Combining Chemical Labeling, Bottom-Up and Top-Down Ion-Mobility Mass Spectrometry To Identify Metal-Binding Sites of Partially Metalated Metallothionein

2013 ◽  
Vol 85 (6) ◽  
pp. 3229-3237 ◽  
Author(s):  
Shu-Hua Chen ◽  
William K. Russell ◽  
David H. Russell
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Metal Binding ◽  
Binding Sites ◽  
Ion Mobility Mass Spectrometry ◽  
Top Down ◽  
Chemical Labeling ◽  
Bottom Up ◽  
Metal Binding Sites

scholarly journals Electron capture dissociation and drift tube ion mobility-mass spectrometry coupled with site directed mutations provide insights into the conformational diversity of a metamorphic protein

2015 ◽  
Vol 17 (16) ◽  
pp. 10538-10550 ◽  
Author(s):  
Sophie R. Harvey ◽  
Massimiliano Porrini ◽  
Robert C. Tyler ◽  
Cait E. MacPhee ◽  
Brian F. Volkman ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Ion Mobility ◽  
Drift Tube ◽  
Ion Mobility Mass Spectrometry ◽  
Top Down ◽  
Conformational Diversity ◽  
Metamorphic Protein

Ion mobility mass spectrometry can be combined with data from top-down sequencing to discern adopted conformations of proteins in the absence of solvent.

scholarly journals Mass Spectrometry-Based Structural Analysis of Cysteine-Rich Metal-Binding Sites in Proteins with MetaOdysseus R Software

2020 ◽  
Vol 20 (1) ◽  
pp. 776-785 ◽  
Author(s):  
Manuel David Peris-Díaz ◽  
Roman Guran ◽  
Ondrej Zitka ◽  
Vojtech Adam ◽  
Artur Krężel
Keyword(s):  
Mass Spectrometry ◽  
Structural Analysis ◽  
Metal Binding ◽  
Binding Sites ◽  
R Software ◽  
Metal Binding Sites

scholarly journals IMTBX and Grppr: Software for Top-Down Proteomics Utilizing Ion Mobility-Mass Spectrometry

2018 ◽  
Vol 90 (3) ◽  
pp. 2369-2375 ◽  
Author(s):  
Dmitry M. Avtonomov ◽  
Daniel A. Polasky ◽  
Brandon T. Ruotolo ◽  
Alexey I. Nesvizhskii
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Top Down

Resolution of a paradox by native mass spectrometry: facile occupation of all four metal binding sites in the dimeric zinc sensor SmtB

2013 ◽  
Vol 49 (8) ◽  
pp. 813-815 ◽  
Author(s):  
Frances D. L. Kondrat ◽  
Gregory R. Kowald ◽  
Charlotte A. Scarff ◽  
James H. Scrivens ◽  
Claudia A. Blindauer
Keyword(s):  
Mass Spectrometry ◽  
Metal Binding ◽  
Binding Sites ◽  
Native Mass Spectrometry ◽  
Zinc Sensor ◽  
Metal Binding Sites

scholarly journals Native Top-Down Mass Spectrometry and Ion Mobility MS for Characterizing the Cobalt and Manganese Metal Binding of α-Synuclein Protein

2018 ◽  
Vol 29 (9) ◽  
pp. 1870-1880 ◽  
Author(s):  
Piriya Wongkongkathep ◽  
Jong Yoon Han ◽  
Tae Su Choi ◽  
Sheng Yin ◽  
Hugh I. Kim ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Metal Binding ◽  
Top Down ◽  
Top Down Mass Spectrometry
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