Detection ofCryptosporidiumparvumUsing Oligonucleotide-Tagged Liposomes in a Competitive Assay Format

2001 ◽  
Vol 73 (13) ◽  
pp. 3162-3167 ◽  
Author(s):  
Mandy B. Esch ◽  
Antje J. Baeumner ◽  
Richard A. Durst
Keyword(s):  
Competitive Assay ◽  
Assay Format

scholarly journals SARS‐CoV ‐2 Antibody persistence in COVID ‐19 convalescent plasma donors: Dependency on assay format and applicability to serosurveillance

Transfusion ◽  
2021 ◽  
Author(s):  
Clara Di Germanio ◽  
Graham Simmons ◽  
Kathleen Kelly ◽  
Rachel Martinelli ◽  
Orsolya Darst ◽  
...  
Keyword(s):  
Antibody Persistence ◽  
Assay Format

Competitive Assay for Theophylline Based on an Abasic Site-Containing DNA Duplex Aptamer and a Fluorescent Ligand

2012 ◽  
Vol 18 (40) ◽  
pp. 12719-12724 ◽  
Author(s):  
Yusuke Sato ◽  
Yushuang Zhang ◽  
Seiichi Nishizawa ◽  
Takehiro Seino ◽  
Kodai Nakamura ◽  
...  
Keyword(s):  
Abasic Site ◽  
Dna Duplex ◽  
Competitive Assay ◽  
Fluorescent Ligand

Label-free and sensitive electrochemiluminescence aptasensor for the determination of 17β-estradiol based on a competitive assay with cDNA amplification

2014 ◽  
Vol 6 (17) ◽  
pp. 6796 ◽  
Author(s):  
Jing-Jing Zhang ◽  
Jun-Tao Cao ◽  
Gui-Fang Shi ◽  
Ke-Jing Huang ◽  
Yan-Ming Liu ◽  
...  
Keyword(s):  
Label Free ◽  
Competitive Assay ◽  
17Β Estradiol

scholarly journals Ultra-High Throughput Screen of Two-Million-Member Combinatorial Compound Collection in a Miniaturized, 1536-Well Assay Format

2000 ◽  
Vol 5 (3) ◽  
pp. 177-187 ◽  
Author(s):  
D. Dunn ◽  
M. Orlowski ◽  
P. McCoy ◽  
F. Gastgeb ◽  
K. Appell ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Successful Implementation ◽  
Screening Assay ◽  
Assay Format ◽  
Protease Enzyme ◽  
Compound Collection ◽  
Complete Survey ◽  
Well Assay ◽  
Active Class

Results of a complete survey of the more than 2-million-member Pharmacopeia compound collection in a 1536-well microvolume screening assay format are reported. A complete technology platform, enabling the performance of ultra-high throughput screening in a miniaturized 1536-well assay format, has been assembled and utilized. The platform consists of tools for performing microvolume assays, including assay plates, liquid handlers, optical imagers, and data management software. A fluorogenic screening assay for inhibition of a protease enzyme target was designed and developed using this platform. The assay was used to perform a survey screen of the Pharmacopeia compound collection for active inhibitors of the target enzyme. The results from the survey demonstrate the successful implementation of the ultra-high throughout platform for routine screening purposes. Performance of the assay in the miniaturized format is equivalent to that of a standard 96-well assay, showing the same dependence on kinetic parameters and ability to measure enzyme inhibition. The survey screen identified an active class of compounds within the Pharmacopeia compound collection. These results were confirmed using a standard 96-well assay.

scholarly journals Detection of Antibodies Directed to the N-Terminal Region of GAD Is Dependent on Assay Format and Contributes to Differences in the Specificity of GAD Autoantibody Assays for Type 1 Diabetes

Diabetes ◽  
2015 ◽  
Vol 64 (9) ◽  
pp. 3239-3246 ◽  
Author(s):  
Alistair J.K. Williams ◽  
Vito Lampasona ◽  
Michael Schlosser ◽  
Patricia W. Mueller ◽  
David L. Pittman ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Terminal Region ◽  
Assay Format
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