Amplified Detection of T4 Polynucleotide Kinase Activity by the Coupled λ Exonuclease Cleavage Reaction and Catalytic Assembly of Bimolecular Beacons

Ting Hou; Xiuzhong Wang; Xiaojuan Liu; Tingting Lu; Shufeng Liu; Feng Li

doi:10.1021/ac403458b

Amplified Detection of T4 Polynucleotide Kinase Activity by the Coupled λ Exonuclease Cleavage Reaction and Catalytic Assembly of Bimolecular Beacons

Analytical Chemistry ◽

10.1021/ac403458b ◽

2013 ◽

Vol 86 (1) ◽

pp. 884-890 ◽

Cited By ~ 86

Author(s):

Ting Hou ◽

Xiuzhong Wang ◽

Xiaojuan Liu ◽

Tingting Lu ◽

Shufeng Liu ◽

...

Keyword(s):

Kinase Activity ◽

Cleavage Reaction ◽

Polynucleotide Kinase ◽

T4 Polynucleotide Kinase

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Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

Analytical Methods ◽

10.1039/c9ay02283j ◽

2020 ◽

Vol 12 (6) ◽

pp. 807-812

Author(s):

Xu Wu ◽

Shuyi He ◽

Julia Xiaojun Zhao

Keyword(s):

Kinase Activity ◽

Sybr Green ◽

Fluorescence Assay ◽

Sybr Green I ◽

Label Free ◽

Cleavage Reaction ◽

Polynucleotide Kinase ◽

T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

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Sensitive detection of T4 polynucleotide kinase activity based on multifunctional magnetic probes and polymerization nicking reactions mediated hyperbranched rolling circle amplification

Biosensors and Bioelectronics ◽

10.1016/j.bios.2017.01.022 ◽

2017 ◽

Vol 91 ◽

pp. 631-636 ◽

Cited By ~ 29

Author(s):

Xia Li ◽

Xiaowen Xu ◽

Juan Song ◽

Qingwang Xue ◽

Chenzhong Li ◽

...

Keyword(s):

Kinase Activity ◽

Rolling Circle Amplification ◽

Sensitive Detection ◽

Rolling Circle ◽

Polynucleotide Kinase ◽

Magnetic Probes ◽

T4 Polynucleotide Kinase

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Sensitive fluorescence sensing of T4 polynucleotide kinase activity and inhibition based on DNA/polydopamine nanospheres platform

Talanta ◽

10.1016/j.talanta.2017.12.038 ◽

2018 ◽

Vol 180 ◽

pp. 271-276 ◽

Cited By ~ 16

Author(s):

Yao Cen ◽

Wen-Jing Deng ◽

Ru-Qin Yu ◽

Xia Chu

Keyword(s):

Kinase Activity ◽

Fluorescence Sensing ◽

Polynucleotide Kinase ◽

T4 Polynucleotide Kinase

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A nanoplatform based on metal–organic frameworks and coupled exonuclease reaction for the fluorimetric determination of T4 polynucleotide kinase activity and inhibition

Microchimica Acta ◽

10.1007/s00604-020-4194-y ◽

2020 ◽

Vol 187 (4) ◽

Author(s):

Yuying Chai ◽

Xia Cheng ◽

Guanhong Xu ◽

Fangdi Wei ◽

Jian Bao ◽

...

Keyword(s):

Kinase Activity ◽

Metal Organic Frameworks ◽

Fluorimetric Determination ◽

Polynucleotide Kinase ◽

Metal Organic ◽

T4 Polynucleotide Kinase

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DNAzyme-driven DNA walker biosensor for amplified electrochemical detection of T4 polynucleotide kinase activity and inhibition

Journal of Electroanalytical Chemistry ◽

10.1016/j.jelechem.2020.114470 ◽

2020 ◽

Vol 874 ◽

pp. 114470

Author(s):

Jingxiu Mao ◽

Xin Chen ◽

Huahuang Xu ◽

Xueqin Xu

Keyword(s):

Electrochemical Detection ◽

Kinase Activity ◽

Dna Walker ◽

Polynucleotide Kinase ◽

T4 Polynucleotide Kinase

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Detection of T4 polynucleotide kinase activity based on cationic conjugated polymer-mediated fluorescence resonance energy transfer

Biosensors and Bioelectronics ◽

10.1016/j.bios.2014.11.045 ◽

2015 ◽

Vol 66 ◽

pp. 316-320 ◽

Cited By ~ 27

Author(s):

Sai Lian ◽

Chenghui Liu ◽

Xiaobo Zhang ◽

Honghong Wang ◽

Zhengping Li

Keyword(s):

Energy Transfer ◽

Fluorescence Resonance Energy Transfer ◽

Conjugated Polymer ◽

Kinase Activity ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

Polynucleotide Kinase ◽

Fluorescence Resonance ◽

T4 Polynucleotide Kinase

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Highly specific fluorescence detection of T4 polynucleotide kinase activity via photo-induced electron transfer

Analytical Biochemistry ◽

10.1016/j.ab.2015.05.024 ◽

2015 ◽

Vol 485 ◽

pp. 18-24 ◽

Cited By ~ 14

Author(s):

Mangjuan Tao ◽

Zhilu Shi ◽

Rui Cheng ◽

Jing Zhang ◽

Baoxin Li ◽

...

Keyword(s):

Electron Transfer ◽

Fluorescence Detection ◽

Kinase Activity ◽

Specific Fluorescence ◽

Polynucleotide Kinase ◽

Photo Induced Electron Transfer ◽

T4 Polynucleotide Kinase

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A novel microchip electrophoresis laser induced fluorescence detection method for the assay of T4 polynucleotide kinase activity and inhibitors

Talanta ◽

10.1016/j.talanta.2019.05.032 ◽

2019 ◽

Vol 202 ◽

pp. 317-322 ◽

Cited By ~ 4

Author(s):

Yan Zhang ◽

Jingjin Zhao ◽

Shenyu Chen ◽

Shuting Li ◽

Shulin Zhao

Keyword(s):

Fluorescence Detection ◽

Kinase Activity ◽

Detection Method ◽

Microchip Electrophoresis ◽

Laser Induced Fluorescence ◽

Laser Induced Fluorescence Detection ◽

Polynucleotide Kinase ◽

T4 Polynucleotide Kinase

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Highly sensitive fluorescence assay of T4 polynucleotide kinase activity and inhibition via enzyme-assisted signal amplification

Analytical Biochemistry ◽

10.1016/j.ab.2014.07.008 ◽

2014 ◽

Vol 464 ◽

pp. 63-69 ◽

Cited By ~ 11

Author(s):

Mangjuan Tao ◽

Jing Zhang ◽

Yan Jin ◽

Baoxin Li

Keyword(s):

Signal Amplification ◽

Kinase Activity ◽

Fluorescence Assay ◽

Highly Sensitive ◽

Polynucleotide Kinase ◽

T4 Polynucleotide Kinase

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Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

Chemical Communications ◽

10.1039/c3cc47444e ◽

2014 ◽

Vol 50 (40) ◽

pp. 5313-5315 ◽

Cited By ~ 108

Author(s):

Hong-Zhang He ◽

Ka-Ho Leung ◽

Wei Wang ◽

Daniel Shiu-Hin Chan ◽

Chung-Hang Leung ◽

...

Keyword(s):

Kinase Activity ◽

Label Free ◽

Polynucleotide Kinase ◽

G Quadruplex ◽

T4 Polynucleotide Kinase

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