Cell-Free Expression of Integral Membrane Proteins for Structural Studies

2008 ◽  
pp. 141-164
Author(s):  
Christian Klammt ◽  
Daniel Schwarz ◽  
Ines Lehner ◽  
Solmaz Sobhanifar ◽  
Frank Lhr ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Free Expression ◽  
Structural Studies

3D Crystallization and Structural Studies of Integral Membrane Proteins in Lipidic Cubic phases

2000 ◽  
Vol 56 (s1) ◽  
pp. s83-s83
Author(s):  
P. Nollert ◽  
M. L. Chiu ◽  
M. C. Loewen ◽  
A. Royant ◽  
H. Behrhali ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Structural Studies ◽  
Cubic Phases

scholarly journals Lipid-like Peptides can Stabilize Integral Membrane Proteins for Biophysical and Structural Studies

ChemBioChem ◽  
2017 ◽  
Vol 18 (17) ◽  
pp. 1735-1742 ◽  
Author(s):  
Katharina Veith ◽  
Maria Martinez Molledo ◽  
Yasser Almeida Hernandez ◽  
Inokentijs Josts ◽  
Julius Nitsche ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Structural Studies

scholarly journals Application of Solution NMR to Structural Studies on α-Helical Integral Membrane Proteins

Molecules ◽  
2017 ◽  
Vol 22 (8) ◽  
pp. 1347 ◽  
Author(s):  
Dae-Won Sim ◽  
Zhenwei Lu ◽  
Hyung-Sik Won ◽  
Seu-Na Lee ◽  
Min-Duk Seo ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Solution Nmr ◽  
Structural Studies

scholarly journals High level cell-free expression and specific labeling of integral membrane proteins

2004 ◽  
Vol 271 (3) ◽  
pp. 568-580 ◽  
Author(s):  
Christian Klammt ◽  
Frank Lohr ◽  
Birgit Schafer ◽  
Winfried Haase ◽  
Volker Dotsch ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Free Expression ◽  
High Level

Comparison of lipidic carrier systems for integral membrane proteins – MsbA as case study

2019 ◽  
Vol 400 (11) ◽  
pp. 1509-1518 ◽  
Author(s):  
Dominique-Maurice Kehlenbeck ◽  
Inokentijs Josts ◽  
Julius Nitsche ◽  
Sebastian Busch ◽  
V. Trevor Forsyth ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Integral Membrane Proteins ◽  
Structural Studies ◽  
Long Term Stability ◽  
Activity Measurements ◽  
Protein Research ◽  
Protein Instability ◽  
Lipid Nanodiscs ◽  
Carrier Systems

Abstract Membrane protein research suffers from the drawback that detergents, which are commonly used to solubilize integral membrane proteins (IMPs), often lead to protein instability and reduced activity. Recently, lipid nanodiscs (NDs) and saposin-lipoprotein particles (Salipro) have emerged as alternative carrier systems that keep membrane proteins in a native-like lipidic solution environment and are suitable for biophysical and structural studies. Here, we systematically compare nanodiscs and Salipros with respect to long-term stability as well as activity and stability of the incorporated membrane protein using the ABC transporter MsbA as model system. Our results show that both systems are suitable for activity measurements as well as structural studies in solution. Based on our results we suggest screening of different lipids with respect to activity and stability of the incorporated IMP before performing structural studies.

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