High-throughput extracellular pH monitoring and antibiotics screening by polymeric fluorescent sensor with LCST property

Methods ◽  
2019 ◽  
Vol 168 ◽  
pp. 51-61 ◽  
Author(s):  
Tingting Pan ◽  
Cheng Yang ◽  
Jiaze Li ◽  
Jiapei Jiang ◽  
Jiaxing Wen ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescent Sensor ◽  
Ph Monitoring ◽  
Extracellular Ph

High-Throughput and Real-Time Monitoring of Single-Cell Extracellular pH Based on Polyaniline Microarrays

2021 ◽  
Author(s):  
Lihui Yang ◽  
Xiaobo Liu ◽  
Bing Yin ◽  
Xunxun Deng ◽  
Xiaotong Lin ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
High Throughput ◽  
Extracellular Ph ◽  
Real Time Monitoring

A Redox-Activatable Fluorescent Sensor for the High-Throughput Quantification of Cytosolic Delivery of Macromolecules

2016 ◽  
Vol 129 (5) ◽  
pp. 1339-1343 ◽  
Author(s):  
Zhaohui Wang ◽  
Min Luo ◽  
Chengqiong Mao ◽  
Qi Wei ◽  
Tian Zhao ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescent Sensor ◽  
Cytosolic Delivery

Rational design of a turn-on fluorescent sensor for α-ketoglutaric acid in a microfluidic chip

2014 ◽  
Vol 5 (10) ◽  
pp. 4012-4016 ◽  
Author(s):  
Pengwei Jin ◽  
Changhong Jiao ◽  
Zhiqian Guo ◽  
Ye He ◽  
Shiqin Zhu ◽  
...  
Keyword(s):  
High Throughput ◽  
Microfluidic Chip ◽  
High Throughput Screening ◽  
Rational Design ◽  
Fluorescent Sensor ◽  
Fluorescent Chemosensors ◽  
Turn On

A rational design of turn-on fluorescent chemosensors for monitoring α-ketoglutaric acid has been developed with a microfluidic chip, indicative of a potential platform for high-throughput screening and monitoring of kinetics, especially in biological fields.

scholarly journals Ratiometric fluorescent pH-sensitive polymers for high-throughput monitoring of extracellular pH

RSC Advances ◽  
2016 ◽  
Vol 6 (52) ◽  
pp. 46134-46142 ◽  
Author(s):  
Liqiang Zhang ◽  
Fengyu Su ◽  
Xiangxing Kong ◽  
Fred Lee ◽  
Kevin Day ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Real Time ◽  
High Throughput ◽  
Ph Sensor ◽  
Ph Sensitive ◽  
Extracellular Ph ◽  
Water Soluble

A polymeric water-soluble extracellular pH sensor is developed with fluorescence ratiometric and cell membrane impermeable characters. The pH sensor enables us to exclusively detect the environmental pH of cells in real time.

scholarly journals Extracellular pH monitoring for use in closed-loop vagus nerve stimulation

2017 ◽  
Vol 15 (1) ◽  
pp. 016001 ◽  
Author(s):  
Simon C Cork ◽  
Amir Eftekhar ◽  
Khalid B Mirza ◽  
Claudio Zuliani ◽  
Konstantin Nikolic ◽  
...  
Keyword(s):  
Vagus Nerve ◽  
Nerve Stimulation ◽  
Vagus Nerve Stimulation ◽  
Closed Loop ◽  
Ph Monitoring ◽  
Extracellular Ph

A high‐throughput imaging platform to characterize extracellular pH in organotypic three‐dimensional in vitro models of liver cancer

2020 ◽  
Author(s):  
Lynn Jeanette Savic ◽  
Isabel Theresa Schobert ◽  
Charlie Alexander Hamm ◽  
Lucas Christoph Adam ◽  
Fahmeed Hyder ◽  
...  
Keyword(s):  
Liver Cancer ◽  
High Throughput ◽  
Three Dimensional ◽  
In Vitro Models ◽  
Extracellular Ph ◽  
High Throughput Imaging

scholarly journals In vivotumour extracellular pH monitoring using electron paramagnetic resonance: the effect of X-ray irradiation

2014 ◽  
Vol 27 (4) ◽  
pp. 453-458 ◽  
Author(s):  
Jonathan Goodwin ◽  
Katsuya Yachi ◽  
Masaki Nagane ◽  
Hironobu Yasui ◽  
Yusuke Miyake ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Ph Monitoring ◽  
Extracellular Ph ◽  
Paramagnetic Resonance ◽  
X Ray ◽  
Electron Paramagnetic

scholarly journals FLIM-Based Intracellular and Extracellular pH Measurements Using Genetically Encoded pH Sensor

Biosensors ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 340
Author(s):  
Alexander S. Goryashchenko ◽  
Alexey A. Pakhomov ◽  
Anastasia V. Ryabova ◽  
Igor D. Romanishkin ◽  
Eugene G. Maksimov ◽  
...  
Keyword(s):  
Cell Biology ◽  
Cytoplasmic Membrane ◽  
Transmembrane Domain ◽  
Fluorescent Sensor ◽  
Ph Sensor ◽  
Extracellular Ph ◽  
Live Cells ◽  
Ph Measurements ◽  
Quantitative Measurements

The determination of pH in live cells and tissues is of high importance in physiology and cell biology. In this report, we outline the process of the creation of SypHerExtra, a genetically encoded fluorescent sensor that is capable of measuring extracellular media pH in a mildly alkaline range. SypHerExtra is a protein created by fusing the previously described pH sensor SypHer3s with the neurexin transmembrane domain that targets its expression to the cytoplasmic membrane. We showed that with excitation at 445 nm, the fluorescence lifetime of both SypHer3s and SypHerExtra strongly depend on pH. Using FLIM microscopy in live eukaryotic cells, we demonstrated that SypHerExtra can be successfully used to determine extracellular pH, while SypHer3s can be applied to measure intracellular pH. Thus, these two sensors are suitable for quantitative measurements using the FLIM method, to determine intracellular and extracellular pH in a range from pH 7.5 to 9.5 in different biological systems.

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