scholarly journals Magnetic bead-based mimic enzyme-chromogenic substrate and silica nanoparticles signal amplification system for avian influenza A (H7N9) optical immunoassay

RSC Advances ◽  
2017 ◽  
Vol 7 (67) ◽  
pp. 41989-41999
Author(s):  
Dan Su ◽  
Hanyun Li ◽  
Jinlin Li ◽  
Yali Liu ◽  
Mi Peng ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Silica Nanoparticles ◽  
Signal Amplification ◽  
Rapid Detection ◽  
Influenza A ◽  
Magnetic Bead ◽  
Chromogenic Substrate ◽  
Amplification System ◽  
Signal Amplification System

Schematic illustration of the principle of the (a) colorimetric MB–MEMSCI and (b) optical MB–MEMSCI for rapid detection of H7N9 AIV.

Development of an antigen-ELISA and a colloidal gold–based immunochromatographic strip based on monoclonal antibodies for detection of avian influenza A(H5) viruses

2021 ◽  
pp. 104063872110275
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Linfang Cheng ◽  
Hangping Yao ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Disease Virus ◽  
Colloidal Gold ◽  
Rapid Detection ◽  
Influenza A ◽  
Field Investigation ◽  
Cross Reactivity ◽  
Detection Methods ◽  
Immunochromatographic Strip ◽  
Antigen Elisa

Avian influenza A(H5) viruses (avian IAVs) pose a major threat to the economy and public health. We developed an antigen-ELISA (ag-ELISA) and a colloidal gold–based immunochromatographic strip for the rapid detection of avian A(H5) viruses. Both detection methods displayed no cross-reactivity with other viruses (e.g., other avian IAVs, infectious bursal disease virus, Newcastle disease virus, infectious bronchitis virus, avian paramyxovirus). The ag-ELISA was sensitive down to 0.5 hemagglutinin (HA) units/100 µL of avian A(H5) viruses and 7.5 ng/mL of purified H5 HA proteins. The immunochromatographic strip was sensitive down to 1 HA unit/100 µL of avian A(H5) viruses. Both detection methods exhibited good reproducibility with CVs < 10%. For 200 random poultry samples, the sensitivity and specificity of the ag-ELISA were 92.6% and 98.8%, respectively, and for test strips were 88.9% and 98.3%, respectively. Both detection methods displayed high specificity, sensitivity, and stability, making them suitable for rapid detection and field investigation of avian A(H5) viruses.

An enzymatic signal amplification system for calorimetric studies of cellobiohydrolases

2010 ◽  
Vol 404 (2) ◽  
pp. 140-148 ◽  
Author(s):  
Leigh Murphy ◽  
Martin J. Baumann ◽  
Kim Borch ◽  
Matt Sweeney ◽  
Peter Westh
Keyword(s):  
Signal Amplification ◽  
Amplification System ◽  
Calorimetric Studies ◽  
Enzymatic Signal Amplification ◽  
Signal Amplification System
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