Determination of estrogenic activity in landfill leachate by simplified yeast two-hybrid assay

2002 ◽  
Vol 4 (6) ◽  
pp. 1040-1046 ◽  
Author(s):  
Yasunori Kawagoshi ◽  
Yukiko Tsukagoshi ◽  
Isao Fukunaga
Keyword(s):  
Landfill Leachate ◽  
Estrogenic Activity ◽  
Yeast Two Hybrid ◽  
Two Hybrid

scholarly journals Concentration and Inhibitor Removal Methods for Determination of Estrogenic Activity in River Water using Yeast Two-Hybrid Test

2004 ◽  
Vol 27 (11) ◽  
pp. 679-684 ◽  
Author(s):  
Masahiro HOSOKAWA ◽  
Rui LIU ◽  
Takashi KAMEYA ◽  
Takashi KUBO ◽  
Kohei URANO
Keyword(s):  
River Water ◽  
Estrogenic Activity ◽  
Yeast Two Hybrid ◽  
Hybrid Test ◽  
Two Hybrid

Microbial degradation of estrogens using activated sludge and night soil-composting microorganisms

2004 ◽  
Vol 50 (8) ◽  
pp. 153-159 ◽  
Author(s):  
J.H. Shi ◽  
Y. Suzuki ◽  
S. Nakai ◽  
M. Hosomi
Keyword(s):  
Activated Sludge ◽  
Microbial Degradation ◽  
Estrogenic Activity ◽  
Degradation Products ◽  
Order Reaction ◽  
Yeast Two Hybrid ◽  
First Order ◽  
Natural Estrogens ◽  
17Β Estradiol ◽  
Two Hybrid

In order to investigate the potential for microbial degradation of estrogens, and the products formed, activated sludge collected from Korea (ASK) and night soil-composting microorganisms (NSCM) were used to degrade estrogens. Results showed that both ASK and NSCM degraded almost 100% of the natural estrogens estrone (E1), 17β-estradiol (E2), and estriol (E3) from initial concentrations of 20-25 mg/L, while synthetic estrogen, ethynylestradiol (EE2), was not degraded. Analysis of degradation products of E2 by using HPLC-ECD and a consecutive first-order reaction calculation confirmed that E2 was sequentially degraded to E1, which was further degraded to other unknown compounds by ASK and NSCM. We then used the yeast two-hybrid assay to show that the unknown degradation products did not appear to possess estrogenic activity when E1, E2 or E3 were degraded to below the detection limit after 14 days of incubation, indicating that ASK and NSCM not only degrade natural estrogens, but also remove their estrogenic activities.

Determination of Protein-Protein Interactions of ICln by the Yeast Two-Hybrid System

2001 ◽  
Vol 11 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Andreas Schmarda ◽  
Friedrich Fresser ◽  
Martin Gschwentner ◽  
Johannes Fürst ◽  
Markus Ritter ◽  
...  
Keyword(s):  
Hybrid System ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Two Hybrid

scholarly journals Determination of the Motif Responsible for Interaction between the Rice APETALA1/AGAMOUS-LIKE9 Family Proteins Using a Yeast Two-Hybrid System

1999 ◽  
Vol 120 (4) ◽  
pp. 1193-1204 ◽  
Author(s):  
Yong-Hwan Moon ◽  
Hong-Gyu Kang ◽  
Ji-Young Jung ◽  
Jong-Seong Jeon ◽  
Soon-Kee Sung ◽  
...  
Keyword(s):  
Hybrid System ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Two Hybrid

scholarly journals Estrogenic Activity of Branched 4-Nonylphenol Isomers Examined by Yeast Two-Hybrid Assay

2006 ◽  
Vol 52 (2) ◽  
pp. 132-141 ◽  
Author(s):  
Hirotaka Shioji ◽  
Shinji Tsunoi ◽  
Yosuke Kobayashi ◽  
Tatsushi Shigemori ◽  
Michihiko Ike ◽  
...  
Keyword(s):  
Estrogenic Activity ◽  
Yeast Two Hybrid ◽  
Two Hybrid

Determination of interactions between human thrombopoietin and its receptor MPL by yeast two-hybrid system and affinity biosensor

2000 ◽  
Vol 32 (5) ◽  
pp. 481-488 ◽  
Author(s):  
Dennis P.H. Hsieh ◽  
Susan Huxtable ◽  
Kit Fong Ng ◽  
Huih Min Chen ◽  
Paul W.K. Tsang ◽  
...  
Keyword(s):  
Hybrid System ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Two Hybrid

scholarly journals Estrogenic Activity of Coffee Constituents

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1401 ◽  
Author(s):  
Ryoiti Kiyama
Keyword(s):  
Cell Signaling ◽  
Binding Assay ◽  
Estrogenic Activity ◽  
Yeast Two Hybrid ◽  
Nitrogenous Compounds ◽  
Protein Assay ◽  
Gene Assay ◽  
Bone Protection ◽  
Signal Crosstalk ◽  
Two Hybrid

Here, the constituents of coffee with estrogenic activity are summarized by a comprehensive literature search, and their mechanisms of action for their physiological effects are discussed at the molecular and cellular levels. The estrogenic activity of coffee constituents, such as acids, caramelized products, carbohydrates, lignin, minerals, nitrogenous compounds, oil (lipids), and others, such as volatile compounds, was first evaluated by activity assays, such as animal tests, cell assay, ligand-binding assay, protein assay, reporter-gene assay, transcription assay, and yeast two-hybrid assay. Second, the health benefits associated with the estrogenic coffee constituents, such as bone protection, cancer treatment/prevention, cardioprotection, neuroprotection, and the improvement of menopausal syndromes, were summarized, including their potential therapeutic/clinical applications. Inconsistent results regarding mixed estrogenic/anti-estrogenic/non-estrogenic or biphasic activity, and unbeneficial effects associated with the constituents, such as endocrine disruption, increase the complexity of the effects of estrogenic coffee constituents. However, as the increase of the knowledge about estrogenic cell signaling, such as the types of specific signaling pathways, selective modulations of cell signaling, signal crosstalk, and intercellular/intracellular networks, pathway-based assessment will become a more realistic means in the future to more reliably evaluate the beneficial applications of estrogenic coffee constituents.

scholarly journals An Evaluation of Estrogenic Activity for 4-(methylthio)phenols on the Basis of Yeast Two Hybrid Assay

2005 ◽  
Vol 15 (4) ◽  
pp. 855-861
Author(s):  
Masanori TERASAKI ◽  
Takeshi KAZAMA ◽  
Shinya HASHIMOTO ◽  
Masakazu MAKINO
Keyword(s):  
Estrogenic Activity ◽  
Yeast Two Hybrid ◽  
Two Hybrid
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