Evaluation of Triterpene Glycoside Estrogenic Activity Using LC/MS and Immunoaffinity Extraction

2001 ◽  
Vol 73 (19) ◽  
pp. 4704-4710 ◽  
Author(s):  
Joelle Onorato ◽  
Jack D. Henion
Keyword(s):  
Triterpene Glycoside ◽  
Estrogenic Activity

Some Variation in Estrogenic Activity in Fresh and Dried White Clover Clones and the Ladino Variety

1960 ◽  
Vol 19 (4) ◽  
pp. 1143-1149 ◽  
Author(s):  
E. M. Bickoff ◽  
A. L. Livingston ◽  
A. N. Booth ◽  
C. R. Thompson ◽  
E. A. Hollowell ◽  
...  
Keyword(s):  
White Clover ◽  
Estrogenic Activity

Estrogenic Activity in Dehydrated and Suncured Forages

1960 ◽  
Vol 19 (1) ◽  
pp. 189-197 ◽  
Author(s):  
E. M. Bickoff ◽  
A. L. Livingston ◽  
A. N. Booth ◽  
A. P. Hendrickson ◽  
G. O. Kohler
Keyword(s):  
Estrogenic Activity

Estrogenic Activity of Soybean Forage1

1958 ◽  
Vol 17 (3) ◽  
pp. 787-791 ◽  
Author(s):  
Aden C. Magee ◽  
Gennard Matrone
Keyword(s):  
Estrogenic Activity

Stand Persistence and Estrogenic Activity of Hardseeded Subterranean Clover Cultivars for Southern and Central California

jpa ◽  
1991 ◽  
Vol 4 (1) ◽  
pp. 111-114 ◽  
Author(s):  
W. L. Graves ◽  
W. H. Weitkamp ◽  
M. R. George ◽  
G. R. Smith ◽  
B. L. Kay ◽  
...  
Keyword(s):  
Estrogenic Activity ◽  
Subterranean Clover ◽  
Central California

A Review of Analytical Methods for the Determination of Tibolone: Pharmacokinetics and Pharmaceutical Formulations Analysis and Application in Doping Control

2020 ◽  
Vol 17 (1) ◽  
pp. 31-39
Author(s):  
Marilene Lopes Ângelo ◽  
Fernanda de Lima Moreira ◽  
Ana Laura Araújo Santos ◽  
Hérida Regina Nunes Salgado ◽  
Magali Benjamim de Araújo
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Analytical Methods ◽  
Estrogenic Activity ◽  
Biological Fluids ◽  
Pharmaceutical Formulations ◽  
Doping Control ◽  
Specific Effects ◽  
In Doping

Background:: Tibolone is a synthetic steroid commercialized by Organon under the brand name Livial (Org OD14), which is used in hormone therapy for menopause management and treatment of postmenopausal osteoporosis. Tibolone is defined as a selective tissue estrogenic activity regulator (STEAR) demonstrating tissue-specific effects on several organs such as brain, breast, urogenital tract, endometrium, bone and cardiovascular system. Aims:: This work aims to (1) present an overview of important published literature on existing methods for the analysis of tibolone and/or its metabolites in pharmaceutical formulations and biological fluids and (2) to conduct a critical comparison of the analytical methods used in doping control, pharmacokinetics and pharmaceutical formulations analysis of tibolone and its metabolites. Results and conclusions: : The major analytical method described for the analysis of tibolone in pharmaceutical formulations is High Pressure Liquid Chromatography (HPLC) coupled with ultraviolet (UV) detection, while Liquid Chromatography (LC) or Gas Chromatography (GC) used in combination with Mass Spectrometry (MS) or tandem mass spectrometry (MS/MS) is employed for the analysis of tibolone and/or its metabolites in biological fluids.

Determination of estrogenic activity in landfill leachate by simplified yeast two-hybrid assay

2002 ◽  
Vol 4 (6) ◽  
pp. 1040-1046 ◽  
Author(s):  
Yasunori Kawagoshi ◽  
Yukiko Tsukagoshi ◽  
Isao Fukunaga
Keyword(s):  
Landfill Leachate ◽  
Estrogenic Activity ◽  
Yeast Two Hybrid ◽  
Two Hybrid

scholarly journals A New Anti-Estrogen Discovery Platform Identifies FDA-Approved Imidazole Anti-Fungal Drugs as Bioactive Compounds against ERα Expressing Breast Cancer Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 2915
Author(s):  
Manuela Cipolletti ◽  
Stefania Bartoloni ◽  
Claudia Busonero ◽  
Martina Parente ◽  
Stefano Leone ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bioactive Compounds ◽  
Estrogenic Activity ◽  
Estrogen Receptor Α ◽  
Breast Cancers ◽  
Cellular Models ◽  
Cancer Models ◽  
17Β Estradiol ◽  
Approved Drugs ◽  
Anti Fungal

17β-estradiol (E2) exerts its physiological effects through the estrogen receptor α (i.e., ERα). The E2:ERα signaling allows the regulation of cell proliferation. Indeed, E2 sustains the progression of ERα positive (ERα+) breast cancers (BCs). The presence of ERα at the BC diagnosis drives their therapeutic treatment with the endocrine therapy (ET), which restrains BC progression. Nonetheless, many patients develop metastatic BCs (MBC) for which a treatment is not available. Consequently, the actual challenge is to complement the drugs available to fight ERα+ primary and MBC. Here we exploited a novel anti-estrogen discovery platform to identify new Food and Drug Administration (FDA)-approved drugs inhibiting E2:ERα signaling to cell proliferation in cellular models of primary and MBC cells. We report that the anti-fungal drugs clotrimazole (Clo) and fenticonazole (Fenti) induce ERα degradation and prevent ERα transcriptional signaling and proliferation in cells modeling primary and metastatic BC. The anti-proliferative effects of Clo and Fenti occur also in 3D cancer models (i.e., tumor spheroids) and in a synergic manner with the CDK4/CDK6 inhibitors palbociclib and abemaciclib. Therefore, Clo and Fenti behave as “anti-estrogens”-like drugs. Remarkably, the present “anti-estrogen” discovery platform represents a valuable method to rapidly identify bioactive compounds with anti-estrogenic activity.

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