Preparative scale cell-free expression systems: New tools for the large scale preparation of integral membrane proteins for functional and structural studies

Methods ◽  
2007 ◽  
Vol 41 (4) ◽  
pp. 355-369 ◽  
Author(s):  
Daniel Schwarz ◽  
Christian Klammt ◽  
Alexander Koglin ◽  
Frank Löhr ◽  
Birgit Schneider ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Large Scale ◽  
Integral Membrane Proteins ◽  
Free Expression ◽  
Structural Studies ◽  
Expression Systems ◽  
Preparative Scale ◽  
Large Scale Preparation ◽  
Scale Preparation

Cell-Free Expression of Integral Membrane Proteins for Structural Studies

2008 ◽  
pp. 141-164
Author(s):  
Christian Klammt ◽  
Daniel Schwarz ◽  
Ines Lehner ◽  
Solmaz Sobhanifar ◽  
Frank Lhr ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Free Expression ◽  
Structural Studies

Orthoamide und Iminiumsalze, LXXXIII [1]. Die Synthese von starken Formylierungsmitteln im präparativen Großmaßstab: Triformamid (Triformylamin) / Orthoamides and Iminium Salts LXXXIII [1]. The Synthesis of Strong Formylating Reagents on Large Preparative Scale: Triformamide (Triformylamine)

2014 ◽  
Vol 69 (3) ◽  
pp. 332-350
Author(s):  
Willi Kantlehner ◽  
Georg Ziegler ◽  
Oliver Scherr ◽  
Ralf Kreß ◽  
Sylvia Leonhardt
Keyword(s):  
Formic Acid ◽  
Low Temperatures ◽  
Large Scale ◽  
Acetyl Chloride ◽  
Inorganic Acid ◽  
Preparative Scale ◽  
Sodium Methanolate ◽  
Iminium Salts ◽  
Large Scale Preparation ◽  
Scale Preparation

The preparation of sodium diformamide (7) from formamide and sodium methanolate under azeotropic removal of methanol with cyclohexane, or from formamide and sodium ethanolate in ethanol, is described. Diformamide (8) can be prepared by treatment of sodium diformamide (7) with formic acid. Triformamide (triformylamine) (1) is formed in the reaction of sodium diformamide with inorganic acid halides such as SOCl2, SO2Cl2 or PCl3 in acetonitrile at low temperatures. Triformamide (1) can be prepared on a large scale by the action of methanesulfonyl chloride on finely powdered sodium diformamide (7) in acetonitrile. Tris(diformylamino)methane (4) can be formed as a side product. A procedure was developed for the large-scale preparation of N;N-diformylacetamide (6) from sodium diformamide and acetyl chloride. Triformamide (1) can be prepared from N;Ndiformylacetamide (6) and diformamide with good yields

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

3D Crystallization and Structural Studies of Integral Membrane Proteins in Lipidic Cubic phases

2000 ◽  
Vol 56 (s1) ◽  
pp. s83-s83
Author(s):  
P. Nollert ◽  
M. L. Chiu ◽  
M. C. Loewen ◽  
A. Royant ◽  
H. Behrhali ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Structural Studies ◽  
Cubic Phases

The occurrence of long chain polyprenols in leaves of plants of Rosaceae family and their isolation by time-extended liquid chromatography

1992 ◽  
Vol 70 (6) ◽  
pp. 448-454 ◽  
Author(s):  
Ewa Świeżewska ◽  
T. Chojnacki ◽  
W. J. Jankowski ◽  
K. Singh ◽  
J. Olsson
Keyword(s):  
Liquid Chromatography ◽  
Fatty Acid ◽  
Large Scale ◽  
Fatty Acid Esters ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Wet Weight ◽  
Rosaceae Family ◽  
Acid Esters ◽  
Long Chain

The long chain polyprenols composed of 30 and more isoprene units from leaves of plants belonging to the genera Potentilla and Rosa have been described. They occur in the form of fatty acid esters. The composition of polyprenol mixture was species dependent and its content reached ca. 0.5% wet weight. Large scale preparation of individual polyprenols from a natural polyprenol mixture was performed using time-extended liquid chromatography on the hydrophobic gel Lipidex-5000.Key words: long chain polyprenols, Rosaceae.

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