scholarly journals Determination of microcystin-LR in surface water by a magnetic bead-based colorimetric immunoassay using antibody-conjugated gold nanoparticles

2016 ◽  
Vol 8 (1) ◽  
pp. 57-63 ◽  
Author(s):  
A.-C. Neumann ◽  
X. Wang ◽  
R. Niessner ◽  
D. Knopp
Keyword(s):  
Gold Nanoparticles ◽  
Surface Water ◽  
Magnetic Beads ◽  
Staining Method ◽  
Magnetic Bead ◽  
Colorimetric Immunoassay

Herein we describe the development of a homogeneous competitive colorimetric immunoassay using antigen-functionalized magnetic beads (MBs) and antibody-immobilized gold nanoparticles (AuNPs) combined with the established gold staining method for the determination of microcystin-leucine-arginine (MC-LR) in surface water.

scholarly journals Magnetic Bead-Based Colorimetric Immunoassay for Aflatoxin B1 Using Gold Nanoparticles

Sensors ◽  
2014 ◽  
Vol 14 (11) ◽  
pp. 21535-21548 ◽  
Author(s):  
Xu Wang ◽  
Reinhard Niessner ◽  
Dietmar Knopp
Keyword(s):  
Gold Nanoparticles ◽  
Aflatoxin B1 ◽  
Magnetic Bead ◽  
Colorimetric Immunoassay

scholarly journals A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac

2021 ◽  
Author(s):  
Alexander Ecke ◽  
Tanja Westphalen ◽  
Jane Hornung ◽  
Michael Voetz ◽  
Rudolf J. Schneider
Keyword(s):  
Water Samples ◽  
Magnetic Beads ◽  
Limit Of Detection ◽  
Cost Effective ◽  
Magnetic Bead ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reference Analysis ◽  
Immunochemical Method ◽  
Synthetic Strategy

Abstract Increasing contamination of environmental waters with pharmaceuticals represents an emerging threat for the drinking water quality and safety. In this regard, fast and reliable analytical methods are required to allow quick countermeasures in case of contamination. Here, we report the development of a magnetic bead-based immunoassay (MBBA) for the fast and cost-effective determination of the analgesic diclofenac (DCF) in water samples, based on diclofenac-coupled magnetic beads and a robust monoclonal anti-DCF antibody. A novel synthetic strategy for preparation of the beads resulted in an assay that enabled for the determination of diclofenac with a significantly lower limit of detection (400 ng/L) than the respective enzyme-linked immunosorbent assay (ELISA). With shorter incubation times and only one manual washing step required, the assay demands for remarkably shorter time to result (< 45 min) and less equipment than ELISA. Evaluation of assay precision and accuracy with a series of spiked water samples yielded results with low to moderate intra- and inter-assay variations and in good agreement with LC–MS/MS reference analysis. The assay principle can be transferred to other, e.g., microfluidic, formats, as well as applied to other analytes and may replace ELISA as the standard immunochemical method. Graphical abstract

Determination of Amoxicillin Based on the Plasmon Resonance of Gold Nanoparticles

2012 ◽  
Vol 29 (11) ◽  
pp. 1329
Author(s):  
Fei LENG ◽  
Ying LI ◽  
Shunjun ZHEN ◽  
Yuanfang LI
Keyword(s):  
Gold Nanoparticles ◽  
Plasmon Resonance

Indirect Determination of Amikacin by Gold Nanoparticles as Redox Probe

2020 ◽  
Vol 17 ◽  
Author(s):  
Mansureh Alizadeh ◽  
Mandana Amiri ◽  
Abolfazl Bezaatpour
Keyword(s):  
Gold Nanoparticles ◽  
Bacterial Infections ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Cathodic Peak ◽  
Easy Method ◽  
Peak Current ◽  
Indirect Determination ◽  
Tract Infections

: Amikacin is an aminoglycoside antibiotic used for many gram-negative bacterial infections like infections in the urinary tract, infections in brain, lungs and abdomen. Electrochemical determination of amikacin is a challenge in electroanalysis because it shows no voltammetric peak at the surface of bare electrodes. In this approach, a very simple and easy method for indirect voltammetric determination of amikacin presented in real samples. Gold nanoparticles were electrodeposited at the surface of glassy carbon electrode in constant potential. The effect of several parameters such as time and potential of deposition, pH and scan rates on signal were studied. The cathodic peak current of Au3+ decreased with increasing amikacin concentration. Quantitative analysis of amikacin was performed using differential pulse voltammetry by following cathodic peak current of gold ions. Two dynamic linear ranges of 1.0 × 10−8–1.0 × 10-7 M and 5.0 × 10−7–1.0 × 10-3 M were obtained and limit of detection was estimated 3.0× 10−9 M. The method was successfully determined amikacin in pharmaceutical preparation and human serum. The effect of several interference in determination of amikacin was also studied.

Electrochemical aptasensor based on Mo2C/Mo2N and gold nanoparticles for determination of chlorpyrifos

2021 ◽  
Vol 188 (5) ◽  
Author(s):  
Zhenfeng Lin ◽  
Xin Liu ◽  
Yangzi Li ◽  
Changxiang Li ◽  
Liu Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Aptasensor

Determination of antibiotic resistance genes in a WWTP-impacted river in surface water, sediment, and biofilm: Influence of seasonality and water quality

2021 ◽  
pp. 144526
Author(s):  
Gabriela Reichert ◽  
Stephan Hilgert ◽  
Johannes Alexander ◽  
Júlio César Rodrigues de Azevedo ◽  
Tobias Morck ◽  
...  
Keyword(s):  
Water Quality ◽  
Antibiotic Resistance ◽  
Surface Water ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes

scholarly journals A system for the determination of surface water pCO2 in a highly variable environment, exemplified in the southern Baltic Sea

Oceanologia ◽  
2021 ◽  
Vol 63 (2) ◽  
pp. 276-282
Author(s):  
Marcin Stokowski ◽  
Przemysław Makuch ◽  
Krzysztof Rutkowski ◽  
Marcin Wichorowski ◽  
Karol Kuliński
Keyword(s):  
Surface Water ◽  
Baltic Sea ◽  
Variable Environment ◽  
Southern Baltic Sea ◽  
Southern Baltic
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