifyBacillus anthracis, Yersinia pestis,andBrucella spp.at the species level because of the high conservation of 16S rDNA of the same genus. The two suspension arrays can be utilized to detectBacillus anthracissterne spore andYersinia pestisEV76 from mimic “write powder” samples, they also proved that the suspension array system will be valuable tools for diagnosis of bacterial biothreat agents in environmental samples.

Simultaneous detection of five antibiotics in milk by high-throughput suspension array technology

Talanta ◽  
2011 ◽  
Vol 85 (2) ◽  
pp. 1160-1165 ◽  
Author(s):  
Pu Su ◽  
Nan Liu ◽  
Maoxiang Zhu ◽  
Baoan Ning ◽  
Ming Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
Simultaneous Detection ◽  
Suspension Array ◽  
Array Technology

Multiplex chemiluminescent immunoassay for screening of mycotoxins using photonic crystal microsphere suspension array

The Analyst ◽  
2014 ◽  
Vol 139 (4) ◽  
pp. 771-777 ◽  
Author(s):  
Kun Xu ◽  
Yue Sun ◽  
Wei Li ◽  
Jie Xu ◽  
Bin Cao ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Suspension Array ◽  
Chemiluminescent Immunoassay

scholarly journals Immunochromatographic Test for Simultaneous Serodiagnosis of Babesia caballi and B. equi Infections in Horses

2006 ◽  
Vol 13 (5) ◽  
pp. 553-555 ◽  
Author(s):  
Xiaohong Huang ◽  
Xuenan Xuan ◽  
Rodolfo A. Verdida ◽  
Shoufa Zhang ◽  
Naoaki Yokoyama ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Immunochromatographic Test ◽  
Babesia Caballi ◽  
Specific Antibodies ◽  
Immunosorbent Assays ◽  
Rhoptry Protein

ABSTRACT An immunochromatographic test for the simultaneous detection of Babesia caballi- and B. equi-specific antibodies (BceICT) was developed using a recombinant B. caballi 48-kDa rhoptry protein (rBc48) and a recombinant truncated B. equi merozoite antigen 2 (rEMA-2t). An evaluation of the ability of the BceICT to detect antibodies in sera from uninfected horses and experimentally infected horses showed high sensitivities and specificities of 83.3% (10/12 sera) and 92.9% (52/56 sera), respectively, for the anti-B. caballi antibody and 94.1% (16/17 sera) and 88.2% (45/51 sera), respectively, for the anti-B. equi antibody. Results from the detection of antibodies in field-collected sera indicated that the BceICT results corresponded with those of enzyme-linked immunosorbent assays (ELISA), showing 91.8% correspondence (67/73 sera) for B. caballi and 95.9% correspondence (70/73 sera) for B. equi, and that the BceICT results also corresponded with the ICT for B. caballi and for B. equi, both of which were 98.2% (55/56 sera). The comparable results of the ICT and ELISA and the simplicity and rapidity of the performance of the ICT suggest that the BceICT would be a feasible test for the simultaneous serodiagnosis of both agents of equine babesiosis in the field.

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