Rapid Water Sample Screening for Estrogenic Activity Using Live Yeast Cells

2007 ◽  
Author(s):  
E. Wozei ◽  
S. E. Borglin ◽  
H.-Y. N. Holman
Keyword(s):  
Water Sample ◽  
Estrogenic Activity ◽  
Yeast Cells ◽  
Live Yeast

scholarly journals Transcription Factor Clustering in Live Yeast Cells

2016 ◽  
Vol 110 (3) ◽  
pp. 231a
Author(s):  
Adam Wollman ◽  
Sviatlana Shashkova ◽  
Erik Hedlund ◽  
Stefan Hohmann ◽  
Mark C. Leake
Keyword(s):  
Transcription Factor ◽  
Yeast Cells ◽  
Live Yeast

Detection and removal of estrogencity in membrane biological reactors

2006 ◽  
Vol 6 (6) ◽  
pp. 19-26 ◽  
Author(s):  
J.Y. Hu ◽  
X. Chen
Keyword(s):  
Estrogenic Activity ◽  
Solid Phase ◽  
Treatment Plant ◽  
Endocrine Disrupting Compounds ◽  
Pilot Scale ◽  
Membrane Bioreactors ◽  
Yeast Cells ◽  
Endocrine Disrupting ◽  
And Behavior ◽  
Fate And Behavior

Three pilot-scale submerged membrane bioreactors (MBRs) in a local wastewater treatment plant (K, M and Z) were studied with the objective to compare the performance of pre-denitrification MBR systems in eliminating the estrogenic activity of the effluent of primary clarifier. A total of 5 batches of samples, which included influent, effluent, supernatant and sludge from the respective aerobic and anoxic tanks were collected over the span. They were investigated by using the developed solid-phase extraction (SPE) protocol coupled with a modified yeast-based estrogen screen (YES) assay. From the results, it could be seen that M MBR demonstrated the best endocrine disrupting compounds (EDCs) removal efficiency. The fate and behavior of EDCs in MBR systems were fairly understood with estrogenic activity formation dominating in the anoxic tank and removal dominating in the aerobic tank. It is believed that the sorption of EDCs onto the sludge as well as biodegradation of EDCs might be the key mechanisms for the EDCs removal. The low response of YES when dealing with influent samples was mainly due to the inhibition and antagonist effects induced by the influent samples on yeast cells.

The effect of yeast culture on rumen fermentation: Growth of the yeast in the rumen and the requirement for viable yeast cells

1993 ◽  
Vol 1993 ◽  
pp. 175-175 ◽  
Author(s):  
S.M. El Hassan ◽  
C.J. Newbold ◽  
R.J. Wallace
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rumen Fermentation ◽  
Bacterial Activity ◽  
Yeast Cells ◽  
Yeast Culture ◽  
Current Experiment ◽  
Microbial Protein ◽  
Live Yeast

Yeast culture (YC) based on Saccharomyces cerevisiae has been reported to stimulate bacterial activity within the rumen, leading to increases in ruminal fibre digestion and microbial protein flow from the rumen (Wallace and Newbold, 1992). Dawson (1987) suggested that S. cerevisiae might grow in the rumen. Newbold et al (1990) found no evidence for the growth of S. cerevisiae in the rumen of sheep when the numbers of live yeast in the rumen were measured at various times after a diet contain YC had been consumed. The current experiment was designed to investigate further the possibility that S. cerevisiae grows in the rumen and to establish the importance of viable yeast cells in the action of YC in the rumen.

EstraMonitor – A monitor for amperometric detection of estrogenic activity with Arxula adeninivorans yeast cells as the biocomponent

2012 ◽  
Vol 161 (1) ◽  
pp. 137-145 ◽  
Author(s):  
Ha Thi Minh Pham ◽  
Martin Giersberg ◽  
Steffen Uhlig ◽  
Gerold Hanke ◽  
Kirsten Simon ◽  
...  
Keyword(s):  
Estrogenic Activity ◽  
Amperometric Detection ◽  
Yeast Cells ◽  
Arxula Adeninivorans

scholarly journals Dark-field microscope stool analysis - its role in diagnosis of yeast overgrowth in gut

2017 ◽  
pp. 279-298
Author(s):  
Mária Klein-László
Keyword(s):  
Risk Factors ◽  
Candida Albicans ◽  
Gastrointestinal Tract ◽  
Invasive Candidiasis ◽  
Dark Field ◽  
Stool Culture ◽  
Intestinal Wall ◽  
Yeast Cells ◽  
Stool Analysis ◽  
Live Yeast

Not long after birth, yeast, predominantly Candida albicans, colonizes the epithelium of oral cavity and the whole gastrointestinal tract. C. albicans lives in yeast, a non-harming form, as a commensal member of the microbial flora, but may turn into patho?gen infective form under certain conditions that encourage its overgrowth. In this phase, it may damage the intestinal wall and enter the bloodstream, causing invasive candidiasis with high mortality rate. It is essential to recognize candidaemia and start the lifesaving therapy on time. Recognizing the risk factors which allow candida to overgrow is the most important step in preventing candida?s overgrowth and chronic candidiasis, the previous status of invasive candidiasis. If this recognition is missed, and the overgrowth advances, a question remains how to discover and treat it and in which phase it should be done. A stool culture requires time and proves the presence of live yeast cells only. If the live yeast cells are not present in the stool, the result of the culture will be negative. In this paper, the author presents her experience of stool analysis under dark-field microscope, as a rapid, easy to carry out method for detecting the presence of live or dead yeast cells and yeast overgrowth.

scholarly journals SIMILARITY OF THE KINETICS OF INVERTASE ACTION IN VIVO AND IN VITRO

1932 ◽  
Vol 15 (5) ◽  
pp. 491-495 ◽  
Author(s):  
J. M. Nelson ◽  
Elizabeth T. Palmer ◽  
B. G. Wilkes
Keyword(s):  
Yeast Cells ◽  
Enzymic Hydrolysis ◽  
Kinetics Of ◽  
Hydrolysis Of ◽  
Live Yeast

1. A method is given whereby the course of hydrolysis of sucrose by live yeast cells may be followed with precision equal to that found when invertase solutions prepared from autolyzed yeast are used to cause inversion. 2. The practical value of the equation of Nelson and Hitchcock as a means of following the course of enzymic hydrolysis of sucrose is hereby extended. 3. The inversion of sucrose by live yeast cells and by extracted invertase has been quantitatively compared. 4. The course of hydrolysis of sucrose by the invertase of Fleischmann's yeast has been found to be identical in vivo and in vitro.

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