Spacer length, label moiety interchange and probe pair orientation in a homogeneous solid-phase hybridization assay utilizing lanthanide chelate complementation

2014 ◽  
Vol 6 (14) ◽  
pp. 5360-5368 ◽  
Author(s):  
Susanne Lahdenperä ◽  
Julius Manninen ◽  
Laura Joki ◽  
Ulla Karhunen ◽  
Tero Soukka
Keyword(s):  
Dna Hybridization ◽  
Solid Phase ◽  
Spatial Multiplexing ◽  
Probe Pair ◽  
Hybridization Assay ◽  
Spacer Length ◽  
Lanthanide Chelate ◽  
Solid Phase Assay

We have studied parameters affecting DNA hybridization and lanthanide chelate complementation based signal formation in a separation-free solid-phase assay suitable for spatial multiplexing.

A rapid chemiluminescent DNA hybridization assay for the detection ofChlamydia trachomatis

1989 ◽  
Vol 4 (1) ◽  
pp. 357-366 ◽  
Author(s):  
J. M. Clyne ◽  
J. A. Running ◽  
M. Stempien ◽  
R. S. Stephens ◽  
H. Akhavan-Tafti ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Hybridization Assay ◽  
Ofchlamydia Trachomatis ◽  
Detection Ofchlamydia Trachomatis

114-P: Removing interference for solid phase assay: DTT treatment vs. heat inactivation

2009 ◽  
Vol 70 ◽  
pp. S69
Author(s):  
Patricia Willey ◽  
D. Phelan ◽  
G. Morris ◽  
T. Mohanakumar
Keyword(s):  
Solid Phase ◽  
Heat Inactivation ◽  
Solid Phase Assay

Detection of antinuclear antibodies by indirect immunofluorescence and by solid phase assay

2011 ◽  
Vol 10 (12) ◽  
pp. 801-808 ◽  
Author(s):  
Katrijn Op De Beeck ◽  
Pieter Vermeersch ◽  
Patrick Verschueren ◽  
René Westhovens ◽  
Godelieve Mariën ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Antinuclear Antibodies ◽  
Solid Phase ◽  
Solid Phase Assay

Rapid antiviral DNA-DNA hybridization assay for human cytomegalovirus

1990 ◽  
Vol 28 (3) ◽  
pp. 293-298 ◽  
Author(s):  
Wayne M. Dankner ◽  
David Scholl ◽  
Sylvia C. Stanat ◽  
Michael Martin ◽  
Robert L. Sonke ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Dna Hybridization ◽  
Hybridization Assay

scholarly journals Solid-Phase Assay for Luteinizing Hormone in Mouse Plasma

1976 ◽  
Vol 29 (2) ◽  
pp. 105 ◽  
Author(s):  
AA Gidley-Baird ◽  
BM Bindon
Keyword(s):  
Luteinizing Hormone ◽  
Sensitivity And Specificity ◽  
Solid Phase ◽  
Cross Reaction ◽  
Assay System ◽  
Mouse Plasma ◽  
Pregnant Mice ◽  
Solid Phase Assay ◽  
Made In ◽  
Pituitary Homogenate

A solid-phase tube assay for measuring LH levels in mouse plasma is described. The assay utilizes an antiserum to ovine LH and ovine LH standards and it measures LH levels in 20 III of plasma with a sensitivity of less than 0�6 ng/m!. Various parameters affecting the sensitivity and specificity of the assay were investigated. Serial dilutions of plasma from pregnant mice, a pituitary homogenate from mice and plasma from hypophysectomized mice, injected subcutaneously with ovine LB, ran parallel with ovine LH standards in plasma from hypophysectomized mice and plasma with low LH levels from intact mice. Ovine TSH showed about 12 % cross reaction in the assay system, whilst rat FSH and prolactin and also ovine FSH, prolactin and GH showed practically no cross reaction. Measurements of plasma LH levels have been made in hypophysectomized mice after injection with different vehicles containing 10 or 50llg LH or 50llg FSH per animal. Daily measurements of LH levels throughout pregnancy in the mouse show a rise in LH level prior to implantation and a further rise around mid-pregnancy which drops off sharply to levels which remain fairly constant until parturition when there is another rise.

Evaluation of a new solid phase assay "Capture-R" for detection of irregular red blood cell antibodies.

1990 ◽  
Vol 36 (6) ◽  
pp. 717-721
Author(s):  
Keisuke Nagamine ◽  
Hiroshi Aochi ◽  
Machiko Oshida ◽  
Satoru Hayashi ◽  
Yoshiyuki Kurata
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Solid Phase ◽  
Solid Phase Assay

scholarly journals Microscale ecotoxicity testing of Moselle river watershed (Lorraine Province, France) sediments

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Jean François Férard ◽  
Karen F. Burga Pérez ◽  
Christian Blaise ◽  
Alexandre Péry ◽  
Pakawadee Sutthivaiyakit ◽  
...  
Keyword(s):  
Correlation Analysis ◽  
Sublethal Effects ◽  
Solid Phase ◽  
Ecotoxicity Test ◽  
River Watershed ◽  
Ecotoxicity Testing ◽  
Sediment Particles ◽  
Secondary Consumers ◽  
Solid Phase Assay ◽  
Battery Of Bioassays

The ecotoxic potential of seven Moselle river watershed sediments was assessed with a battery of bioassays comprised of rapid phototrophic [LuminoTox solid phase (L-SPA) and elutriate (L-ELU) assays] and bacterial [Microtox solid phase assay (M-SPA)] exposure tests, as well as with two micro-invertebrate solid phase tests conducted with <em>Hydra</em> <em>attenuata</em> (lethal and sublethal effects solid phase assay, H<sub>L</sub>-SPA and H<sub>SL</sub>-SPA) and <em>Chironomus</em> <em>riparius</em>. Measured effects of sediments and their elutriates were varied and reflected responses that were ecotoxicity test-, endpoint- and site-dependent, suggesting some degree of risk toward benthic and water column organisms, respectively, at specific sites. Correlation analysis demonstrated that L-SPA and M-SPA ecotoxicity responses were significantly linked with the Hydra H<sub>SL</sub>-SPA assay, indicating their ability to predict ecotoxicity towards an invertebrate taxonomic group representing secondary consumers. While the L-SPA and M-SPA assays hold promise as rapid screens for sediment ecotoxicity, correlation analysis with grain size (L-SPA: r=-0.795, P=0.033; M-SPA: r=-0.73, P=0.07) points out that their responses can be influenced by the presence of fines (<em>i.e</em>., sediment particles ≤0.063 mm in size) and that this information is essential to properly interpret ecotoxicity data generated with these assays. Finally, notable differences observed in trophic level sensitivities once again recall the importance of employing a test battery to adequately appraise the ecotoxicity of sediments.

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