scholarly journals Fluorescent Sensor for PH Monitoring Based on an i-Motif- – Switching Aptamer Containing a Tricyclic Cytosine Analogue (tC)

Molecules ◽  
2015 ◽  
Vol 20 (10) ◽  
pp. 18511-18525 ◽  
Author(s):  
Patrycja Bielecka ◽  
Bernard Juskowiak
Keyword(s):  
Fluorescent Sensor ◽  
Ph Monitoring

High-throughput extracellular pH monitoring and antibiotics screening by polymeric fluorescent sensor with LCST property

Methods ◽  
2019 ◽  
Vol 168 ◽  
pp. 51-61 ◽  
Author(s):  
Tingting Pan ◽  
Cheng Yang ◽  
Jiaze Li ◽  
Jiapei Jiang ◽  
Jiaxing Wen ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescent Sensor ◽  
Ph Monitoring ◽  
Extracellular Ph

Single-Molecule Imaging of Active Mitochondrial Nitroreductases using a Photo-Crosslinking Fluorescent Sensor

2019 ◽  
Author(s):  
Zacharias Thiel ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Subcellular Localization ◽  
Fluorescent Probe ◽  
Single Molecule ◽  
Mammalian Cells ◽  
Fluorescent Sensor ◽  
Biological Functions ◽  
Localization Microscopy ◽  
Drug Activation ◽  
Nitroreductase Activity ◽  
Single Molecule Localization Microscopy

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>

Single-Molecule Imaging of Active Mitochondrial Nitroreductases using a Photo-Crosslinking Fluorescent Sensor

2019 ◽  
Author(s):  
Zacharias Thiel ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Subcellular Localization ◽  
Fluorescent Probe ◽  
Single Molecule ◽  
Mammalian Cells ◽  
Fluorescent Sensor ◽  
Biological Functions ◽  
Localization Microscopy ◽  
Drug Activation ◽  
Nitroreductase Activity ◽  
Single Molecule Localization Microscopy

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>

Criteria of effective acid inhibition during antihelicobacter pharmacotherapy (according to the findings of express-gastro-pH-monitoring)

2019 ◽  
Vol 0 (3) ◽  
pp. 33-40
Author(s):  
I. G. Paliy ◽  
V. M. Сhernobrovyi ◽  
S. V. Zaika ◽  
I. V. Chernova ◽  
N. M. Kondratiuk
Keyword(s):  
Ph Monitoring ◽  
Acid Inhibition
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