Label-free and sensitive electrochemiluminescence aptasensor for the determination of 17β-estradiol based on a competitive assay with cDNA amplification

2014 ◽  
Vol 6 (17) ◽  
pp. 6796 ◽  
Author(s):  
Jing-Jing Zhang ◽  
Jun-Tao Cao ◽  
Gui-Fang Shi ◽  
Ke-Jing Huang ◽  
Yan-Ming Liu ◽  
...  
Keyword(s):  
Label Free ◽  
Competitive Assay ◽  
17Β Estradiol

Label-Free Determination of the Cell Cycle Phase in Human Embryonic Stem Cells by Raman Microspectroscopy

2013 ◽  
Vol 85 (19) ◽  
pp. 8996-9002 ◽  
Author(s):  
Stanislav O. Konorov ◽  
H. Georg Schulze ◽  
James M. Piret ◽  
Michael W. Blades ◽  
Robin F. B. Turner
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Raman Microspectroscopy ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Label Free

Label-free RF biosensors for human cell dielectric spectroscopy

2009 ◽  
Vol 1 (6) ◽  
pp. 497-504 ◽  
Author(s):  
Claire Dalmay ◽  
Arnaud Pothier ◽  
Mathilde Cheray ◽  
Fabrice Lalloue ◽  
Marie-Odile Jauberteau ◽  
...  
Keyword(s):  
Human Cell ◽  
Cell Types ◽  
Label Free ◽  
Microwave Frequencies ◽  
Non Invasive ◽  
System Cell ◽  
Electromagnetic Characteristics ◽  
Biosensor Chip ◽  
Rf Signal

This paper presents an original biosensor chip allowing determination of intrinsic relative permittivity of biological cells at microwave frequencies. This sensor permits non-invasive cell identification and discrimination using an RF signal to probe intracellular medium of biological samples. Indeed, these sensors use an RF planar resonator that allows detection capabilities on less than 10 cells, thanks to the microscopic size of its sensitive area. Especially, measurements between 15 and 35 GHz show the ability label-free biosensors to differentiate two human cell types using their own electromagnetic characteristics. The real part of permittivity of cells changes from 20 to 48 for the nervous system cell types studied. The proposed biodetection method is detailed and we show how the accuracy and the repeatability of measurements have been improved to reach reproducible measurements.

Label Free Determination of Potassium Ions Using Crystal Violet and Thrombin-Binding Aptamer

2014 ◽  
Vol 47 (10) ◽  
pp. 1726-1736 ◽  
Author(s):  
Bin Zheng ◽  
Sheng Cheng ◽  
Huaze Dong ◽  
Haojun Liang ◽  
Jian Liu ◽  
...  
Keyword(s):  
Crystal Violet ◽  
Potassium Ions ◽  
Label Free

scholarly journals Biosensors for the Determination of SARS-CoV-2 Virus and Diagnosis of COVID-19 Infection

2022 ◽  
Vol 23 (2) ◽  
pp. 666
Author(s):  
Maryia Drobysh ◽  
Almira Ramanaviciene ◽  
Roman Viter ◽  
Chien-Fu Chen ◽  
Urte Samukaite-Bubniene ◽  
...  
Keyword(s):  
Current Trend ◽  
Analytical Signal ◽  
Diagnostic Methods ◽  
Detection Methods ◽  
Label Free ◽  
Free Mode ◽  
Rna Hybridisation ◽  
Antigen Antibody ◽  
Affinity Interaction

Monitoring and tracking infection is required in order to reduce the spread of the coronavirus disease 2019 (COVID-19), induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To achieve this goal, the development and deployment of quick, accurate, and sensitive diagnostic methods are necessary. The determination of the SARS-CoV-2 virus is performed by biosensing devices, which vary according to detection methods and the biomarkers which are inducing/providing an analytical signal. RNA hybridisation, antigen-antibody affinity interaction, and a variety of other biological reactions are commonly used to generate analytical signals that can be precisely detected using electrochemical, electrochemiluminescence, optical, and other methodologies and transducers. Electrochemical biosensors, in particular, correspond to the current trend of bioanalytical process acceleration and simplification. Immunosensors are based on the determination of antigen-antibody interaction, which on some occasions can be determined in a label-free mode with sufficient sensitivity.

scholarly journals Development of Label-free Immunosensor System toward Gender Determination of Fish

2021 ◽  
Vol 33 (9) ◽  
pp. 3233
Author(s):  
Haiyun Wu ◽  
Taro Sakurai ◽  
Yusuke Saito ◽  
Goro Yoshizaki ◽  
Yasutoshi Yoshiura ◽  
...  
Keyword(s):  
Label Free ◽  
Gender Determination

Encapsulation of hemin in Fe-based metal-organic frameworks and its application for the direct determination of aflatoxin M1

2021 ◽  
pp. 1-10
Author(s):  
F. Jahangiri-Dehaghani ◽  
H.R. Zare ◽  
Z. Shekari
Keyword(s):  
Electrochemical Impedance ◽  
Metal Organic Frameworks ◽  
Direct Determination ◽  
Differential Pulse ◽  
Aflatoxin M1 ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Selective Determination ◽  
Metal Organic

A label-free electrochemical aptasensor was constructed for the sensitive and selective determination of AFM1. For preparation of the aptasensor, the AFM1 aptamer was immobilised on the surface of a glassy carbon electrode modified with hemin encapsulated in Fe-based metal-organic frameworks (hemin@Fe-MIL-101). The morphology and the structure of Fe-MIL-101 and hemin@Fe-MIL-101 were evaluated by scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction and Brunauer-Emmett-Teller-N2 sorption methods. Electrochemical impedance spectroscopy and cyclic voltammetry were performed to monitor the fabrication process of the electrochemical aptasensor. The electrochemical reduction current of hemin encapsulated in Fe-MIL-101 serves as a signal for the quantitative determination of AFM1. Differential pulse voltammetry was done to determine the AFM1 concentration in the linear range of 1.0×10-1-100.0 ng/ml. The detection limit of AFM1 was estimated to be 4.6×10-2 ng/ml. Finally, the fabricated aptasensor was applied to determine AFM1 in raw and boiled milk samples.

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