Magnetic Bead-Based Enzyme-Chromogenic Substrate System for Ultrasensitive Colorimetric Immunoassay Accompanying Cascade Reaction for Enzymatic Formation of Squaric Acid-Iron(III) Chelate

2014 ◽  
Vol 86 (10) ◽  
pp. 5061-5068 ◽  
Author(s):  
Wenqiang Lai ◽  
Dianping Tang ◽  
Junyang Zhuang ◽  
Guonan Chen ◽  
Huanghao Yang
Keyword(s):  
Magnetic Bead ◽  
Cascade Reaction ◽  
Squaric Acid ◽  
Chromogenic Substrate ◽  
Enzymatic Formation ◽  
Colorimetric Immunoassay

scholarly journals A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish

2020 ◽  
Vol 140 ◽  
pp. 111315
Author(s):  
Mònica Campàs ◽  
Jaume Reverté ◽  
Maria Rambla-Alegre ◽  
Katrina Campbell ◽  
Arjen Gerssen ◽  
...  
Keyword(s):  
Magnetic Bead ◽  
Colorimetric Immunoassay

scholarly journals Determination of microcystin-LR in surface water by a magnetic bead-based colorimetric immunoassay using antibody-conjugated gold nanoparticles

2016 ◽  
Vol 8 (1) ◽  
pp. 57-63 ◽  
Author(s):  
A.-C. Neumann ◽  
X. Wang ◽  
R. Niessner ◽  
D. Knopp
Keyword(s):  
Gold Nanoparticles ◽  
Surface Water ◽  
Magnetic Beads ◽  
Staining Method ◽  
Magnetic Bead ◽  
Colorimetric Immunoassay

Herein we describe the development of a homogeneous competitive colorimetric immunoassay using antigen-functionalized magnetic beads (MBs) and antibody-immobilized gold nanoparticles (AuNPs) combined with the established gold staining method for the determination of microcystin-leucine-arginine (MC-LR) in surface water.

scholarly journals Magnetic Bead-Based Colorimetric Immunoassay for Aflatoxin B1 Using Gold Nanoparticles

Sensors ◽  
2014 ◽  
Vol 14 (11) ◽  
pp. 21535-21548 ◽  
Author(s):  
Xu Wang ◽  
Reinhard Niessner ◽  
Dietmar Knopp
Keyword(s):  
Gold Nanoparticles ◽  
Aflatoxin B1 ◽  
Magnetic Bead ◽  
Colorimetric Immunoassay

scholarly journals Magnetic bead-based mimic enzyme-chromogenic substrate and silica nanoparticles signal amplification system for avian influenza A (H7N9) optical immunoassay

RSC Advances ◽  
2017 ◽  
Vol 7 (67) ◽  
pp. 41989-41999
Author(s):  
Dan Su ◽  
Hanyun Li ◽  
Jinlin Li ◽  
Yali Liu ◽  
Mi Peng ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Silica Nanoparticles ◽  
Signal Amplification ◽  
Rapid Detection ◽  
Influenza A ◽  
Magnetic Bead ◽  
Chromogenic Substrate ◽  
Amplification System ◽  
Signal Amplification System

Schematic illustration of the principle of the (a) colorimetric MB–MEMSCI and (b) optical MB–MEMSCI for rapid detection of H7N9 AIV.

Magnetic Bead-Based Reverse Colorimetric Immunoassay Strategy for Sensing Biomolecules

2013 ◽  
Vol 85 (14) ◽  
pp. 6945-6952 ◽  
Author(s):  
Zhuangqiang Gao ◽  
Mingdi Xu ◽  
Li Hou ◽  
Guonan Chen ◽  
Dianping Tang
Keyword(s):  
Magnetic Bead ◽  
Colorimetric Immunoassay

Factor XII Determinations in the Presence and Absence of Phospholipid Antibodies

1998 ◽  
Vol 79 (01) ◽  
pp. 87-90 ◽  
Author(s):  
D. W. Jones ◽  
M. Winter ◽  
M. J. Gallimore
Keyword(s):  
Lower Limit ◽  
Lupus Anticoagulant ◽  
Factor Xii ◽  
Chromogenic Substrate ◽  
Plasma Samples ◽  
Presence And Absence ◽  
Lower Limit Of Normal

SummaryFactor XII (FXII) levels were determined in plasma samples from 29 normal donors, 10 patients with inherited FXII deficiency (all lupus anticoagulant [LA] negative) and 67 LA positive patients, using clotting (FXIIct), chromogenic substrate (FXIIcs) and immunochemical (FXIIag) assays. Excellent correlations were obtained in the three FXII assays with the LA negative samples and between the FXIIcs and FXIIag assays in the LA positive samples. Correlations between both the FXIIcs and FXIIag with FXIIct in the LA positive patients were poor. Of 67 LA positive samples studied, 25 (37.3%) showed lower values in the FXIIct assay; 13 (19.4%) of these patients were pseudo FXII deficient with values of FXII below the lower limit of normal.These results indicate that a diagnosis of FXII deficiency can be made inappropriately in the presence of phospholipid antibodies and that such a diagnosis should not be made by FXIIct assay alone.

Visualization of von Willebrand Factor Multimers by Enzyme-Conjugated Secondary Antibodies

1986 ◽  
Vol 55 (02) ◽  
pp. 276-278 ◽  
Author(s):  
F Brosstad ◽  
Inge Kjønniksen ◽  
B Rønning ◽  
H Stormorken
Keyword(s):  
Von Willebrand Factor ◽  
Chromogenic Substrate ◽  
Agarose Electrophoresis ◽  
Secondary Antibodies ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Beta Galactosidase

SummaryA method for visualization of the multimeric forms of von Willebrand Factor (vWF) in plasma and platelets is described. The method is based upon: 1) Separation of the vWF multimers by SDS-agarose electrophoresis, 2) Subsequent blotting of the vWF multimers onto nitrocellulose, 3) Immunolocalization and visualization of the vWF pattern by the sequential incubation of the blot with a) primary vWF antiserum, b) peroxidase- or beta-galactosidase-conjugated secondary antibodies and a relevant chromogenic substrate.

Plasma Thrombin Assay using a Chromogenic Substrate in Disseminated Intravascular Coagulation due to Snake Bite Envenomation

1979 ◽  
Vol 41 (03) ◽  
pp. 544-552 ◽  
Author(s):  
R P Herrmann ◽  
P E Bailey
Keyword(s):  
Disseminated Intravascular Coagulation ◽  
Degradation Products ◽  
Snake Bite ◽  
Chromogenic Substrate ◽  
Coagulation Parameters ◽  
Fibrinogen Degradation Products ◽  
Intravascular Coagulation

SummaryUsing the chromogenic substrate, Tos-Gly-Pro-Arg-pNA-HCL (Chromozym TH, Boehringer Mannheim) plasma thrombin was estimated in six cases of envenomation by Australian elapid snakes. All patients manifested findings chracteristic of defibrination due to envenomation by these snakes. Fibrin-fibrinogen degradation products were grossly elevated, as was plasma thrombin in all cases.Following treatment with antivenene, all abnormal coagulation parameters returned rapidly towards normal by 24 hours and plasma thrombin disappeared.

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